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Comparison between in vitro gas production and in situ degradation for evaluating rumen dry matter degradation of maize grain and tropical grasses

  • R. M. Mauricio (a1), T.R. Tomich (a2), R. A. Filho (a3), R. B. Reis (a3), L.C. Gonçalves (a3), I. Borges (a3) and M. S. Dhanoa (a4)...

Summary

The in situ technique (NB) was compared to the in vitro gas production technique (Gas) in terms of ability to estimate the dry matter degradation (DMD) using high soluble substrates (maize grains) and low soluble substrates (four tropical forages). The experiments (in situ and in vitro) were carried out at the same time using the same cow for both techniques and DMD was estimated at 6, 12, 24, 48 & 96 h. The results showed that DMD from Gas were lower than NB DMD and the correlation for the maize group were lower than those of the grass group which indicated that Gas technique, closed system, has potential to analyse highly soluble substrates, probably, overcoming the particle losses effect demonstrated by the in situ technique.

Resumen

La técnica in situ (IS) fue comparada con la técnica in vitro de producción de gas (IV) en cuanto a su habilidad para estimar la degradación de la materia seca (DMS) empleando sustratos de alta solubilidad (grano de maíz) y sustratos de baja solubilidad (cuatro pastos tropicales). Los experimentos (in situ e in vitro) fueron conducidos al mismo tiempo empleando la misma vaca para ambas técnicas y la DMS estimada a las 6, 12, 24, 48 y 96 h. Los resultados mostraron que la DMS estimada a partir de IV fue menor que la DMS estimada por IS y la correlación para el grupo de grano de maíz fue menor que para el grupo de pastos, lo cual indica que la técnica IV, un sistema cerrado, tiene el potencial para estudiar sustratos altamente solubles, posiblemente evitando el problema de la pérdida de partículas, desventaja inherente a la técnica in situ.

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References

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Comparison between in vitro gas production and in situ degradation for evaluating rumen dry matter degradation of maize grain and tropical grasses

  • R. M. Mauricio (a1), T.R. Tomich (a2), R. A. Filho (a3), R. B. Reis (a3), L.C. Gonçalves (a3), I. Borges (a3) and M. S. Dhanoa (a4)...

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