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Vitamin A equivalency and apparent absorption of β-carotene in ileostomy subjects using a dual-isotope dilution technique

  • Carolien A. Van Loo-Bouwman (a1), Ton H. J. Naber (a1) (a2), Richard B. van Breemen (a3), Dongwei Zhu (a3), Heleen Dicke (a4), Els Siebelink (a5), Paul J. M. Hulshof (a5), Frans G. M. Russel (a6), Gertjan Schaafsma (a5) (a7) and Clive E. West (a1) (a5)...

Abstract

The objective was to quantify the vitamin A equivalency of β-carotene in two diets using a dual-isotope dilution technique and the apparent β-carotene absorption as measured by the oral–faecal balance technique. Seventeen healthy adults with an ileostomy completed the 4-week diet-controlled, cross-over intervention study. Each subject followed both diets for 2 weeks: a diet containing vegetables low in β-carotene content with supplemental β-carotene in salad dressing oil (‘oil diet’; mean β-carotene intake 3·1 mg/d) and a diet containing vegetables and fruits high in β-carotene content (‘mixed diet’; mean β-carotene intake 7·6 mg/d). Daily each subject consumed a mean of 190 μg [13C10]β-carotene and 195 μg [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of β-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum. Apparent absorption of β-carotene was determined with oral–faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]β-carotene in oil of 3·6:1 (95 % CI 2·8, 4·6) regardless of dietary matrices differences. The apparent absorption of (labelled and dietary) β-carotene from the ‘oil diet’ (30 %) was 1·9-fold higher than from the ‘mixed diet’ (16 %). This extrinsic labelling technique can measure precisely the vitamin A equivalency of β-carotene in oil capsules, but it does not represent the effect of different dietary matrices.

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Corresponding author

*Corresponding author: Dr Carolien A. Van Loo-Bouwman, fax +31 318413577, email Carolien.VanLooBouwman@hotmail.com

References

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Keywords

Vitamin A equivalency and apparent absorption of β-carotene in ileostomy subjects using a dual-isotope dilution technique

  • Carolien A. Van Loo-Bouwman (a1), Ton H. J. Naber (a1) (a2), Richard B. van Breemen (a3), Dongwei Zhu (a3), Heleen Dicke (a4), Els Siebelink (a5), Paul J. M. Hulshof (a5), Frans G. M. Russel (a6), Gertjan Schaafsma (a5) (a7) and Clive E. West (a1) (a5)...

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