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Various non-digestible saccharides increase intracellular calcium ion concentration in rat small-intestinal enterocytes

  • Takuya Suzuki (a1) and Hiroshi Hara (a1) (a2)

Abstract

We have previously shown that non-digestible saccharides (NDS) stimulate intestinal Ca absorption via tight junctions. However, the cellular mechanisms activated by the NDS are not yet known. We investigated the effects of four NDS, difructose anhydride (DFA) III, DFAIV, fructo-oligosaccharides, and maltitol, on intracellular Ca signalling in isolated rat small-intestinal enterocytes. The changes in intracellular Ca2+ concentration were measured before and after the addition of capric acid (7·5 or 15 mmol/l, a positive control), glycerol, or each NDS (1 or 10 mmol/l) to fura-2-loaded enterocytes. Treatment with capric acid or each NDS caused an immediate and dose-dependent rise in intracellular Ca2+ concentration. Mechanical and osmotic stimulation achieved by adding glycerol had no effect on intracellular Ca2+ concentration. The intracellular Ca2+ concentration in enterocytes treated with DFAIII and fructo-oligosaccharides reached a peak level at about 30 s after stimulation, but those treated with DFAIV and maltitol showed further increases after the initial rapid rise. The maximum change in intracellular Ca2+ concentration obtained by the application of maltitol was higher than that of DFAIII at 10 mmol/l. These findings suggest that each of the four NDS directly stimulates rat enterocytes, and increases intracellular Ca2+ concentration. Thus, molecular structure may be more important than the size of the NDS in the induction of Ca signalling in the cells.

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Copyright

Corresponding author

*Corresponding author: Dr Hiroshi Hara, fax +81 11 706 2504, email, hara@chem.agr.hokudai.ac.jp

References

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Berridge, MJ, Bootman, MD & Roderick, HL (2003) Calcium: calcium signalling: dynamics, homeostasis and remodelling. Nat Rev Mol Cell Biol 4, 517529
Bronner, F (1998) Calcium absorption – a paradigm for mineral absorption. J Nutr 128, 917920
Bronner, F & Pansu, D (1999) Nutritional aspects of calcium absorption. J Nutr 129, 912
Duncan, DB (1955) Multiple range and multiple F test. Biometrics 11, 142
Geoffrey, PW (2002) Carbohydrate. In Nutrition – a Health Promotion Approach, pp. 209229 [Aileen, P and James, R, editors]. New York: Oxford University Press Inc.
Grynkiewicz, G, Poenie, M & Tsien, RY (1985) A new generation of Ca 2+ indicators with greatly improved fluorescence properties. J Biol Chem 260, 34403450
Hara, H, Suzuki, T & Aoyama, Y (2000) Ingestion of the soluble dietary fibre, polydextrose, increases calcium absorption and bone mineralization in normal and total-gastrectomized rats. Br J Nutr 84, 655661
Karczewski, J & Groot, J (2000) Molecular physiology and pathophysiology of tight junctions III. Tight junction regulation by intracellular messengers: differences in response within and between epithelia. Am J Physiol 279, G660G665
Kishi, K, Goda, T & Takase, S (1996) Maltitol increases transepithelial diffusional transfer of Ca in rat ileum. Life Sci 59, 11331140
Lindmark, T, Kimura, Y & Artursson, P (1998) Absorption enhancement through intracellular regulation of tight junction permeability by medium chain fatty acids in Caco-2 cells. J Pharmacol Exp Ther 284, 362369
Ma, TY, Hoa, NT, Tran, DD, Bui, V, Pedram, A, Mills, S & Merryfield, M (2000) Cytochalasin B modulation of Caco-2 tight junction barrier: role of myosin light chain kinase. Am J Physiol 279, G875G885
Mineo, H, Hara, H, Kikuchi, H, Sakurai, H & Tomita, F (2001) Various indigestible saccharides enhance net calcium transport from the epithelium of the small and large intestine of rats in vitro. J Nutr 131, 32433246
Mineo, H, Hara, H, Shigematsu, N, Okuhara, Y & Tomita, F (2002) Melibiose, difructose anhydride III and difructose anhydride IV enhance net calcium absorption in rat small and large intestinal epithelium by increasing the passage of tight junctions in vitro. J Nutr 132, 33943399
Mineo, H, Hara, H & Tomita, F (2002) Sugar alcohols enhance calcium transport from rat small and large intestine epithelium in vitro. Dig Dis Sci 47, 13261333
Mitic, LL, Van Itallie, CM & Anderson, JM (2000) Molecular physiology and pathophysiology of tight junctions I. Tight junction structure and function: lessons from mutant animals and proteins. Am J Physiol 279, G250G254
Sawai, T, Lampman, R, Hua, Y, Segura, B, Drongowski, RA, Coran, AG & Harmon, CM (2002) Lysophosphatidylcholine alters enterocyte monolayer permeability via a protein kinase C/Ca 2+ mechanism. Pediatr Surg Int 18, 591594
Suzuki, T, Hara, H, Kasai, T & Tomita, F (1998) Effects of difructose anhydride III on calcium absorption in small and large intestines of rats. Biosci Biotechnol Biochem 62, 837841
Tomita, M, Hayashi, M & Awazu, S (1994) Comparison of absorption-enhancing effect between sodium caprate and disodium ethylenediaminetetraacetate in Caco-2 cells. Biol Pharm Bull 17, 753755
Tomita, M, Hayashi, M & Awazu, S (1996) Absorption-enhancing mechanism of EDTA, caprate, and decanoylcarnitine in Caco-2 cells. J Pharm Sci 85, 608611
van den Heuvel, EG, Muys, T, van Dokkum, W & Schaafsma, G (1999) Oligofructose stimulates calcium absorption in adolescents. Am J Clin Nutr 69, 544548
Vetter, SW & Leclerc, E (2003) Novel aspects of calmodulin target recognition and activation. Eur J Biochem 270, 404414
Younes, H, Demigne, C & Remesy, C (1996) Acidic fermentation in the caecum increases absorption of calcium and magnesium in the large intestine of the rat. Br J Nutr 75, 301314

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Various non-digestible saccharides increase intracellular calcium ion concentration in rat small-intestinal enterocytes

  • Takuya Suzuki (a1) and Hiroshi Hara (a1) (a2)

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