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Supplementation of branched-chain amino acids to a reduced-protein diet improves growth performance in piglets: involvement of increased feed intake and direct muscle growth-promoting effect

  • Liufeng Zheng (a1), Hongkui Wei (a1) (a2), Chuanshang Cheng (a1), Quanhang Xiang (a1), Jiaman Pang (a1) and Jian Peng (a1) (a2)...

Abstract

The aim of this study was to investigate whether supplementing branched-chain amino acids (AA) (BCAA) along with a reduced-protein diet increases piglet growth, and whether elevated feed intake and muscle growth-promoting effect contribute to this improvement. In Expt 1, twenty-eight weanling piglets were randomly fed one of the following four diets: a positive control (PC) diet, a reduced-protein negative control (NC) diet, an NC diet supplemented with BCAA to the same levels as in the PC diet (test 1 (T1)) and an NC diet supplemented with a 2-fold dose of BCAA in T1 diet (test 2 (T2)) for 28 d. In Expt 2, twenty-one weanling piglets were randomly assigned to NC, T1 and pair-fed T1 (P) groups. NC and T1 diets were the same as in Expt 1, whereas piglets in the P group were individually pair-fed with the NC group. In Expt 1, the NC group had reduced piglet growth and feed intake compared with the PC group, which were restored in T1 and T2 groups, but no differences were detected between T1 and T2 groups. In Expt 2, T1 and P groups showed increases in growth and mass of some muscles compared with the NC group. Increased feed intake after BCAA supplementation was associated with increased mRNA expressions of agouti-related peptide and co-express neuropeptide Y (NPY) and phosphorylation of mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase 1 (S6K1), as well as decreased mRNA expressions of melanocortin-4 receptor and cocaine- and amphetamine-regulated transcript and phosphorylation of eukaryotic initiation factor 2α in the hypothalamus. No differences were observed among PC, T1 and T2 groups except for higher NPY mRNA expression in the T2 group than in the PC group (Expt 1). Phosphorylation of mTOR and S6K1 in muscle was enhanced after BCAA supplementation, which was independent of change in feed intake (Expt 2). In conclusion, supplementing BCAA to reduced-protein diets increases feed intake and muscle mass, and contributes to better growth performance in piglets.

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Corresponding author

* Corresponding author: J. Peng, fax +86 278 7281 378, email pengjian@mail.hzau.edu.cn

References

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1. McCracken, BA, Gaskins, HR, Ruwe-Kaiser, PJ, et al. (1995) Diet-dependent and diet-independent metabolic responses underlie growth stasis of pigs at weaning. J Nutr 125, 28382845.
2. Brown, DC, Maxwell, CV, Erf, GF, et al. (2006) The influence of different management systems and age on intestinal morphology, immune cell numbers and mucin production from goblet cells in post-weaning pigs. Vet Immunol Immunopathol 111, 187198.
3. Rose, N, Larour, G, Le Diguerher, G, et al. (2003) Risk factors for porcine post-weaning multisystemic wasting syndrome (PMWS) in 149 French farrow-to-finish herds. Prev Vet Med 61, 209225.
4. Nyachoti, CM, Omogbenigun, FO, Rademacher, M, et al. (2006) Performance responses and indicators of gastrointestinal health in early-weaned pigs fed low-protein amino acid-supplemented diets. J Anim Sci 84, 125134.
5. Opapeju, FO, Krause, DO, Payne, RL, et al. (2009) Effect of dietary protein level on growth performance, indicators of enteric health, and gastrointestinal microbial ecology of weaned pigs induced with postweaning colibacillosis. J Anim Sci 87, 26352643.
6. Kong, X, Wu, G, Liao, Y, et al. (2007) Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance, serum metabolites and intestinal health in early-weaned piglets. Livest Sci 108, 272275.
7. Heo, JM, Opapeju, FO, Pluske, JR, et al. (2013) Gastrointestinal health and function in weaned pigs: a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds. J Anim Physiol Anim Nutr (Berl) 97, 207237.
8. Rist, V, Weiss, E, Eklund, M, et al. (2013) Impact of dietary protein on microbiota composition and activity in the gastrointestinal tract of piglets in relation to gut health: a review. Animal 7, 10671078.
9. National Research Council (1998) Nutrient Requirements of Swine, 10th rev. ed. Washington, DC: The National Academies Press.
10. Lordelo, M, Gaspar, A, Le Bellego, L, et al. (2008) Isoleucine and valine supplementation of a low-protein corn-wheat-soybean meal-based diet for piglets: growth performance and nitrogen balance. J Anim Sci 86, 29362941.
11. Zhang, S, Qiao, S, Ren, M, et al. (2013) Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs. Amino Acids 45, 11911205.
12. Le Bellego, L & Noblet, J (2002) Performance and utilization of dietary energy and amino acids in piglets fed low protein diets. Livest Prod Sci 76, 4558.
13. Nørgaard, JV & Fernández, JA (2009) Isoleucine and valine supplementation of crude protein-reduced diets for pigs aged 5-8 weeks. Anim Feed Sci Tech 154, 248253.
14. Gloaguen, M, Le Floc’h, N, Brossard, L, et al. (2011) Response of piglets to the valine content in diet in combination with the supply of other branched-chain amino acids. Animal 5, 17341742.
15. Gloaguen, M, Le Floc’h, N, Corrent, E, et al. (2012) Providing a diet deficient in valine but with excess leucine results in a rapid decrease in feed intake and modifies the postprandial plasma amino acid and alpha-keto acid concentrations in pigs. J Anim Sci 90, 31353142.
16. Gloaguen, M, Le Floc’h, N, Corrent, E, et al. (2013) Meal patterns in relation to the supply of branched-chain amino acids in pigs. J Anim Sci 91, 292297.
17. Kamata, S, Yamamoto, J, Kamijo, K, et al. (2014) Dietary deprivation of each essential amino acid induces differential systemic adaptive responses in mice. Mol Nutr Food Res 58, 13091321.
18. Wiltafsky, MK, Pfaffl, MW & Roth, FX (2010) The effects of branched-chain amino acid interactions on growth performance, blood metabolites, enzyme kinetics and transcriptomics in weaned pigs. Br J Nutr 103, 964976.
19. Soumeh, EA, van Milgen, J, Sloth, NM, et al. (2015) The optimum ratio of standardized ileal digestible leucine to lysine for 8 to 12 kg female pigs. J Anim Sci 93, 22182224.
20. Yin, Y, Yao, K, Liu, Z, et al. (2010) Supplementing L-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs. Amino Acids 39, 14771486.
21. Murgas Torrazza, R, Suryawan, A, Gazzaneo, MC, et al. (2010) Leucine supplementation of a low-protein meal increases skeletal muscle and visceral tissue protein synthesis in neonatal pigs by stimulating mTOR-dependent translation initiation. J Nutr 140, 21452152.
22. Suryawan, A, Torrazza, RM, Gazzaneo, MC, et al. (2012) Enteral leucine supplementation increases protein synthesis in skeletal and cardiac muscles and visceral tissues of neonatal pigs through mTORC1-dependent pathways. Pediatr Res 71, 324331.
23. Escobar, J, Frank, JW, Suryawan, A, et al. (2005) Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation. Am J Physiol Endocrinol Metab 288, E914E921.
24. Escobar, J, Frank, JW, Suryawan, A, et al. (2006) Regulation of cardiac and skeletal muscle protein synthesis by individual branched-chain amino acids in neonatal pigs. Am J Physiol Endocrinol Metab 290, E612E621.
25. Cota, D, Proulx, K, Smith, KAB, et al. (2006) Hypothalamic mTOR signaling regulates food intake. Science 312, 927930.
26. Gietzen, DW, Hao, S & Anthony, TG (2007) Mechanisms of food intake repression in indispensable amino acid deficiency. Annu Rev Nutr 27, 6378.
27. Maurin, A-C, Benani, A, Lorsignol, A, et al. (2014) Hypothalamic eIF2α signaling regulates food intake. Cell Rep 6, 438444.
28. Morton, G, Cummings, D, Baskin, D, et al. (2006) Central nervous system control of food intake and body weight. Nature 443, 289295.
29. National Research Council (2012) Nutrient Requirements of Swine, 11th rev. ed. Washington, DC: The National Academies Press.
30. Swamy, H, Smith, T & MacDonald, E (2004) Effects of feeding blends of grains naturally contaminated with mycotoxins on brain regional neurochemistry of starter pigs and broiler chickens. J Anim Sci 82, 21312139.
31. Association of Official Analytical Chemists (2003) Official Methods of Analysis, 17th rev. ed. Arlington, VA: AOAC.
32. Wang, X, Wei, H, Cao, J, et al. (2015) Metabolomics analysis of muscle from piglets fed low protein diets supplemented with branched chain amino acids using HPLC-high resolution MS. Electrophoresis 36, 22502258.
33. Livak, KJ & Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25, 402408.
34. Ettle, T & Roth, F (2004) Specific dietary selection for tryptophan by the piglet. J Anim Sci 82, 11151121.
35. Blouet, C, Jo, YH, Li, X, et al. (2009) Mediobasal hypothalamic leucine sensing regulates food intake through activation of a hypothalamus-brainstem circuit. J Neurosci 29, 83028311.
36. Maurin, A-C, Jousse, C, Averous, J, et al. (2005) The GCN2 kinase biases feeding behavior to maintain amino acid homeostasis in omnivores. Cell Metab 1, 273277.
37. Hao, S, Sharp, JW, Ross-Inta, CM, et al. (2005) Uncharged tRNA and sensing of amino acid deficiency in mammalian piriform cortex. Science 307, 17761778.
38. Martins, L, Fernández-Mallo, D, Novelle, MG, et al. (2012) Hypothalamic mTOR signaling mediates the orexigenic action of ghrelin. PLOS ONE 7, e46923.
39. Morrison, CD, Xi, X, White, CL, et al. (2007) Amino acids inhibit Agrp gene expression via an mTOR-dependent mechanism. Am J Physiol Endocrinol Metab 293, E165E171.
40. Meijer, AJ & Dubbelhuis, PF (2004) Amino acid signalling and the integration of metabolism. Biochem Biophys Res Commun 313, 397403.
41. Columbus, DA, Fiorotto, ML & Davis, TA (2015) Leucine is a major regulator of muscle protein synthesis in neonates. Amino Acids 47, 259270.
42. Atherton, PJ, Smith, K, Etheridge, T, et al. (2010) Distinct anabolic signalling responses to amino acids in C2C12 skeletal muscle cells. Amino Acids 38, 15331539.
43. Hara, K, Yonezawa, K, Weng, Q-P, et al. (1998) Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism. J Biol Chem 273, 1448414494.

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