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A single injection of vitamin D3 improves insulin sensitivity and β-cell function but not muscle damage or the inflammatory and cardiovascular responses to an acute bout of resistance exercise in vitamin D-deficient resistance-trained males

  • Damoon Ashtary-Larky (a1) (a2), Alireza Kheirollah (a3), Reza Bagheri (a4), Mohammad Ali Ghaffari (a3), Seyyed Ali Mard (a5), Seyed Jalal Hashemi (a6), Iman Mir (a7) and Alexei Wong (a8)...


Vitamin D deficiency is now a recognised problem affecting multiple physiological functions. The aim of the present study was to evaluate the effect of a single dose of vitamin D3 injection on the inflammatory, muscular damage, metabolic and cardiovascular responses to an acute bout of resistance exercise (RE) in vitamin D-deficient resistance-trained males. Blood samples from fourteen vitamin D-deficient resistance-trained males were obtained during two separate trials: lower vitamin D (LVD) and higher vitamin D (HVD, after vitamin D3 injection). Metabolic, inflammatory, muscle damage and cardiovascular markers were evaluated at baseline, immediately and 1 h after RE. There were significant trial-by-time interactions for insulin and homeostatic model assessment of insulin resistance (HOMA-IR) which significantly (P < 0·05) declined for 1 h after RE in the HVD trial compared with the LVD trial. Homeostasis model assessment of β-cell function (HOMA-β) declines at 1 h post-RE in the HVD trial. There was also a time effect for blood sugar which significantly (P < 0·05) decreased and for creatine kinase, lactate dehydrogenase and IL-6 which increased significantly 1 h post-RE in both trials. There were no significant changes in other inflammatory and cardiovascular markers following both trials. A single injection of vitamin D3 improved insulin resistance and β-cell function following RE in previously vitamin D-deficient resistance-trained males. Conversely, the injection did not change muscle damage and the inflammatory response to acute RE. Intramuscular vitamin D replacement may have key implications for the promotion of glucose metabolism and lowering the risk of diabetes in vitamin D-deficient individuals.


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*Corresponding author: Alexei Wong, email


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1. Larson-Meyer, DE & Willis, KS (2010) Vitamin D and athletes. Curr Sports Med Rep 9, 220226.
2. Holick, MF (2017) The vitamin D deficiency pandemic: approaches for diagnosis, treatment and prevention. Rev Endocr Metab Disord 18, 153165.
3. Baggerly, CA, Cuomo, RE, French, CB, et al. (2015) Sunlight and vitamin D: necessary for public health. J Am Coll Nutr 34, 359365.
4. Guillot, X, Semerano, L, Saidenberg-Kermanac’h, N, et al. (2010) Vitamin D and inflammation. Joint Bone Spine 77, 552557.
5. Willis, KS, Peterson, NJ & Larson-Meyer, DE (2008) Should we be concerned about the vitamin D status of athletes? Int J Sport Nutr Exerc Metab 18, 204224.
6. Miraj, SS, Thunga, G, Kunhikatta, V, et al. (2019) Benefits of vitamin D in sport nutrition. In Nutrition and Enhanced Sports Performance, pp. 497508 [D Bagchi, S Nair and C Sen, editors]. London: Elsevier.
7. Garcia, LA, Ferrini, MG, Norris, KC, et al. (2013) 1,25(OH)2 vitamin D3 enhances myogenic differentiation by modulating the expression of key angiogenic growth factors and angiogenic inhibitors in C2C12 skeletal muscle cells. J Steroid Biochem Mol Biol 133, 111.
8. Sinha, A, Hollingsworth, KG, Ball, S, et al. (2013) Improving the vitamin D status of vitamin D deficient adults is associated with improved mitochondrial oxidative function in skeletal muscle. J Clin Endocrinol Metab 98, E509E513.
9. Henriksen, EJ (2002) Invited review: effects of acute exercise and exercise training on insulin resistance. J Appl Physiol 93, 788796.
10. Ghafourian, M, Ashtary-Larky, D, Chinipardaz, R, et al. (2016) Inflammatory biomarkers’ response to two different intensities of a single bout exercise among soccer players. Iranian Red Crescent Med J 18, e21498.
11. Bruunsgaard, H, Galbo, H, Halkjaer-Kristensen, J, et al. (1997) Exercise-induced increase in serum interleukin-6 in humans is related to muscle damage. J Physiol 499, 833841.
12. Petersen, AMW & Pedersen, BK (2005) The anti-inflammatory effect of exercise. J Appl Physiol 98, 11541162.
13. Choi, M, Park, H, Cho, S, et al. (2013) Vitamin D3 supplementation modulates inflammatory responses from the muscle damage induced by high-intensity exercise in SD rats. Cytokine 63, 2735.
14. Houmard, JA, Tanner, CJ, Slentz, CA, et al. (2004) Effect of the volume and intensity of exercise training on insulin sensitivity. J Appl Physiol 96, 101106.
15. Lloyd, JW, Evans, KA, Zerfass, KM, et al. (2016) Effect of an acute bout of aerobic exercise on chemerin levels in obese adults. Diabetes Metab Syndrome Clin Res Rev 10, 3742.
16. Wylon, K, Drozdenko, G, Krannich, A, et al. (2017) Pharmacokinetic evaluation of a single intramuscular high dose versus an oral long-term supplementation of cholecalciferol. PLOS ONE 12, e0169620.
17. Miranda, H, Fleck, SJ, Simão, R, et al. (2007) Effect of two different rest period lengths on the number of repetitions performed during resistance training. J Strength Cond Res 21, 10321036.
18. De Souza, JC, Tibana, RA, Cavaglieri, CR, et al. (2013) Resistance exercise leading to failure versus not to failure: effects on cardiovascular control. BMC Cardiovasc Disord 13, 105.
19. George, P, Pearson, E & Witham, M (2012) Effect of vitamin D supplementation on glycaemic control and insulin resistance: a systematic review and meta-analysis. Diabetic Med 29, e142e150.
20. Ghanavati, M, Behrooz, M, Rashidkhani, B, et al. (2015) Healthy eating index in patients with cataract: a case–control study. Iranian Red Crescent Med J 17, e22490.
21. Ghaffari, M-A, Payami, S-A, Payami, S-P, et al. (2016) Evaluation of insulin resistance indices in type 2 diabetic patients treated with different anti-diabetic drugs. Open J Endocr Metab Dis 6, 95.
22. Matthews, D, Hosker, J, Rudenski, A, et al. (1985) Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28, 412419.
23. Faul, F, Erdfelder, E, Lang, A-G, et al. (2007) G* Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behavior Res Methods 39, 175191.
24. Kazemi, F (2016) The correlation of resistance exercise-induced myostatin with insulin resistance and plasma cytokines in healthy young men. J Endocrinol Invest 39, 383388.
25. Richter, EA & Hargreaves, M (2013) Exercise, GLUT4, and skeletal muscle glucose uptake. Physiol Rev 93, 9931017.
26. Bordenave, S, Brandou, F, Manetta, J, et al. (2008) Effects of acute exercise on insulin sensitivity, glucose effectiveness and disposition index in type 2 diabetic patients. Diabetes Metab 34, 250257.
27. Koopman, R, Manders, RJ, Zorenc, AH, et al. (2005) A single session of resistance exercise enhances insulin sensitivity for at least 24 h in healthy men. Eur J Appl Physiol 94, 180187.
28. Magkos, F, Tsekouras, Y, Kavouras, SA, et al. (2008) Improved insulin sensitivity after a single bout of exercise is curvilinearly related to exercise energy expenditure. Clin Sci 114, 5964.
29. Henriksen, EJ, Bourey, RE, Rodnick, KJ, et al. (1990) Glucose transporter protein content and glucose transport capacity in rat skeletal muscles. Am J Physiol-Endocrinol Metab 259, E593E598.
30. Stockton, K, Mengersen, K, Paratz, JD, et al. (2011) Effect of vitamin D supplementation on muscle strength: a systematic review and meta-analysis. Osteoporos Int 22, 859871.
31. Teegarden, D & Donkin, SS (2009) Vitamin D: emerging new roles in insulin sensitivity. Nutr Res Rev 22, 8292.
32. Noakes, TD (1987) Effect of exercise on serum enzyme activities in humans. Sports Med 4, 245267.
33. Priest, JB, Oei, TO & Moorehead, WR (1982) Exercise-induced changes in common laboratory tests. Am J Clin Pathol 77, 285289.
34. Brancaccio, P, Maffulli, N & Limongelli, FM (2007) Creatine kinase monitoring in sport medicine. Br Med Bull 81, 209230.
35. Rodrigues, BM, Dantas, E, de Salles, BF, et al. (2010) Creatine kinase and lactate dehydrogenase responses after upper-body resistance exercise with different rest intervals. J. Strength Cond Res 24, 16571662.
36. Güzel, NA, Hazar, S, Erbas, D (2007) Effects of different resistance exercise protocols on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. J Sports Sci Med 6, 417.
37. Bischoff, H, Borchers, M, Gudat, F, et al. (2001) In situ detection of 1, 25-dihydroxyvitamin D receptor in human skeletal muscle tissue. Histochem J 33, 1924.
38. Bracken, MB (2009) Why animal studies are often poor predictors of human reactions to exposure. J Royal Soc Med 102, 120122.
39. Phillips, MD, Mitchell, JB, Currie-Elolf, LM, et al. (2010) Influence of commonly employed resistance exercise protocols on circulating IL-6 and indices of insulin sensitivity. J Strength Cond Res 24, 10911101.
40. Buford, TW, Cooke, MB & Willoughby, DS (2009) Resistance exercise-induced changes of inflammatory gene expression within human skeletal muscle. Eur J Appl Physiol 107, 463.
41. Pedersen, BK, Steensberg, A & Schjerling, P (2001) Muscle-derived interleukin-6: possible biological effects. J Physiol 536, 329337.
42. El-Fakhri, N, McDevitt, H, Shaikh, M, et al. (2014) Vitamin D and its effects on glucose homeostasis, cardiovascular function and immune function. Horm Res Paediatr 81, 363378.
43. Hussin, AM, Ashor, AW, Schoenmakers, I, et al. (2017) Effects of vitamin D supplementation on endothelial function: a systematic review and meta-analysis of randomised clinical trials. Eur J Nutr 56, 10951104.
44. Agbalalah, T, Hughes, SF, Freeborn, EJ, et al. (2017) Impact of vitamin D supplementation on endothelial and inflammatory markers in adults: a systematic review. J Steroid Biochem Mol Biol 173, 292300.
45. Hovsepian, S, Amini, M, Aminorroaya, A, et al. (2011) Prevalence of vitamin D deficiency among adult population of Isfahan City, Iran. J Health Popul Nutr 29, 149.
46. Shahla, A, Charehsaz, S, Talebi, R, et al. (2005) Vitamin D deficiency in young females with musculoskeletal complaints in Urmia, northwest of Iran. Iranian J Med Sci 30, 8890.
47. Rahnavard, Z, Eybpoosh, S, Homami, MR, et al. (2010) Vitamin D deficiency in healthy male population: results of the Iranian multi-center osteoporosis study. Iran J Public Health 39, 45.
48. Gaafar, M & Badr, S (2013) An alarming high prevalence of vitamin D deficiency among healthy adults. Life Sci J 10, 32923298.
49. Ardawi, M-S, Sibiany, A, Bakhsh, T, et al. (2012) High prevalence of vitamin D deficiency among healthy Saudi Arabian men: relationship to bone mineral density, parathyroid hormone, bone turnover markers, and lifestyle factors. Osteoporos Int 23, 675686.
50. Salek, M, Hashemipour, M, Aminorroaya, A, et al. (2008) Vitamin D deficiency among pregnant women and their newborns in Isfahan, Iran. Exp Clin Endocrinol Diabetes 116, 352356.
51. Ziaee, A, Javadi, A, Javadi, M, et al. (2013) Nutritional status assessment of Minodar residence in Qazvin City, Iran: vitamin D deficiency in sunshine country, a public health issue. Glob J Health Sci 5, 174.


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A single injection of vitamin D3 improves insulin sensitivity and β-cell function but not muscle damage or the inflammatory and cardiovascular responses to an acute bout of resistance exercise in vitamin D-deficient resistance-trained males

  • Damoon Ashtary-Larky (a1) (a2), Alireza Kheirollah (a3), Reza Bagheri (a4), Mohammad Ali Ghaffari (a3), Seyyed Ali Mard (a5), Seyed Jalal Hashemi (a6), Iman Mir (a7) and Alexei Wong (a8)...


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