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Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats

  • Hiroshi Watanabe (a1), Kei Sonoyama (a1), Jun Watanabe (a1), Natsu Yamaguchi (a1), Hiroto Kikuchi (a2), Taizo Nagura (a2), Tsutomu Aritsuka (a2), Kyoko Fukumoto (a1) and Takanori Kasai (a1)...

Abstract

Oral administration of raffinose, a naturally occurring indigestible oligosaccharide, has reportedly ameliorated atopic dermatitis in human subjects although the mechanism is unknown. The present study investigated the effect of dietary raffinose on allergen-induced airway eosinophilia in ovalbumin-sensitised Brown Norway rats as an atopic disease model. Brown Norway rats were immunised by subcutaneous injection with ovalbumin on day 0 and fed either a control diet or the diet supplemented with raffinose (50 g/kg diet). The rats were exposed to aerosolised ovalbumin on day 20, and broncho-alveolar lavage fluid was obtained on the next day. The number of eosinophils in the fluid was significantly lower in the rats fed the raffinose diet than in those fed the control diet. Dietary raffinose significantly reduced IL-4 and IL-5 mRNA levels in lung tissue and tended to lower ovalbumin-specific Ig E levels. Suppression of eosinophilia by dietary raffinose was still observed in caecectomised and neomycin-administered rats, suggesting little contribution by the colonic bacteria to the effect of raffinose. Intraperitoneal administration of raffinose also suppressed eosinophilia. Significant concentrations of raffinose were detected in portal venous and abdominal arterial plasma after the intragastric administration of raffinose. Overall, the findings suggest that dietary raffinose ameliorates allergic airway eosinophilia at least partly via post-absorptive mechanisms in Brown Norway rats.

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Corresponding author

*Corresponding author: fax +81 11 706 2496, email ksnym@chem.agr.hokudai.ac.jp

References

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Benno, Y, Endo, K, Shiragami, N, Sayama, K&Mitsuoka, TEffects of raffinose intake on human fecal microflora Bifid Microflora (1987) 6 5963
Coffman, RL, Ohara, J, Bond, MW,Carty, J, Zlotnik, A&Paul, WEB cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells J Immunol (1986) 136 45384541
Coyle, AJ, Le, Gros, Bertrand, C,Tsuyuki, S, Heusser, CH, Kopf, M&Anderson, GPInterleukin-4 is required for the induction of lung Th2 mucosal immunity Am J Respir Cell Mol Biol (1995) 13 5459
Del Prete, G, Maggi, E, Parronchi, P,Chretien, I, Tiri, A, Macchia, D, Ricci, M, Banchereau, J,De Vries, J&Romagnani, SIL-4 is an essential factor for the IgE synthesis induced in vitro by human T cell clones and their supernatants J Immunol (1988) 140 41934198
Elwood, W, Lotvall, JO, Barnes, PJ&Chung, KFCharacterization of allergen-induced bronchial hyperresponsiveness and airway inflammation in actively sensitized brown-Norway rats J Allergy Clin Immunol (1991) 88 951960
Fujisaki, H, Nagura, T, Kawamoto, T&Sayama, KThe effects of raffinose administration on the fecal flora, organic acids and putrefactive products in humans (in Japanese) Bifidus (1994) 8 15
Gracie, JA&Bradley, JAInterleukin-12 induces interferon-γ-dependent switching of IgG alloantibody subclass Eur J Immunol (1996) 26 12171221
Haczku, A, Macary, P, Haddad, EB,Huang, TJ, Kemeny, DM,Moqbel, R&Chung, KFExpression of Th-2 cytokines interleukin-4 and -5 and of Th-1 cytokine interferon-γ in ovalbumin-exposed sensitized Brown-Norway rats Immunology (1996) 88 247251
Holt, PG&Turner, KJPersistent IgE-secreting cells which are refractory to T-cell control Int Arch Allergy Appl Immunol (1985) 77 4546
Isolauri, E, Arvola, T, Sütas, Y, Moilanen, E&Salminen, SProbiotics in the management of atopic eczema Clin Exp Allergy (2000) 30 16041610
Iwamoto, I, Nakajima, H, Endo, H&Yoshida, SInterferon γ regulates antigen-induced eosinophil recruitment into the mouse airways by inhibiting the infiltration of CD4 + T cells J Exp Med (1993) 177 573576
Kalliomäki, M, Salminen, S, Arvilommi, H, Kero, P, Koskinen, P&Isolauri, EProbiotics in primary prevention of atopic disease: a randomised placebo-controlled trial Lancet (2001) 357 10761079
Kato, I, Endo-Tanaka, K&Yokokura, TSuppressive effects of the oral administration of Lactobacillus casei on type II collagen-induced arthritis in DBA/1 mice Life Sci (1998) 63 635644
Leung, DYM&Bieber, TAtopic dermatitis Lancet (2003) 361 151160
Majamaa, H&Isolauri, EProbiotics: a novel approach in the management of food allergy J Allergy Clin Immunol (1997) 99 179185
Matsuda, M, Takeuchi, S&Nagura, TInfluence of raffinose for atopic dermatitis (in Japanese) Allergy Practice (1998) 18 10921095
Mitsuoka, T, Sega, T&Yamamoto, SEine verbesserte methodik der qualitativen und quantitativen analyse der darmflora von menschen und tieren (Improved methodology for qualitative and quantitative analysis of the intestinal flora of man and animals) Zbl Bakt Hyg I Abt Orig (1965) 195 455469
Mosmann, TR, Cherwinski, H, Bond, MW, Giedlin, MA&Coffman, RLTwo types of murine helper T cell clone. I Definition according to profiles of lymphokine activities and secreted proteins J Immunol (1986) 136 23482357
Nagura, T, Hachimura, S, Hashiguchi, M, Ueda, Y, Kanno, T, Kikuchi, H, Sayama, K&Kaminogawa, SSuppressive effect of dietary raffinose on T-helper 2 cell-mediated immunity Br J Nutr (2002) 88 421426
Nagura, T, Muraguchi, H, Uchino, K,Aritsuka, T,&Benno, YEffects of ingestion of raffinose-rich soup on the fecal flora and daily defecation in humans (in Japanese). J Intest Microbiol (1999) 13 17
Nakajima, H, Iwamoto, I, Tomoe, S,Matsumura, R, Tomioka, H, Takatsu, K&Yoshida, SCD4 + T-lymphocytes and interleukin-5 mediate antigen-induced eosinophil infiltration into the mouse trachea Am Rev Respir Dis (1992) 146 374377
Niewoehner, CB, Gilboe, DP&Nuttall, FQMetabolic effects of oral glucose in the liver of fasted rats Am J Physiol (1984 a) 246 E89E94
Niewoehner, CB, Gilboe, DP, Nuttall, GA&Nuttall, FQMetabolic effects of oral fructose in the liver of fasted rats Am J Physiol (1984 b) 247 E505E512
Niewoehner, CB, Neil, B&Martin, THepatic uptake and metabolism of oral galactose in adult fasted rats Am J Physiol (1990) 259 E804E813
Rautava, S, Kalliomäki, M&Isolauri, EProbiotics during pregnancy and breast-feeding might confer immunomodulatory protection against atopic disease in the infant J Allergy Clin Immunol (1990) 109 119121
Reeves, PG, Nielsen, FH&Fahey, GC Jr AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet J Nutr (1993) 123 19391951
Sayama, K, Kamada, T, Oikawa, S&Masuda, TProduction of raffinose: a new byproduct of the beet sugar industry Zuckerindustry (1992) 117 893898
Schley, PD&Field, CJThe immune-enhancing effects of dietary fibres and prebiotics Br J Nutr (2002) 87Suppl. 2 S221S230
Sher, A&Coffman, RLRegulation of immunity to parasites by T cells and T cell-derived cytokines Annu Rev Immunol (1992) 10 385409
Sonoyama, K, Kiriyama, S&Niki, RExpression of apolipoprotein A-I mRNA in liver and intestine of cecectomized rats fed beet fiber J Nutr Biochem (1995) 6 380384
Sonoyama, K, Rutatip, S&Kasai, TGene expression of activin, activin receptors, and follistatin in intestinal epithelial cells Am J Physiol (2000) 278 G89G97
Watanabe, J, Fukumoto, K, Fukushi, E, Sonoyama, K&Kawabata, JIsolation of tryptophan as an inhibitor of ovalbumin permeation and analysis of its suppressive effect on oral sensitization Biosci Biotechnol Biochem (2004) 68 5965
Watanabe, J, Tanabe, S, Watanabe, M, Kasai, T, &Sonoyama, KConsumption of hypoallergenic flour prevents gluten-induced airway inflammation in Brown Norway rats Biosci Biotechnol Biochem (2001) 65 17291735

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