Skip to main content Accessibility help
×
Home

Raised saturated-fat intake worsens vascular function in virgin and pregnant offspring of streptozotocin-diabetic rats

  • Kathleen Holemans (a1), Robert Gerber (a2), Ivan O'Brien-Coker (a3), Anthony Mallet (a3), Rieta Van Bree (a1), F. André Van Assche (a1) and Lucilla Poston (a2)...

Abstract

Adult offspring of severely diabetic pregnant rats are insulin resistant and display cardiovascular dysfunction. When pregnant they develop mild hyperglycaemia. Diets high in saturated fat have been implicated in the development of cardiovascular disease and vascular dysfunction. In the present study we have determined vascular function in small mesenteric arteries from offspring of normal (OC) and diabetic (OD) rats fed standard chow and offspring of diabetic rats fed a diet high in saturated fats (OD-HF) from weaning to adulthood, and throughout their subsequent pregnancies. OD rats displayed an increased sensitivity to noradrenaline (P < 0·05) and impaired sensitivity to the endothelium-dependent vasodilator, acetylcholine. The component of acetylcholine-induced relaxation attributable to endothelium-derived hyperpolarizing factor was reduced in OD-HF rats. Pregnant OD rats also demonstrated impaired maximum relaxation to acetylcholine (pregnant OD rats v. pregnant OC rats P < 0·05). In pregnant OD-HF rats noradrenaline sensitivity was enhanced and endothelium-dependent relaxation further reduced (pregnant OD-HF rats v. pregnant OC rats P < 0·001). The isoprostane, 8-epi-prostaglandin F, a marker of oxidative stress, was increased in pregnant OD rats (pregnant OD rats v. pregnant OC rats P < 0·001) and further increased in pregnant OD-HF rats (pregnant OD-HF rats v. pregnant OD rats P < 0·05). We conclude that a high-saturated-fat diet leads to deterioration in specific components of vascular function in OD rats. When pregnant, vascular function of OD-HF rats is further compromised. Pregnancy in the OD rats is associated with a striking increase in a marker of oxidative stress, which increases further if the saturated fat intake is raised.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Raised saturated-fat intake worsens vascular function in virgin and pregnant offspring of streptozotocin-diabetic rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Raised saturated-fat intake worsens vascular function in virgin and pregnant offspring of streptozotocin-diabetic rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Raised saturated-fat intake worsens vascular function in virgin and pregnant offspring of streptozotocin-diabetic rats
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr Kathleen Holemans, fax +32 16 344205, email Kathleen.Holemans@uz.kuleuven.ac.be

References

Hide All
Aerts, L and Van Assche, FA (1979) Is gestational diabetes an acquired condition?. Journal of Developmental Physiology 1, 219225.
Bauersachs, J, Rudiger, P, Hecker, M, Sauer, E, Fleming, I and Buse, R (1996) Nitric oxide attenuates the release of endothelium-derived hyperpolarizing factor. Circulation 94, 33413347.
Chowienczyk, PJ, Brett, SE, Gopaul, NK, Dhir, S, Meeking, D, Marchetti, M, Russell-Jones, DR, Anggard, EE and Ritter, JM (1998) Oral administration of a novel water soluble antioxidant reduces oxidative stress and improves endothelial function in non-insulin-dependent diabetes. Circulation 98(Suppl.), I175.
Chowiensczyk, PJ and Watts, GF (1997) Endothelial dysfunction, insulin resistance and non-insulin-dependent diabetes. Endocrinology and Metabolism 4, 225232.
Cooke, JP and Dzau, VJ (1997) Nitric oxide synthase: Role in the genesis of vascular disease. Annual Review of Medicine 48, 489509.
Erhardt, JG, Lim, SS and Bode, C (1997) A diet rich in fat and poor in dietary fiber increases the in vitro formation of reactive oxygen species in human faeces. Journal of Nutrition 127, 706709.
Gauguier, D, Bihoreau, MT, Ktorza, A, Berthault, MF and Picon, L (1990) Inheritance of diabetes mellitus as consequence of gestational hyperglycemia in rats. Diabetes 39, 734739.
Gerber, RT, Anwar, MA and Poston, L (1998) Enhanced acetylcholine induced relaxation in small mesenteric arteries from pregnant rats: an important role for endothelium-derived hyperpolarizing factor (EDHF). British Journal of Pharmacology 125, 455460.
Gerber, RT, Holemans, K, O'Brien-Coker I, Mallet, A, Poston, L and Van Assche, FA (1999) Is increased oxidative stress in the pregnant streptozotocin-diabetic rat involved in fetal 'programming'?. Journal of the Society for Gynecological Investigations 6, 50A.
Gerber, RT, Holemans, K, O'Brien-Coker, I, Mallet, A, van Bree, R, Van Assche, FA and Poston, L (1999) Cholesterol independent endothelial dysfunction in virgin and pregnant rats fed a high saturated fat diet. Journal of Physiology 517, 607616.
Grundlegger, ML and Thenen, SW (1982) Decreased insulin binding, glucose transport, and glucose metabolism in soleus muscle of rats fed a high-fat diet. Diabetes 31, 232237.
Halliwell, B (1996) Free radicals, proteins and DNA: oxidative damage versus redox regulation. Biochemical Society Transactions 24, 10231027.
Holemans, K, Aerts, L and Van Assche, FA (1991) Evidence for an insulin resistance in the adult offspring of pregnant streptozotocin-diabetic rats. Diabetologia 34, 8185.
Holemans, K, Aerts, L and Van Assche, FA (1991) Absence of pregnancy induced alterations in tissue insulin sensitivity in the offspring of diabetic rats. Journal of Endocrinology 131, 387393.
Holemans, K, Gerber, RT, Meurrens, K, De Clerck, F, Poston, L and Van Assche, FA (1999) Streptozotocin-diabetes in the pregnant rat induces cardiovascular dysfunction in adult offspring. Diabetologia 42, 8189.
Holemans, K, Van Bree, R, Verhaeghe, J, Aerts, L and Van Assche, FA (1993) In vivo glucose utilization by individual tissues in virgin and pregnant offspring of severely diabetic rats. Diabetes 42, 530536.
Holemans, K, Van Bree, R, Verhaeghe, J, Meurrens, K and Van Assche, FA (1997) Maternal semistarvation and streptozotocin-diabetes in rats have different effects on the in vivo glucose uptake by peripheral tissues in their female adult offspring. Journal of Nutrition 127, 13711376.
Hunt, JV, Dean, RT and Wolff, SP (1988) Hydroxyl radical production and autoxidative glycosylation: glucose autoxidation as the cause of protein damage in the experimental glycation model of diabetes mellitus and ageing. Biochemical Journal 256, 205212.
Ibrahim, W, Lee, U, Yeh, C, Szabo, J, Bruckner, G and Chow, CK (1997) Oxidative stress and antioxidant status in mouse liver: effects of dietary lipid, vitamin E and iron. Journal of Nutrition 127, 14011406.
Knowler, WC, Pettitt, DJ, Saad, MF, Charles, MA, Nelson, RG, Howard, BV, Bogardus, C and Bennett, PH (1991) Obesity in the Pima Indians: Its magnitude and relationship to diabetes. American Journal of Clinical Nutrition 53, 1543S1551S.
Morrow, JD and Roberts, LJ (1996) The isoprostanes. Current knowledge and directions for future research. Biochemical Pharmacology 51, 19.
Mulvany, MJ and Halpern, W (1977) Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circulation Research 41, 1926.
Nourooz-Zadeh, J, Gopaul, NK, Barrow, S, Mallet, AI and Anggard, EE (1996) Analysis of F2-isoprostanes as indicators of non-enzymatic lipid peroxidation in vivo by gas chromatography-mass spectometry: development of a solid-phase extraction procedure. Journal of Chromatography B 667, 199208.
O'Brian, SF, McKendrick, JP, Radomski, MW, Davidge, ST and Russell, JC (1998) Vascular wall reactivity in conductance and resistance arteries: differential effects of insulin resistance. Canadian Journal of Physiology and Pharmacology 76, 7276.
O'Dea, K, Traianedes, K, Ireland, P, Niall, M, Sadler, J, Hopper, J and De Luise, M (1989) The effects of diets differing in fat carbohydrate and fiber on carbohydrate and lipid metabolism in type 2 diabetes. Journal of the American Dietetic Association 89, 10761089.
Oh, W, Geraldi, NL and Cha, CJ (1991) The cross-generation effect of neonatal macrosomia in rat pups of streptozotocin-induced diabetes. Pediatric Research 29, 606610.
Palmer, AM, Thomas, CR, Gopaul, N, Dhir, S, Anggard, EE, Poston, L and Tribe, RM (1998) Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocin-diabetic rat. Diabetologia 41, 148156.
Pascoe, WS and Storlien, LH (1990) Inducement by fat feeding of basal hyperglycemia in rats with abnormal B-cell function. Model for study of etiology and pathogenesis of NIDDM. Diabetes 39, 226233.
Pettitt, DJ, Aleck, KA, Baird, HR, Carraher, MJ, Bennett, PH and Knowler, WC (1988) Congenital susceptibility to NIDDM. Role of the intrauterine environment. Diabetes 37, 622628.
Phillips, DI, Barker, DJ, Hales, CN, Hirst, S and Osmond, C (1994) Thinness at birth and insulin resistance in adult life. Diabetologia 37, 150154.
Poston, L (1996) Maternal vascular function in pregnancy. Journal of Human Hypertension 10, 391394.
Rasmussen, KM and Warman, NL (1983) Effect of maternal malnutrition during the reproductive cycle on growth and nutritional status of suckling rat pups. American Journal of Clinical Nutrition 38, 7783.
Reaven, GM (1988) Role of insulin resistance in human disease. Diabetes 37, 15951607.
Rosenthal, M, Haskell, WL, Solomon, R, Widstrom, A and Reaven, GM (1983) Demonstration of a relationship between level of physical training and insulin-stimulated glucose utilization in normal humans. Diabetes 32, 408411.
Rosso, P and Kava, R (1980) Effects of food restriction on cardiac ouput and blood flow to the uterus and placenta in the pregnant rat. Journal of Nutrition 110, 23502354.
Ryan, EA, Liu, D, Bell, RC, Finegood, DT and Crawford, J (1995) Long term consequences in offspring of diabetes in pregnancy: studies with syngeneic islet transplanted streptozotocin-diabetic rats. Endocrinology 136, 55875592.
Salter, AM, Hayashi, R, Al-Seeni, M, Brown, NF, Sorenson, O, Atkinson, EA, Middleton, B, Bleakly, RC and Brindley, DN (1991) Effects of hypothyroidism and high fat feeding on mRNA concentrations for the low-density lipoprotein receptor and on acyl-CoA: cholesterol acethyltransferase activities in rat liver. Biochemical Journal 276, 825832.
Silverman, BL, Metzger, BE, Cho, NH and Loeb, CA (1995) Impaired glucose tolerance in adolescent offspring of diabetic mothers. Diabetes Care 18, 611617.
Siman, CM and Eriksson, UJ (1997) Vitamin E decreases the occurrence of malformations in the offspring of diabetic rats. Diabetes 46, 10541061.
Storlien, LH, James, DE, Burleigh, KM, Chisholm, DJ and Kraegen, EW (1986) Fat feeding causes widespread in vivo insulin resistance, decreased energy expenditure, and obesity in rats. American Journal of Physiology 251, E576E583.
Storlien, LH, Jenkins, AB, Chisholm, DJ, Pascoe, WS, Khouri, S and Kraegen, EW (1991) Influence of dietary fat composition on development of insulin resistance in rats. Relationship to muscle triglyceride and w-3 fatty acids in muscle phospholipid. Diabetes 40, 280289.
Taylor, R, Badcock, J, King, H, Pargeter, K, Zimmet, P, Fred, T, Lund, M, Ringrose, M, Bach, F, Wang, RL and Sladder, T (1992) Dietary intake, exercise, obesity and noncommunicable disease in rural and urban populations of three Pacific Islands countries. Journal of the American College of Nutrition 11, 283293.
Taylor, PD, Graves, JE and Poston, L (1995) Selective impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated resistance arteries of the streptozotocin-induced diabetic rat. Clinical Science 88, 519524.
Wisdom, SJ, Wilson, R, McKillop JH and Walker, JJ (1991) Antioxidant systems in normal pregnancy and in pregnancy induced hypertension. American Journal of Obstetrics and Gynecology 165, 17011704.

Keywords

Raised saturated-fat intake worsens vascular function in virgin and pregnant offspring of streptozotocin-diabetic rats

  • Kathleen Holemans (a1), Robert Gerber (a2), Ivan O'Brien-Coker (a3), Anthony Mallet (a3), Rieta Van Bree (a1), F. André Van Assche (a1) and Lucilla Poston (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed