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Plasma response to a single dose of dietary β-cryptoxanthin esters from papaya (Carica papaya L.) or non-esterified β-cryptoxanthin in adult human subjects: a comparative study

Published online by Cambridge University Press:  09 March 2007

Dietmar E. Breithaupt*
Affiliation:
Institute of Food Chemistry, University of Hohenheim, Garbenstr. 28, 70593 Stuttgart, Germany
Philipp Weller
Affiliation:
Institute of Food Chemistry, University of Hohenheim, Garbenstr. 28, 70593 Stuttgart, Germany
Maike Wolters
Affiliation:
Institute of Food Science, University of Hannover, Wunstorferstr. 14, 30453 Hannover, Germany
Andreas Hahn
Affiliation:
Institute of Food Science, University of Hannover, Wunstorferstr. 14, 30453 Hannover, Germany
*
*Corresponding author: Dr Dietmar E. Breithaupt, fax +49 711 4594096, email breithau@uni-hohenheim.de
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Abstract

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Many orange-coloured fruits contain β-cryptoxanthin in its non-esterified as well as its esterified form. Information concerning the absorption of β-cryptoxanthin, especially with regard to the metabolism of its fatty acid esters, is rather scarce. The present study assessed the plasma concentration reached after consumption of a single dose of native β-cryptoxanthin esters from papaya (Carica papaya L.) or non-esterified β-cryptoxanthin in equal total amounts. In a randomized, single-blind crossover study, twelve subjects were served a portion of yoghurt containing esterified or non-esterified β-cryptoxanthin (1.3 mg absolute) together with a balanced breakfast. Between the two intervention days, there was a 2-week depletion period. After a fasting blood sample had been taken, futher samples were taken from the subjects at 3, 6, 9, 12 and 24 h. The concentration of non-esterified β-cryptoxanthin in the whole plasma was determined by HPLC; β-cryptoxanthin identification was confirmed by liquid chromatography–atmospheric pressure chemical ionization–MS analyses. Irrespective of the consumed diet, the plasma β-cryptoxanthin concentrations increased significantly (P=0·05) and peaked after 6–12 h. The concentration curves, as well as the areas under the curves, were not distinguishable according to two-sided F and t tests (P=0·05). Standardization of β-cryptoxanthin concentrations to plasma triacylglycerol and cholesterol had no impact on the results. Thus, the present study indicates comparable bioavailability of both non-esterified β-cryptoxanthin and mixtures of β-cryptoxanthin esters. The results support the existence of an effective enzymatic cleavage system accepting various β-cryptoxanthin esters.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2003

References

Barua, AB (1999) Intestinal absorption of epoxy-β-carotenes by humans. Biochem J 339, 359362.CrossRefGoogle ScholarPubMed
Bieri, JG, Brown, ED & Smith, JC Jr (1985) Determination of individual carotenoids in human plasma by high performance liquid chromatography. J Liq Chromatogr 8, 473484.CrossRefGoogle Scholar
Böhm, V & Bitsch, R (1999) Intestinal absorption of lycopene from different matrices and interactions to other carotenoids, the lipid status and the antioxidant capacity of human plasma. Eur J Nutr 38, 118125.Google ScholarPubMed
Breithaupt, DE (2000) Enzymatic hydrolysis of carotenoid fatty acid esters of red pepper (Capsicum annuum L.) by a lipase from Candida rugosa. Z Naturforsch 55C, 971975.CrossRefGoogle Scholar
Breithaupt, DE & Bamedi, A (2001) Carotenoid esters in vegetables and fruits: a screening with emphasis on β-crypto-xanthin esters. J Agric Food Chem 49, 20642070.CrossRefGoogle ScholarPubMed
Breithaupt, DE, Bamedi, A & Wirt, U (2002) Carotenol fatty acid esters: easy substrates for digestive enzymes? Comp Biochem Physiol B 134, 721728.CrossRefGoogle Scholar
Breithaupt, DE & Schwack, W (2000) Determination of free and bound carotenoids in paprika (Capsicum annuum L.) by LC/ MS. Eur Food Res Technol 211, 5255.CrossRefGoogle Scholar
Gärtner, C, Stahl, W & Sies, H (1997) Lycopene is more bioavailable from tomato paste than from fresh tomatoes. Am J Clin Nutr 66, 116122.CrossRefGoogle ScholarPubMed
Goswami, UC (1984) Metabolism of cryptoxanthin in freshwater fish. Br J Nutr 52, 575581.CrossRefGoogle ScholarPubMed
Grobusch-Klipstein, K, Launer, LJ, Geleijnse, JM, Boeing, H, Hofman, A & Witteman, JCM (2000) Serum carotenoids and atherosclerosis: the Rotterdam study. Atherosclerosis 148, 4956.CrossRefGoogle Scholar
Ito, Y, Ochiai, J, Sasaki, R, et al. (1990) Serum concentrations of carotenoids, retinol, and α-tocopherol in healthy persons determined by high-performance liquid chromatography. Clin Chim Acta 194, 131144.CrossRefGoogle ScholarPubMed
John, J, Kishore, GS, Subbarayan, C & Cama, HR (1970) Metabolism and biological potency of cryptoxanthin in rat. Indian J Biochem 7, 222225.Google Scholar
Jordan, P, Brubacher, D, Moser, U, Stähelin, HB & Gey, KF (1995) Vitamin E and vitamin A concentrations in plasma adjusted for cholesterol and triacylglycerides by multiple regression. Clin Chem 41, 924927.CrossRefGoogle Scholar
Khachik, F, Beecher, GR & Goli, MB (1992) Separation and identification of carotenoids and their oxidation products in the extracts of human plasma. Anal Chem 64, 21112122.CrossRefGoogle ScholarPubMed
Khachik, F, Spangler, CJ & Smith, Jr JC (1997) Identification, quantification, and relative concentrations of carotenoids and their metabolites in human milk and serum. Anal Chem 69, 18731881.CrossRefGoogle ScholarPubMed
Krinsky, NI, Russett, MD, Handelman, J & Snodderly, M (1990) Structural and geometrical isomers of carotenoids in human plasma. J Nutr 120, 16541662.CrossRefGoogle ScholarPubMed
Olmedilla, R, Granado, F, Gil-Martinez, E, Blanco, I & Rojas-Hidalgo, E (1997) Reference values for retinol, tocopherol, and main carotenoids in serum of control and insulin-dependent diabetic Spanish subjects. Clin Chem 43, 10661071.CrossRefGoogle ScholarPubMed
Olmedilla, B, Granado, F, Southon, S, et al. (2001) Serum concentrations of carotenoids and vitamins A, E, and C in control subjects from five European countries. Br J Nutr 85, 227238.CrossRefGoogle Scholar
Olson, JA (1994) Absorption, transport, and metabolism of carotenoids in humans. Pure Appl Chem 66, 10111016.CrossRefGoogle Scholar
Rock, CL (1997) Carotenoids: biology and treatment. Pharmacol Ther 75, 185197.CrossRefGoogle ScholarPubMed
Ross, MA, Crosley, LK, Brown, KM, et al. (1995) Plasma concentrations of carotenoids and antioxidant vitamins in Scottish males: influences of smoking. Eur J Clin Nutr 49, 861865.Google ScholarPubMed
Scherz, H, Senser, F & Souci, SW (2000) Souci–Fachmann–Kraut: Die Zusammensetzung der Lebensmittel: Nährwert-Tabellen (Food Composition and Nutrition Tables), 6th ed. Stuttgart: Medpharm Scientific Publishers, Wissenschaftliche Verlagsgesellschaft mbH.Google Scholar
Stahl, W, Schwarz, W, Sundquist, AR & Sies, H (1992) cis-trans Isomers of lycopene and β-carotene in human serum and tissues. Arch Biochem Biophys 294, 173177.CrossRefGoogle Scholar
Thurnham, DI (1988) Do higher vitamin A requirements in men explain the difference between the sexes in plasma provitamin A carotenoids and retinol? Proc Nutr Soc 47, 181A.Google Scholar
Thurnham, DI (1989) Lutein, cholesterol, and risk of cancer. Lancet 19, 441442.CrossRefGoogle Scholar
Yao, L, Liang, Y, Trahanovsky, WS, Serfass, RE & White, WS (2000) Use of a 13C tracer to quantify the plasma appearance of a physiological dose of lutein in humans. Lipids 35, 339348.CrossRefGoogle ScholarPubMed
van Vliet, T, Schreurs, WHP & van den, Berg H (1995) Intestinal β-carotene absorption and cleavage in men: response of β-carotene and retinyl esters in the triacylglycerol-rich lipoprotein fraction after as single oral dose of β-carotene. Am J Clin Nutr 62, 110116.CrossRefGoogle Scholar
Wingerath, T, Stahl, W & Sies, H (1995) β-Cryptoxanthin selectively increases in human chylomicrons upon ingestion of tangerine concentrate rich in β-cryptoxanthin esters. Arch Biochem Biophys 324, 385390.CrossRefGoogle Scholar