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A novel high-amylose barley cultivar (Hordeum vulgare var. Himalaya 292) lowers plasma cholesterol and alters indices of large-bowel fermentation in pigs

  • Anthony R. Bird (a1), Michelle Jackson (a1), Roger A. King (a1), Debra A. Davies (a1), Sylvia Usher (a1) and David L. Topping (a1)...

Abstract

Hordeum vulgare var. Himalaya 292 is a new barley cultivar with altered starch synthesis and less total starch but more amylose, resistant starch (RS) and total and soluble NSP including β-glucan. To determine its nutritional potential, young pigs were fed diets containing stabilised wholegrain flours from either Himalaya 292, Namoi (a commercial barley), wheat bran or oat bran at equivalent dietary NSP concentrations for 21 d. Serum total cholesterol was significantly lowered by the Himalaya 292 diet relative to wheat bran, indicating that Himalaya 292 retained its hypocholesterolaemic potential. In all groups SCFA concentrations were highest in the proximal colon and decreased towards the rectum. Digesta pH was lowest in the proximal colon and highest in the distal colon. Large-bowel and faecal pH were significantly lower in the pigs fed the barley and oat diets, indicating greater bacterial fermentation. Caecal and proximal colonic pH was lowest and SCFA pools highest in the pigs fed Himalaya 292. Total and individual SCFA were lowest in the mid- and distal colon of the pigs fed Himalaya 292 or oat bran. These data suggest the presence of more RS in Himalaya 292 and suggest that its fermentation was rapid relative to transit. Differences in faecal and large-bowel anaerobic, aerobic, coliform and lactic acid bacteria were relatively small, indicating a lack of a specific prebiotic action. These data support the potential of this novel barley cultivar to improve health through plasma cholesterol reduction and increased large-bowel SCFA production.

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      A novel high-amylose barley cultivar (Hordeum vulgare var. Himalaya 292) lowers plasma cholesterol and alters indices of large-bowel fermentation in pigs
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      A novel high-amylose barley cultivar (Hordeum vulgare var. Himalaya 292) lowers plasma cholesterol and alters indices of large-bowel fermentation in pigs
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Corresponding author

*Corresponding author: Dr A. R. Bird, fax +61 8 8303 8899, email tony.bird@csiro.au

References

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Anderson, JW, Davidson, MH, Blonde, L, Brown, WV, Howard, WJ, Ginsberg, H, Allgood, LD & Weingand, KW (2000) Long-term cholesterol-lowering effects of psyllium as an adjunct to diet therapy in the treatment of hypercholesterolemia. Am J Clin Nutr 71, 14331438.
Asp, N-G (1992) Resistant starch. Eur J Clin Nutr 46, Suppl.. 2, S1.
Bach-Knudsen, KE, Jensen, BB, Andersen, JO & Hansen, I (1991) Gastrointestinal implications in pigs of wheat and oat fractions. 2. Microbial activity in the gastrointestinal tract. Br J Nutr 65, 233248.
Bird, AR, Brown, IL & Topping, DL (2000 a) Starches, resistant starches, the gut microflora and human health. Curr Issues Intest Microbiol 1, 2537.
Bird, AR, Flory, C, Davies, DA, Usher, S & Topping, DL (2004) A novel barley cultivar (Himalaya 292) with a specific gene mutation in starch synthase IIa raises large bowel starch and short chain fatty acids in rats. J Nutr 134, 831835.
Bird, AR, Hayakawa, T, Marsono, Y, Gooden, JM, Record, IR, Correll, RL & Topping, DL (2000 b) Coarse brown rice increases fecal and large bowel short-chain fatty acids and starch but lowers calcium in the large bowel of pigs. J Nutr 130, 17801787.
Black, DM (2003) Therapeutic targets in cardiovascular disease: a case for high-density lipoprotein cholesterol. Am J Cardiol 91, 40E43E.
Borum, ML (2001) Constipation: evaluation and management. Prim Care 28, 577590.
Brown, L, Rosner, B, Willett, WW & Sacks, FM (1999) Cholesterol-lowering effects of dietary fiber: a meta-analysis. Am J Clin Nutr 69, 3042.
Coughlan, BJ & Sorrentino, MJ (2000) Does hypertriglyceridemia increase risk for CAD? Growing evidence suggests it plays a role. Postgrad Med 108, 7784.
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125, 14011412.
Goodlad, JS & Mathers, JC (1990) Large bowel fermentation in rats given diets containing raw peas ( Pisum sativum ). Br J Nutr 64, 569587.
Govers, MJ, Gannon, NJ, Dunshea, FR, Gibson, PR & Muir, JG (1999) Wheat bran affects the site of fermentation of resistant starch and luminal indexes related to colon cancer risk: a study in pigs. Gut 45, 840847.
Jackson, KA, Suter, DA & Topping, DL (1994) Oat bran, barley and malted barley lower plasma cholesterol relative to wheat bran but differ in their effects on liver cholesterol in rats fed diets with and without cholesterol. J Nutr 124, 16781684.
Jenkins, DJ, Kendall, CW & Vuksan, V (2002) Soluble fiber intake at a dose approved by the US Food and Drug Administration for a claim of health benefits: serum lipid risk factors for cardiovascular disease assessed in a randomized controlled crossover trial. Am J Clin Nutr 75, 834839.
Kerckhoffs, DA, Brouns, F, Hornstra, G & Mensink, RP (2002) Effects on the human serum lipoprotein profile of beta-glucan, soy protein and isoflavones, plant sterols and stanols, garlic and tocotrienols. J Nutr 132, 24942505.
Kestin, M, Moss, R, Clifton, PM & Nestel, PJ (1990) Comparative effects of three cereal brans on plasma lipids, blood pressure, and glucose metabolism in mildly hypercholesterolemic men. Am J Clin Nutr 52, 661666.
Lewis, SJ & Heaton, KW (1997) Increasing butyrate concentration in the distal colon by accelerating intestinal transit. Gut 41, 245251.
Lia, A, Hallmans, G, Sandberg, AS, Sundberg, B, Aman, P & Andersson, H (1995) Oat β-glucan increases bile acid excretion and a fiber-rich barley fraction increases cholesterol excretion in ileostomy subjects. Am J Clin Nutr 62, 12451251.
Lupton, JR, Robinson, MC & Morin, JL (1994) Cholesterol-lowering effect of barley bran flour and oil. J Am Diet Assoc 94, 6570.
McIntosh, GH, Whyte, J, McArthur, R & Nestel, PJ (1991) Barley and wheat foods: influence on plasma cholesterol concentrations in hypercholesterolemic men. Am J Clin Nutr 53, 12051209.
Marquart, L, Slavin, JL & Fulcher, RG (2002) Whole-grain Foods in Health and Disease St Paul, MN American Association of Cereal Chemists Inc.
Marsono, Y, Illman, RJ, Clarke, JM, Trimble, RP & Topping, DL (1993) Plasma lipids and large bowel volatile fatty acids in pigs fed on white rice, brown rice and rice bran. Br J Nutr 70, 503513.
Martinez-Puig, D, Perez, JF, Castillo, M, Andaluz, A, Anguita, M, Morales, J & Gasa, J (2003) Consumption of raw potato starch increases colon length and fecal excretion of purine bases in growing pigs. J Nutr 133, 134139.
Mitchell, BL, Lawson, MJ, Davies, M, Kerr-Grant, A, Roediger, WEW, Illman, RJ & Topping, DL (1985) Volatile fatty acids in the human intestine: studies in surgical patients. Nutr Res 5, 10891092.
Morell, MK, Kosar-Hashemi, B, Cmiel, M, Samuel, MS, Chandler, P, Rahman, S, Buleon, A, Batey, IL & Li, Z (2003) Barley sex6 mutants lack starch synthase IIa activity and contain a starch with novel properties. Plant J 34, 173185.
Morita, T, Kasaoka, S, Hase, K & Kiriyama, S (1999) Psyllium shifts the fermentation site of high-amylose cornstarch toward the distal colon and increases fecal butyrate concentration in rats. J Nutr 129, 20812087.
National Health and, Medical Research Council (1997) Australian Code of Practice for the Care and Use of Animals for Scientific Purposes Canberra, Australia National Health and Medical Research Council.
Newman, RK, Lewis, SE, Newman, CW, Boik, RJ & Ramage, RT (1989) Hypocholesterolemic effect of barley foods on healthy men. Nutr Rep Int 39, 749760.
Pachenari, A, Conway, PL & Playne, MJ (2001) Bifidus-blood agar – a differentiating medium for the isolation and enumeration of bifidobacteria from faecal samples. Biosci Microflora 20, 8588.
Prentice, N, Qureshi, AA, Burger, WC & Elson, CE (1982) Response of hepatic cholesterol, fatty acid synthesis and activities of related enzymes to rolled barley and oats in chickens. Nutr Rep Int 26, 597604.
Roe, M, Brown, J, Faulks, R & Livesey, G (1996) Is the rat a suitable model for humans for studies of cereal digestion?. Eur J Clin Nutr 50, 710712.
Spiller, GA (2001) Definitions of dietary fiber. In CRC Handbook of Dietary Fiber in Human Nutrition, pp. 910, [Spiller, GA, editors]. Boca Raton, FL: CRC Press.
Stephen, AM, Haddad, AC & Phillips, SF (1983) Passage of carbohydrate into the colon. Direct measurements in humans. Gastroenterology 85, 589595.
Topcu, O, Karaday, K, Kuzu, MA, Ulukent, S, Erkek, B & Alacayir, I (2002) Enteral and intraluminal short-chain fatty acids improves ischemic left colonic anastomotic healing in the rat. Int J Colorectal Dis 17, 171176.
Topping, DL & Clifton, PM (2001) Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiol Rev 81, 10311064.
Topping, DL, Gooden, JM, Brown, IL, Biebrick, DA, McGrath, L, Trimble, RP, Choct, M & Illman, RJ (1997) A high amylose (amylomaize) starch raises proximal large bowel starch and increases colon length in pigs. J Nutr 127, 615622.
Topping, DL, Illman, RJ, Clarke, JM, Trimble, RP, Jackson, KA & Marsono, Y (1993) Dietary fat and fiber alter large bowel and portal venous volatile fatty acids and plasma cholesterol but not biliary steroids in pigs. J Nutr 123, 133143.
Topping, DL, Morell, MK, King, RA, Li, Z, Bird, AR & Noakes, M (2003) Resistant starch and health – Himalaya 292, a novel barley cultivar to deliver benefits to consumers. Starch 55, 539545.
Truswell, AS (2002) Cereal grains and coronary heart disease. Eur J Clin Nutr 56, 114.
Velazquez, OC, Lederer, HM & Rombeau, JL (1996) Butyrate and the colonocyte. Implications for neoplasia. Dig Dis Sci 41, 727739.
Wolever, TM (2003) Carbohydrate and the regulation of blood glucose and metabolism. Nutr Rev 61, S40S48.
Wursch, P, Pi-Sunyer, FX (1997) The role of viscous soluble fiber in the metabolic control of diabetes. A review with special emphasis on cereals rich in beta-glucan. Diabetes Care 20, 17741780.
Zhang, JX, Bergman, F, Hallmans, G, Johansson, G, Lundin, E, Stenling, R, Theander, O & Westerlund, E (1990) The influence of barley fibre on bile composition, gallstone formation, serum cholesterol and intestinal morphology in hamsters. APMIS 98, 568574.
Zhang, JX, Lundin, E, Andersson, H, Bosaeus, I, Dahlgren, S, Hallmans, G, Stenling, R & Aman, P (1991) Brewer's spent grain, serum lipids and fecal sterol excretion in human subjects with ileostomies. J Nutr 121, 778784.

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