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Klason lignin is a nutritionally heterogeneous fraction unsuitable for the prediction of forage neutral-detergent fibre digestibility in ruminants

  • Peter J. Van Soest (a1), James B. Robertson (a1), Mary B. Hall (a2) and Michael C. Barry (a3)

Abstract

Although lignin has been negatively correlated with neutral-detergent fibre (NDF) digestibility (NDFD) in ruminants and used to predict potential extent of NDF digestion of forages, selection of an analysis, Klason lignin (KL) or acid-detergent lignin (ADL), to describe that the nutritionally relevant lignin has not been resolved. Dismissed as an artifact is the difference between KL and ADL (ΔL). A question is whether ΔL influences NDFD. We evaluated the relationships of ΔL, KL and ADL with NDFD in order to determine the nutritionally homogeneous or heterogeneous nature of KL. Data sets from two laboratories (DS1 and DS2) were used that included ADL, KL and in vitro NDFD at 48 h (NDFD48). DS1 contained seven C3 grasses, seventeen C4 maize forages and nineteen alfalfas, and DS2 had fifteen C3 grasses, eight C4 forages and six alfalfas. Mean ΔL was greater than ADL in C3 and C4 samples and less in alfalfas. Within forage type and laboratory, ΔL was not correlated with NDFD48 (r −0·34–0·49; all P > 0·17). ADL was more consistently correlated with NDFD48 (r −0·47–−0·95; P < 0·01–0·21) than with KL (r 0·03–−0·91; P < 0·01–0·94). ΔL as a proportion of KL was correlated with NDFD48 in C3 and C4 samples (r 0·44–0·76; P < 0·01–0·08). The differing behaviours of ΔL and ADL relative to NDFD48 indicate that KL is a nutritionally heterogeneous fraction, the behaviour of which may vary by forage type and ratios of ADL and ΔL present.

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Corresponding author

*Corresponding author: Dr Mary B. Hall, email marybeth.hall@usda.gov

References

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1.National Research Council (2001) Nutrient Requirements of Dairy Cattle, 7th rev. ed.Washington, DC: National Academies Press.
2.Klason, JP (1908) Aussprache (Discussion) Die Verfahren der Holzzellstoff-Fabrikation. In Hauptversammlung Verein der Zellstoff- und Papier-Chemiker, pp. 5253. 23 and 24 November. Berlin: Papierhaus.
3.Theander, O, Aman, P, Westerlund, E, et al. (1995) Total dietary fiber determined as neutral sugar residues, uronic acid residues, and Klason lignin (the Uppsala method): collaborative study. J AOAC Int 78, 10301044.
4.Sullivan, JT (1959) A rapid method for the determination of acid insoluble lignin in forages and its relation to digestibility. J Anim Sci 18, 12921298.
5.Van Soest, PJ (1963) Use of detergents in the analysis of fibrous feeds: II. A rapid method for the determination of fiber and lignin. J AOAC 46, 825829.
6.Van Soest, PJ (1973) Collaborative study of acid detergent fiber and lignin. J AOAC 56, 781784.
7.Van Soest, PJ (1965) Use of detergents in the analysis of fibrous feeds. III. Study of effects of heating and drying on yield of fiber and lignin in forages. J AOAC 48, 785790.
8.Lowry, JB, Kennedy, PM & Conlan, LL (2002) Lignin in the ‘cell contents’ fraction of tropical forages. J Sci Food Agric 82, 370374.
9.Tedeschi, LO & Fox, DG (2016) The Ruminant Nutrition System. Acton, MA: XanEdu Publishing, Inc.
10.Hatfield, RD, Jung, HG, Ralph, J, et al. (1994) A comparison of the insoluble residues produced by the Klason lignin and acid detergent lignin procedures. J Sci Food Agric 65, 5158.
11.McSweeney, CS, Dulieu, A, Katayama, Y, et al. (1994) Solubilization of lignin by the ruminal anaerobic fungus Neocallimastix patriciarum. Appl Environ Microbiol 60, 29852989.
12.Jung, HG, Varel, VH, Weimer, PJ, et al. (1999) Accuracy of Klason lignin and acid detergent lignin methods as assessed by bomb calorimetry. J Agric Food Chem 47, 20052008.
13.Lowry, JB, Conlan, LL, Schlink, AC, et al. (1994) Acid detergent dispersible lignin in tropical grasses. J Sci Food Agric 65, 4149.
14.Raffrenato, E (2011) Physical, chemical and kinetic factors associated with fiber digestibility in ruminants and models describing these relationships. PhD Thesis, Cornell University, Ithaca, NY.
15.Gaillard, BDE & Richards, GN (1975) Presence of soluble lignin – carbohydrate complexes in the bovine rumen. Carbohydrate Res 42, 135145.
16.Ritter, GJ, Seborg, RM & Mitchell, RL (1932) Factors affecting gravimetric determination of lignin by the 72 % H2SO4 method. Ind Eng Chem Anal Ed 4, 202204.
17.McDougall, GJ, Stewart, D & Morrison, IM (1996) Tyrosine residues enhance cross-linking of synthetic proteins into lignin-like dehydrogenation products. Phytochemistry 41, 4347.
18.Kato, H (1956) Studies on browning reactions between sugars and amino compounds. J Agric Chem Soc Japan 20, 273283.
19.Norman, AG & Jenkins, SH (1934) The determination of lignin. II Errors introduced by the presence of proteins. Biochem J 28, 21602168.
20.Van Soest, PJ (2015) The Detergent System for Analysis of Foods and Feeds. Ithaca, NY: Cornell University.
21.Jung, HG, Mertens, DR & Payne, AJ (1997) Correlation of acid detergent lignin and Klason lignin with digestibility of forage dry matter and neutral detergent fiber. J Dairy Sci 80, 16221628.
22.Bidlack, JE & Buxton, DR (1992) Content and deposition rates of cellulose, hemicellulose, and lignin during regrowth of forage grasses and legumes. Can J Plant Sci 72, 809818.
23.Smith, LW, Goering, HK & Gordon, CH (1972) Relationships of forage compositions with rates of cell wall digestion and indigestibility of cell walls. J Dairy Sci 55, 11401147.
24.Neilson, MJ & Richards, GN (1978) The fate of the soluble lignin-carbohydrate complex produced in the bovine rumen. J Sci Food Agric 29, 513519.
25.Jung, HG, Valdez, FR, Hatfield, RD, et al. (1992) Cell wall composition and degradability of forage stems following chemical and biological delignification. J Sci Food Agric 58, 347355.
26.Sluiter, A, Hames, B, Ruiz, R, et al. (2012) Determination of structural carbohydrates and lignin in biomass. Laboratory analytical procedure. Technical report, NREL/TP-510–42618. Golden, CO: National Renewable Energy Laboratory.
27.Grabber, JH, Ralph, J & Hatfield, RD (2002) Model studies of ferulate-coniferyl alcohol cross-product formation in primary maize walls: implications for lignification in grasses. J Agric Food Chem 50, 60086016.
28.Chi, C, Zhang, Z, Chang, H, et al. (2009) Determination of furfural and hydroxymethylfurfural formed from biomass under acidic conditions. J Wood Chem Technol 29, 265276.
29.Wildschut, J, Smit, AT, Reith, JH, et al. (2013) Ethanol-based organosolv fractionation of wheat straw for the production of lignin and enzymatically digestible cellulose. Bioresour Technol 135, 5866.
30.Moon, FE & Abou-Raya, AK (1952) The lignin fraction of animal feeding-stuffs. II – Investigation of lignin determination procedures and development of a method for ‘acid-insoluble lignin’. J Sci Food Agric 3, 407418.
31.Mongeau, R & Brassard, R (1986) A rapid method for the determination of soluble and insoluble dietary fiber: comparison with AOAC total dietary fiber procedure and Englyst’s method. J Food Sci 51, 13331336.
32.Van Soest, PJ (1967) Development of a comprehensive system of feed analyses and its application to forages. J Anim Sci 26, 119128.
33.Weisbjerg, MR, Hvelplund, T & Søegaard, K (2004) Prediction of digestibility of neutral detergent solubles using the Lucas principle. J Anim Feed Sci 13, Suppl. 1, 239242.

Keywords

Klason lignin is a nutritionally heterogeneous fraction unsuitable for the prediction of forage neutral-detergent fibre digestibility in ruminants

  • Peter J. Van Soest (a1), James B. Robertson (a1), Mary B. Hall (a2) and Michael C. Barry (a3)

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