Hostname: page-component-8448b6f56d-gtxcr Total loading time: 0 Render date: 2024-04-18T15:42:38.164Z Has data issue: false hasContentIssue false
  • English
  • Français

Initial studies on leucine metabolism in the rumen of sheep

Published online by Cambridge University Press:  09 March 2007

M. Marsden ,
C. I. Bruce ,
C. G. Bartram  and
P. J. Buttery
M. Marsden
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
C. I. Bruce
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
C. G. Bartram
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
P. J. Buttery
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. [3H]leucine infused directly into the rumen of sheep labelled microbial protein and, when compared with the specific activity of the rumen free-leucine pool, indicated that 50% of the bacterial protein leucine originated from the rumen free-leucine pool.

2. The lower limit for bacterial protein turnover in the rumen was 0.37/d when calculated as the difference between the specific rate of disappearances of labelled bacteria from the rumen and the liquid-phase dilution rate.

3. Intravenously infused leucine also labelled the rumen bacteria. The build-up of specific activity in the rumen bacteria was sigmoidal and did not resemble that of the salivary protein which suggested that the rumen epithelium was a major endogenous protein input to the rumen. Additionally, bacteria isolated from the rumen epithelium had high radioactivity indicating that they were ingesting the rumen epithelial cells.

Type
General Nutrition Papers
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 161 - 171
Copyright
Copyright © The Nutrition Society 1988

References

Atkin, G. E. & Ferdinand, W. (1970) Analytical Biochemistry 38, 313–329.CrossRefGoogle Scholar
Bruce, C. I., Marsden, M. & Buttery, P. J. (1985) Proceedings of the Nutrition Society 44, 143A.CrossRefGoogle Scholar
Buttery, P. J., Marsden, M. & Nolan, J. V. (1983). In IVth International Symposium on Protein Metabolism and Nutrition, Clermont Ferrand, France, Vol. 2, pp. 275–278 [Arnal, M., Pion, R. and Bonin, D., editors]. Paris: INRA.Google Scholar
Cheng, K. J., McGowan, R. P. & Costerton, J. W. (1979) American Journal of Clinical Nutrition 32, 139–148.CrossRefGoogle Scholar
Cottle, D. J., Nolan, J. V. & Leng, R. A. (1978) Proceedings of the Australian Society of Animal Production 12, 138.Google Scholar
Davis, S. R., Barry, T. N. & Hughson, G. A. (1981) British Journal of Nutrition 46, 409–419.CrossRefGoogle Scholar
Faichney, G. J. (1975). In Digestion and Metabolism in the Ruminant, pp. 277–291 [McDonald, I.W. and Warner, A. C. I., editors]. Annidale, NSW: University of New England Publishing Unit.Google Scholar
Goodlad, R. A. (1981) Quarterly Journal of Experimental Physiology 66, 487–499.CrossRefGoogle Scholar
Hespell, R. B. & Bryant, M. P. (1979) Journal of Animal Science 49, 1640–1659.CrossRefGoogle Scholar
Hogan, J. P. & Weston, R. H. (1967) Australian Journal of Agricultural Research 18, 803–814.CrossRefGoogle Scholar
Kennedy, P. M. & Milligan, L. P. (1980) Canadian Journal of Animal Science 60, 1029–1032.CrossRefGoogle Scholar
Leibholz, J. (1969) Journul of Animal Science 29, 628–633.CrossRefGoogle Scholar
Ling, J. R & Buttery, P. J. (1978) British Journal of Nutrition 39, 165–179.CrossRefGoogle Scholar
Maeng, W. J. & Baldwin, R. L. (1976 a) Journal of Dairy Science 59, 636–642.CrossRefGoogle Scholar
Maeng, W. J. & Baldwin, R. L. (1976 b) Journal of Dairy Science 59, 643–647.CrossRefGoogle Scholar
Maeng, W. J. & Baldwin, R. L. (1976 c) Journal of Dairy Science 59, 648–655.CrossRefGoogle Scholar
Maeng, W. J., Van Nevel, C. J., Baldwin, R. L. & Morris, J. G. (1976) Journal of Dairy Science 59, 68–79.CrossRefGoogle Scholar
Mathers, J. C. & Miller, E. L. (1980) British Journal of Nutrition 43, 503–514.CrossRefGoogle Scholar
Mead, R. J. & Jones, G. A. (1981) Applied and Environmental Microbiology 41, 1020–1028.CrossRefGoogle Scholar
Nolan, J. V. & Leng, R. A. (1972) British Journal of Nutrition 27, 177–194.CrossRefGoogle Scholar
Nolan, J. V. & MacRae, J. C. (1976). Proceedings of the Nutrition Society 35, llOA.Google Scholar
Nolan, J. V. & Stachiw, S. (1979) British Journal of Nutrition 42, 63–80.CrossRefGoogle Scholar
Nugent, J. H. A., Jones, W. T., Jordan, D. J. & Mangan, J. L. (1983) British Journal of Nutrition 50, 357–368.CrossRefGoogle Scholar
Ørskov, E. R. & McDonald, N. A. (1982) Proceedings of the Nutrition Society 41, 16A.Google Scholar
Ørksov, E. R. & MacLeod, N. A. (1983) Proceedings of the Nutrition Society 42, 61A.Google Scholar
Patterson, J., Brightling, P. & Titchen, D. A. (1982) Quarterly Journal of Experimental Physiology 67, 57–67.CrossRefGoogle Scholar
Russel, J. B., Sniffen, C. J. & Van Soest, P. J. (1983) Journal of Dairy Science 66, 763–775.CrossRefGoogle Scholar
Salter, D. N., Daneshvar, K. & Smith, R. H. (1979) British Journal of Nutrition 41, 197–207.CrossRefGoogle Scholar
Siddons, R. C., Nolan, J. V., Beever, D. B. & MacRae, J. C. (1985) British Journal of Nutrition 54, 175–187.CrossRefGoogle Scholar
Singh, U. B., Varma, A., Verma, D. N., Lal, M. & Ranjhan, S. K. (1973) Journal of Agricultural Science, Cambridge 81, 349–351.CrossRefGoogle Scholar
Singh, U. B., Verma, D. N., Varma, A. & Ranjhan, S. K. (1974) Journal of Agricultural Science, Cambridge 83, 13–17.CrossRefGoogle Scholar
Sinnett-Smith, P. A., Dumelow, N. W. & Buttery, P. J. (1983) British Journal of Nutrition 50, 225–234.CrossRefGoogle Scholar
Van Soest, P. J. (1963) Journal of the Association of Oficial Agricultural Chemists 46, 829–835.Google Scholar
Vernon, B. G. & Buttery, P. J. (1976) British Journal of Nutrition 36, 575–579.CrossRefGoogle Scholar