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The identification of a phytohaemagglutinin in raw navy beans (Phaseolus vulguris L.) toxic for Japanese quail (Coturnix coturnix japonica)

Published online by Cambridge University Press:  09 March 2007

A. T. Andrews
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
D. J. Jayne-Williams
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
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Abstract

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1. Simple extracts of raw navy (haricot) beans (Phaseolus vulgaris L.) known to be toxic to conventional Japanese quail (Coturnix coturnix japonica) have been fractionated and tested for toxicity by feeding to this species.

2. Of the various fractions obtained during the biochemical purification of the phytohaemagglutinin, only those possessing agglutinating activity were found to be toxic.

3. The final purification stage yielded an homogeneous protein with both agglutinating and toxic properties.

Type
General Nutrition
Copyright
Copyright © The Nutrition Society 1974

References

REFERENCES

Andrews, A. T. (1974). Biochem. J. (In the Press.)Google Scholar
Evans, R. J., Pusztai, A., Watt, W. B. & Bauer, D. H. (1973). Biochim. biophys. Acta 303, 175.CrossRefGoogle Scholar
Hewitt, D. & Coates, M. E. (1969). Proc. Nutr. Soc. 28, 47A.Google Scholar
Hewitt, D., Coates, M. E., Kakade, M. L. & Liener, I. E. (1970). Proc. Nutr. Soc. 29, 15A.Google Scholar
Hewitt, D., Coates, M. E., Kakade, M. L. & Liener, I. E. (1973). Br. J. Nutr. 29, 423.CrossRefGoogle Scholar
Honavar, P. M., Shih, C.-V. & Liener, I. E. (1962). J. Nutr. 77, 109.CrossRefGoogle Scholar
Jaffé, W. G. & Hannig, K. (1965). Archs Biochem. Biophys. 109, 80.CrossRefGoogle Scholar
Jaffé, W. G. & Lette, C. L. V. (1968). J. Nutr. 94, 203.CrossRefGoogle Scholar
Jayne-Williams, D. J. (1973). Nature New Biol. 243, 150.CrossRefGoogle Scholar
Jayne-Williams, D. J. & Burgess, C. D. (1974). J. appl. Bact. (In the Press.)Google Scholar
Jayne-Williams, D. J. & Hewitt, D. (1972). J. appl. Bact. 35, 331.CrossRefGoogle Scholar
Kakade, M. L. & Evans, R. J. (1965 a). Br. J. Nutr. 19, 269.CrossRefGoogle Scholar
Kakade, M. L. & Evans, R. J. (1965 b). J. agric. Fd Chem. 13, 450.CrossRefGoogle Scholar
Kunitz, M. (1947). J. gen. Physiol. 30, 291.CrossRefGoogle Scholar
Liener, I. E. (1969). In Toxic Constituents of PZunt Foodstuffs p. 409 [Liener, I. E., editor’. New York and London: Academic Press.CrossRefGoogle Scholar
Rigas, D. A. & Johnson, E. A. (1964). Ann. N.Y. Acad. Sci. 113, 800.CrossRefGoogle Scholar
Stead, R. H., de Muelenaere, H. J. H. & Quicke, G. V. (1966). Archs Biochem. Biophys. 113, 703.CrossRefGoogle Scholar
Takahashi, T., Ramachandramurthy, P. & Liener, I. E. (1967). Biochim. biophys. Acta 133, 123.CrossRefGoogle Scholar