Skip to main content Accessibility help
×
Home

Fermented green tea extract exhibits hypolipidaemic effects through the inhibition of pancreatic lipase and promotion of energy expenditure

  • Dae-Bang Seo (a1), Hyun Woo Jeong (a2), Yeon-Ji Kim (a1), Sukyung Kim (a2), Jeongkee Kim (a2), Ji Hae Lee (a3), Kyungmi Joo (a4), Jin Kyu Choi (a5), Song Seok Shin (a2) and Sung-Joon Lee (a1)...

Abstract

Hyperlipidaemia is a major cause of atherosclerosis and related CVD and can be prevented with natural substances. Previously, we reported that a novel Bacillus-fermented green tea (FGT) exerts anti-obesity and hypolipidaemic effects. This study further investigated the hypotriglyceridaemic and anti-obesogenic effects of FGT and its underlying mechanisms. FGT effectively inhibited pancreatic lipase activity in vitro (IC50, 0·48 mg/ml) and ameliorated postprandial lipaemia in rats (26 % reduction with 500 mg/kg FGT). In hypertriglyceridaemic hamsters, FGT administration significantly reduced plasma TAG levels. In mice, FGT administration (500 mg/kg) for 2 weeks augmented energy expenditure by 22 % through the induction of plasma serotonin, a neurotransmitter that modulates energy expenditure and mRNA expressions of lipid metabolism genes in peripheral tissues. Analysis of the gut microbiota showed that FGT reduced the proportion of the phylum Firmicutes in hamsters, which could further contribute to its anti-obesity effects. Collectively, these data demonstrate that FGT decreases plasma TAG levels via multiple mechanisms including inhibition of pancreatic lipase, augmentation of energy expenditure, induction of serotonin secretion and alteration of gut microbiota. These results suggest that FGT may be a useful natural agent for preventing hypertriglyceridaemia and obesity.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Fermented green tea extract exhibits hypolipidaemic effects through the inhibition of pancreatic lipase and promotion of energy expenditure
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Fermented green tea extract exhibits hypolipidaemic effects through the inhibition of pancreatic lipase and promotion of energy expenditure
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Fermented green tea extract exhibits hypolipidaemic effects through the inhibition of pancreatic lipase and promotion of energy expenditure
      Available formats
      ×

Copyright

Corresponding author

* Corresponding authors: S.-J. Lee, fax +82 2 3290 3653, email junelee@korea.ac.kr; S. S. Shin, fax +82 31 281 8392, email ssshin@amorepacific.com

Footnotes

Hide All

These authors contributed equally.

Footnotes

References

Hide All
1. Kopelman, PG (2000) Obesity as a medical problem. Nature 404, 635643.
2. Williams, KJ (2008) Molecular processes that handle – and mishandle – dietary lipids. J Clin Invest 118, 32473259.
3. Cheung, O & Sanyal, AJ (2008) Abnormalities of lipid metabolism in nonalcoholic fatty liver disease. Semin Liver Dis 28, 351359.
4. Goodpaster, BH & Kelley, DE (2002) Skeletal muscle triglyceride: marker or mediator of obesity-induced insulin resistance in type 2 diabetes mellitus? Curr Diab Rep 2, 216222.
5. Raal, FJ (2009) Pathogenesis and management of the dyslipidemia of the metabolic syndrome. Metab Syndr Relat Disord 7, 8388.
6. Roden, M, Price, TB, Perseghin, G, et al. (1996) Mechanism of free fatty acid-induced insulin resistance in humans. J Clin Invest 97, 28592865.
7. Zraika, S, Dunlop, M, Proietto, J, et al. (2002) Effects of free fatty acids on insulin secretion in obesity. Obes Rev 3, 103112.
8. Nelson, RH (2013) Hyperlipidemia as a risk factor for cardiovascular disease. Prim Care 40, 195211.
9. Athyros, VG & Wierzbicki, AS (2013) Statin-fibrate combination therapy is safe and effective in normalizing lipid profile and in keeping cardiovascular event rates low. Curr Med Res Opin 30, 5758.
10. Fazio, S (2008) Management of mixed dyslipidemia in patients with or at risk for cardiovascular disease: a role for combination fibrate therapy. Clin Ther 30, 294306.
11. Fedele, D, Tiengo, A, Nosadini, R, et al. (1976) Hypolipidemic effects of metformin in hyperprebetalipoproteinemia. Diabete Metab 2, 127133.
12. Jensen, GB, Hilden, J, Als-Nielsen, B, et al. (2010) Statin treatment prevents increased cardiovascular and all-cause mortality associated with clarithromycin in patients with stable coronary heart disease. J Cardiovasc Pharmacol 55, 123128.
13. Brusq, JM, Ancellin, N, Grondin, P, et al. (2006) Inhibition of lipid synthesis through activation of AMP kinase: an additional mechanism for the hypolipidemic effects of berberine. J Lipid Res 47, 12811288.
14. Kim, WS, Lee, YS, Cha, SH, et al. (2009) Berberine improves lipid dysregulation in obesity by controlling central and peripheral AMPK activity. Am J Physiol Endocrinol Metab 296, E812E819.
15. Lee, YS, Kim, WS, Kim, KH, et al. (2006) Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states. Diabetes 55, 22562264.
16. Alshatwi, AA, Al Obaaid, MA, Al Sedairy, SA, et al. (2010) Black and green tea improves lipid profile and lipid peroxidation parameters in Wistar rats fed a high-cholesterol diet. J Physiol Biochem 67, 95104.
17. Amanolahi, F & Rakhshande, H (2013) Effects of ethanolic extract of green tea on decreasing the level of lipid profile in rat. Avicenna J Phytomed 3, 98105.
18. Cao, ZH, Gu, DH, Lin, QY, et al. (2010) Effect of pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity. Phytother Res 25, 234238.
19. Haidari, F, Shahi, MM, Zarei, M, et al. (2012) Effect of green tea extract on body weight, serum glucose and lipid profile in streptozotocin-induced diabetic rats. A dose response study. Saudi Med J 33, 128133.
20. Li, RW, Douglas, TD, Maiyoh, GK, et al. (2006) Green tea leaf extract improves lipid and glucose homeostasis in a fructose-fed insulin-resistant hamster model. J Ethnopharmacol 104, 2431.
21. Serisier, S, Leray, V, Poudroux, W, et al. (2008) Effects of green tea on insulin sensitivity, lipid profile and expression of PPARalpha and PPARgamma and their target genes in obese dogs. Br J Nutr 99, 12081216.
22. Skrzydlewska, E, Ostrowska, J, Farbiszewski, R, et al. (2002) Protective effect of green tea against lipid peroxidation in the rat liver, blood serum and the brain. Phytomedicine 9, 232238.
23. Toyoda-Ono, Y, Yoshimura, M, Nakai, M, et al. (2007) Suppression of postprandial hypertriglyceridemia in rats and mice by oolong tea polymerized polyphenols. Biosci Biotechnol Biochem 71, 971976.
24. Vinson, JA & Dabbagh, YA (1998) Effect of green and black tea supplementation on lipids, lipid oxidation and fibrinogen in the hamster: mechanisms for the epidemiological benefits of tea drinking. FEBS Lett 433, 4446.
25. Yang, M, Wang, C & Chen, H (2001) Green, oolong and black tea extracts modulate lipid metabolism in hyperlipidemia rats fed high-sucrose diet. J Nutr Biochem 12, 1420.
26. Lee, SJ & Jia, Y (2015) The effect of bioactive compounds in tea on lipid metabolism and obesity through regulation of peroxisome proliferator-activated receptors. Curr Opin Lipidol 26, 39.
27. Walkowiak, J, Bajerska, J, Kargulewicz, A, et al. (2013) Single dose of green tea extract decreases lipid digestion and absorption from a test meal in humans. Acta Biochim Pol 60, 481483.
28. Basu, A, Sanchez, K, Leyva, MJ, et al. (2010) Green tea supplementation affects body weight, lipids, and lipid peroxidation in obese subjects with metabolic syndrome. J Am Coll Nutr 29, 3140.
29. Erba, D, Riso, P, Bordoni, A, et al. (2005) Effectiveness of moderate green tea consumption on antioxidative status and plasma lipid profile in humans. J Nutr Biochem 16, 144149.
30. Davies, MJ, Judd, JT, Baer, DJ, et al. (2003) Black tea consumption reduces total and LDL cholesterol in mildly hypercholesterolemic adults. J Nutr 133, 3298S3302S.
31. Fujita, H & Yamagami, T (2008) Antihypercholesterolemic effect of Chinese black tea extract in human subjects with borderline hypercholesterolemia. Nutr Res 28, 450456.
32. Zhao, Y, Asimi, S, Wu, K, et al. (2015) Black tea consumption and serum cholesterol concentration: systematic review and meta-analysis of randomized controlled trials. Clin Nutr 34, 612619.
33. Singh, DK, Banerjee, S & Porter, TD (2009) Green and black tea extracts inhibit HMG-CoA reductase and activate AMP kinase to decrease cholesterol synthesis in hepatoma cells. J Nutr Biochem 20, 816822.
34. Hsu, TF, Kusumoto, A, Abe, K, et al. (2006) Polyphenol-enriched oolong tea increases fecal lipid excretion. Eur J Clin Nutr 60, 13301336.
35. Seo, DB, Jeong, HW, Cho, D, et al. (2015) Fermented green tea extract alleviates obesity and related complications and alters gut microbiota composition in diet-induced obese mice. J Med Food 18, 549556.
36. Nakai, M, Fukui, Y, Asami, S, et al. (2005) Inhibitory effects of oolong tea polyphenols on pancreatic lipase in vitro . J Agric Food Chem 53, 45934598.
37. Chun, J, Kim, KY, Lee, JH, et al. (2010) The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer. BMC Microbiol 10, 101.
38. Kim, OS, Cho, YJ, Lee, K, et al. (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62, 716721.
39. Hoang, MH, Jia, Y, Jun, HJ, et al. (2012) Taurine is a liver X receptor-alpha ligand and activates transcription of key genes in the reverse cholesterol transport without inducing hepatic lipogenesis. Mol Nutr Food Res 56, 900911.
40. Watanabe, H, Rose, MT & Aso, H (2011) Role of peripheral serotonin in glucose and lipid metabolism. Curr Opin Lipidol 22, 186191.
41. Watanabe, H, Akasaka, D, Ogasawara, H, et al. (2010) Peripheral serotonin enhances lipid metabolism by accelerating bile acid turnover. Endocrinology 151, 47764786.
42. Ley, RE, Backhed, F, Turnbaugh, P, et al. (2005) Obesity alters gut microbial ecology. Proc Natl Acad Sci U S A 102, 1107011075.
43. DiBaise, JK, Zhang, H, Crowell, MD, et al. (2008) Gut microbiota and its possible relationship with obesity. Mayo Clin Pro 83, 460469.
44. Qiao, Y, Sun, J, Xia, S, et al. (2014) Effects of resveratrol on gut microbiota and fat storage in a mouse model with high-fat-induced obesity. Food Funct 5, 12411249.
45. Vrablik, M & Ceska, R (2015) Treatment of hypertriglyceridemia: a review of current options. Physiol Res 64, Suppl. 3, S331S340.
46. Digby, JE, Ruparelia, N & Choudhury, RP (2012) Niacin in cardiovascular disease: recent preclinical and clinical developments. Arterioscler Thromb Vasc Biol 32, 582588.
47. Briand, O, Touche, V, Colin, S, et al. (2016) Liver X receptor regulates triglyceride absorption through intestinal down-regulation of scavenger receptor class B, type 1. Gastroenterology 150, 650658.
48. Zhang, K, Li, L, Qi, Y, et al. (2012) Hepatic suppression of Foxo1 and Foxo3 causes hypoglycemia and hyperlipidemia in mice. Endocrinology 153, 631646.
49. Geldenhuys, WJ, Lin, L, Darvesh, AS, et al. (2016) Emerging strategies of targeting lipoprotein lipase for metabolic and cardiovascular diseases. Drug Discov Today. (epublication ahead of print version 19 October 2016).
50. Naik, R, Obiang-Obounou, BW, Kim, M, et al. (2014) Therapeutic strategies for metabolic diseases: small-molecule diacylglycerol acyltransferase (DGAT) inhibitors. Chem Med Chem 9, 24102424.
51. Lunagariya, NA, Patel, NK, Jagtap, SC, et al. (2014) Inhibitors of pancreatic lipase: state of the art and clinical perspectives. EXCLI J 13, 897921.
52. Torgerson, JS, Hauptman, J, Boldrin, MN, et al. (2004) XENical in the prevention of diabetes in obese subjects (XENDOS) study: a randomized study of orlistat as an adjunct to lifestyle changes for the prevention of type 2 diabetes in obese patients. Diabetes Care 27, 155161.
53. Young, SN (2007) How to increase serotonin in the human brain without drugs. J Psychiatry Neurosci 32, 394399.
54. Finck, BN & Kelly, DP (2006) PGC-1 coactivators: inducible regulators of energy metabolism in health and disease. J Clin Invest 116, 615622.
55. Hardie, DG (2007) AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy. Nat Rev Mol Cell Biol 8, 774785.
56. Li, X (2013) SIRT1 and energy metabolism. Acta Biochim Biophys Sin (Shanghai) 45, 5160.
57. Lee, JE, Lee, BJ, Chung, JO, et al. (2011) 1H NMR-based metabolomic characterization during green tea (Camellia sinensis) fermentation. Food Res Int 44, 597604.
58. Doan, KV, Ko, CM, Kinyua, AW, et al. (2015) Gallic acid regulates body weight and glucose homeostasis through AMPK activation. Endocrinology 156, 157168.
59. Cai, EP & Lin, JK (2009) Epigallocatechin gallate (EGCG) and rutin suppress the glucotoxicity through activating IRS2 and AMPK signaling in rat pancreatic beta cells. J Agric Food Chem 57, 98179827.
60. Liu, HW, Chan, YC, Wang, MF, et al. (2015) Dietary (-)-epigallocatechin-3-gallate supplementation counteracts aging-associated skeletal muscle insulin resistance and fatty liver in senescence-accelerated mouse. J Agric Food Chem 63, 84078417.
61. Xiao, N, Mei, F, Sun, Y, et al. (2014) Quercetin, luteolin, and epigallocatechin gallate promote glucose disposal in adipocytes with regulation of AMP-activated kinase and/or sirtuin 1 activity. Planta Med 80, 9931000.
62. Vermeer, MA, Mulder, TP & Molhuizen, HO (2008) Theaflavins from black tea, especially theaflavin-3-gallate, reduce the incorporation of cholesterol into mixed micelles. J Agric Food Chem 56, 1203112036.
63. Qin, J, Li, Y, Cai, Z, et al. (2012) A metagenome-wide association study of gut microbiota in type 2 diabetes. Nature 490, 5560.
64. Sanz, Y, Santacruz, A & Gauffin, P (2010) Gut microbiota in obesity and metabolic disorders. Proc Nutr Soc 69, 434441.
65. Ley, RE, Turnbaugh, PJ, Klein, S, et al. (2006) Microbial ecology: human gut microbes associated with obesity. Nature 444, 10221023.
66. Petriz, BA, Castro, AP, Almeida, JA, et al. (2014) Exercise induction of gut microbiota modifications in obese, non-obese and hypertensive rats. BMC Genomics 15, 511.
67. An, C, Kuda, T, Yazaki, T, et al. (2013) FLX pyrosequencing analysis of the effects of the brown-algal fermentable polysaccharides alginate and laminaran on rat cecal microbiotas. Appl Environ Microbiol 79, 860866.
68. Zhang, X, Zhao, Y, Zhang, M, et al. (2012) Structural changes of gut microbiota during berberine-mediated prevention of obesity and insulin resistance in high-fat diet-fed rats. PLOS ONE 7, e42529.
69. Kasai, C, Sugimoto, K, Moritani, I, et al. (2015) Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing. BMC Gastroenterol 15, 100.

Keywords

Type Description Title
UNKNOWN
Supplementary materials

Seo supplementary material
Figures S1-S3

 Unknown (79 KB)
79 KB
WORD
Supplementary materials

Seo supplementary material
Tables S1-S3

 Word (16 KB)
16 KB

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed