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Enzymatically modified isoquercitrin improves endothelial function in volunteers at risk of cardiovascular disease

  • Nicola P. Bondonno (a1) (a2), Catherine P. Bondonno (a1) (a2), Natalie C. Ward (a3) (a4), Richard J. Woodman (a5), Jonathan M. Hodgson (a1) (a2) and Kevin D. Croft (a1)...

Abstract

A higher intake of food rich in flavonoids such as quercetin can reduce the risk of CVD. Enzymatically modified isoquercitrin (EMIQ®) has a bioavailability 17-fold higher than quercetin aglycone and has shown potential CVD moderating effects in animal studies. The present study aimed to determine whether acute ingestion of EMIQ® improves endothelial function, blood pressure (BP) and cognitive function in human volunteers at risk of CVD. Twenty-five participants (twelve males and thirteen females) with at least one CVD risk factor completed this randomised, controlled, crossover study. In a random order, participants were given EMIQ® (2 mg aglycone equivalent)/kg body weight or placebo alongside a standard breakfast meal. Endothelial function, assessed by flow-mediated dilatation (FMD) of the brachial artery was measured before and 1·5 h after intervention. BP, arterial stiffness, cognitive function, BP during cognitive stress and measures of quercetin metabolites, oxidative stress and markers of nitric oxide (NO) production were assessed post-intervention. After adjustment for pre-treatment measurements and treatment order, EMIQ® treatment resulted in a significantly higher FMD response compared with the placebo (1·80 (95 % CI 0·23, 3·37) %; P = 0·025). Plasma concentrations of quercetin metabolites were significantly higher (P < 0·001) after EMIQ® treatment compared with the placebo. No changes in BP, arterial stiffness, cognitive function or biochemical parameters were observed. In this human intervention study, the acute administration of EMIQ® significantly increased circulating quercetin metabolites and improved endothelial function. Further clinical trials are required to assess whether health benefits are associated with long-term EMIQ® consumption.

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Corresponding author

*Corresponding author: N. P. Bondonno, email n.bondonno@ecu.edu.au

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Clinical trial registry number and website: Australian New Zealand Clinical Trial Registry (ACTRN12617001202358); http://www.anzctr.org.au/

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References

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Keywords

Enzymatically modified isoquercitrin improves endothelial function in volunteers at risk of cardiovascular disease

  • Nicola P. Bondonno (a1) (a2), Catherine P. Bondonno (a1) (a2), Natalie C. Ward (a3) (a4), Richard J. Woodman (a5), Jonathan M. Hodgson (a1) (a2) and Kevin D. Croft (a1)...

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