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Effects of methionine supplementation on the expression of oxidative stress-related genes in acute heat stress-exposed broilers

  • Ana Paula Del Vesco (a1), Eliane Gasparino (a1), Daiane de Oliveira Grieser (a1), Vittor Zancanela (a1), Maria Amélia Menck Soares (a2) and Adhemar Rodrigues de Oliveira Neto (a3)...

Abstract

The aim of the present study was to evaluate the effects of heat stress (HS) and methionine supplementation on the markers of stress and on the gene expression levels of uncoupling proteins (UCP), betaine–homocysteine methyltransferase (BHMT), cystathionine β-synthase (CBS), glutathione synthetase (GSS) and glutathione peroxidase 7 (GPx7). Broilers from 1 to 21 d and from 22 to 42 d of age were divided into three treatment groups related to methionine supplementation: without methionine supplementation (MD); recommended level of methionine supplementation (DL1); excess methionine supplementation (DL2). The broilers were either kept at a comfortable thermal temperature or exposed to HS (38°C for 24 h). During the starter period, we observed the effects of the interaction between diet and environment on the gene expression levels of UCP, BHMT and GSS. Higher gene expression levels of UCP and BHMT were observed in broilers that were maintained at thermal comfort conditions and received the MD diet. HS broilers fed the DL1 and DL2 diets had the highest expression level of GSS. The expression levels of the CBS and GPx7 genes were influenced by both the environment and methionine supplementation. During the grower period, the gene expression levels of BHMT, CBS, GSS and GPx7 were affected by the diet × environment interaction. A higher expression level of BHMT was observed in broilers maintained at thermal comfort conditions and on the MD diet. HS induced higher expression levels of CBS, GSS and GPx7 in broilers that received the DL1 and DL2 diets. The present results suggest that under HS conditions, methionine supplementation could mitigate the effects of stress, since methionine contributed to the increased expression levels of genes related to antioxidant activity.

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Corresponding author

* Corresponding author: E. Gasparino, fax +55 44 3011 4729, email egasparino@uem.br

References

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1 Moustafa, MH, Sharma, RK, Thornton, J, et al. (2004) Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Human Reprod 19, 129138.
2 Lee, HC & Wei, YH (2005) Mitochondrial biogenesis and mitochondrial DNA maintenance of mammalian cells under oxidative stress. Int J Biochem Cell Biol 37, 822834.
3 Lee, J, Giordano, S & Zhang, J (2012) Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling. Biochem J 441, 523540.
4 Aiken, CT, Kaake, RM, Wang, X, et al. (2011) Oxidative stress-mediated regulation of proteasome complexes. Mol Cell Proteomics 10, R110.006924.
5 Gu, X, Sun, J, Li, S, et al. (2013) Oxidative stress induces DNA demethylation and histone acetylation in SH-SY5Y cells: potential epigenetic mechanisms in gene transcription in Aβ production. Neurobiol Aging 34, 10691079.
6 Mujahid, A, Sato, K, Akiba, Y, et al. (2006) Acute heat stress stimulates mitochondrial superoxide production in broiler skeletal muscle, possibly via downregulation of uncoupling protein content. Poult Sci 85, 12591265.
7 Mujahid, A, Akiba, Y & Toyomizu, M (2009) Olive oil-supplemented diet alleviates acute heat stress-induced mitochondrial ROS production in chicken skeletal muscle. Am J Physiol Regul Integr Comp Physiol 297, R690R698.
8 Yang, L, Tan, GY, Fu, QF, et al. (2010) Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration, ROS production and lipid peroxidation in broiler chickens. Comp Biochem Physiol C Toxicol Pharmacol 151, 204208.
9 Geraert, PA, Padilha, JCF & Guillaumin, S (1996) Metabolic and endocrine changes induced by chronic heat exposure chickens: biological and endocrinological variables. Br J Nutr 75, 205216.
10 Yunianto, V, Hayashi, K, Kaneda, S, et al. (1997) Effect of environmental temperature on muscle protein turnover and heat production in tube-fed broiler chicken. Br J Nutr 77, 897909.
11 Willemsen, H, Swennen, Q, Everaert, N, et al. (2011) Effects of dietary supplementation of methionine and its hydroxy analog dl-2-hydroxy-4-methylthiobutanoic acid on growth performance, plasma hormone levels, and the redox status of broiler chickens expose to high temperatures. Poult Sci 90, 23112320.
12 Del Vesco, AP, Gasparino, E, Grieser, DO, et al. (2014) Effects of methionine supplementation on the redox state of acute heat stress-exposed quails. J Anim Sci 92, 806815.
13 Ronsein, GE, Miyamoto, S, Bechara, E, et al. (2006) Oxidação de proteínas por oxigênio singlete: mecanismos de dano, estratégias para detecção e implicações biológicas (Protein oxidation by singlet oxygen: damage mechanisms, strategies for detection and biological implications). Química Nova 29, 563568.
14 Gasparino, E, Voltolini, DM, Del Vesco, AP, et al. (2013) IGF-I, GHR and UCP mRNA expression in the liver and muscle of high- and low-feed-efficiency laying Japanese quail at different environmental temperatures. Livest Sci 157, 339344.
15 Ledesma, A, Lacoba, MG & Rial, E (2000) The mitochondrial uncoupling proteins. Genome Biol 3, 3015.13015.9.
16 Abe, T, Mujahid, A, Sato, K, et al. (2006) Possible role of avian uncoupling protein in down-regulating mitochondrial superoxide production in skeletal muscle of fasted chickens. FEBS Lett 580, 48154822.
17 Halliwell, B & Gutteridge, JMC (2001) Free Radicals in Biology and Medicine, 3rd ed. New York: Oxford University Press.
18 Huber, PC, Almeida, WP & Fátima, A (2008) Glutationa e enzimas relacionadas: Papel biológico e importância em processos patológicos (Glutathione and related enzymes: biological role and importance in pathological processes). Química Nova 31, 11701179.
19 Shoveller, AK, Stoll, B, Ball, RO, et al. (2005) Nutritional and functional importance of intestinal sulfur amino acid metabolism. J Nutr 135, 16091612.
20 Mosharov, E, Cranford, MR & Banerjee, R (2000) The quantitatively important relationship between homocysteine metabolism and glutathione synthesis by the transsulfuration pathway and its regulation by redox changes. Biochemistry 39, 1300513011.
21 Persa, C, Pierce, A, Ma, Z, et al. (2004) The presence of a transsulfuration pathway in the lens: a new oxidative stress defense system. Exp Eye Res 79, 875886.
22 Rostagno, HS, Albino, LFT, Donzele, JL, et al. (2011) Brazilian Tables for Birds and Pigs: Composition of Foods and Nutritional Requirements, 3rd ed. Viçosa: Universidade Federal de Viçosa.
23 Mujahid, A, Yoshiki, Y, Akiba, Y, et al. (2005) Super-oxide radical production in chicken skeletal muscle induced by acute heat stress. Poult Sci 84, 307314.
24 Lin, H, Decuypere, E & Buyse, J (2006) Acute heat stress induces oxidative stress in broiler chickens. Comp Biochem Physiol Mol Integr Physiol 144, 1117.
25 Tan, GY, Yang, L, Fu, QF, et al. (2010) Effects of different acute high ambient temperatures on function of hepatic mitochondrial respiration, antioxidative enzymes, and oxidative injury in broiler chickens. Poult Sci 89, 115122.
26 Bai, Z, Harvey, LM & McNeil, B (2003) Elevated temperature effects on the oxidant/antioxidant balance in submerged batch cultures of the filamentous fungus Aspergillus niger B1. Biotechnol Bioeng 83, 772779.
27 Fink, BD, Rezka, KJ, Herlein, JA, et al. (2005) Respiratory uncoupling by UCP1 and UCP2 and superoxide generation in endothelial cell mitochondria. Am J Physiol Endocrinol Metab 288, E71E79.
28 Evock-Clover, CM, Poch, SM, Richards, MP, et al. (2002) Expression of an uncoupling protein gene homologue in chickens. Comp Biochem Physiol A Mol Integr Physiol 133, 345358.
29 Del Vesco, AP, Gasparino, E, Oliveira Neto, AR, et al. (2013) Effect of methionine supplementation on mitochondrial genes expression in the breast muscle and liver of broilers. Livest Sci 151, 284291.
30 Ojano-Dirain, C, Toyomizu, M, Wing, T, et al. (2007) Gene expression in breast muscle and duodenum from low and high feed efficient broilers. Poult Sci 86, 372381.
31 Raimbault, S, Dridi, S, Denjean, F, et al. (2001) An uncoupling protein homologue putatively involved in facultative thermogenesis in birds. Biochem J 353, 441444.
32 Kuss, F (2005) Agentes oxidantes e antioxidants (Oxidizing agents and antioxidants). In Seminário apresentado na disciplina bioquímica do tecido animal, no Programa de Pós-Graduação em Ciências Veterinárias da Universidade Federal do Rio Grande do Sul em 2005 (Seminar Presented at the Discipline of Animal Tissue Biochemistry in the Graduate Program in Veterinary Sciences, Federal University of Rio Grande do Sul, 2005). Porto Alegre: Federal University of Rio Grande do Sul. http://www6.ufrgs.br/favet/lacvet/restrito/pdf/ag_oxid_antioxid.pdf.
33 Morand, C, Rios, L, Moundras, C, et al. (1997) Influence of methionine availability on glutathione synthesis and delivery by the liver. J Nutr Biochem 8, 246255.
34 Stipanuk, MH (2004) Sulfur amino acid metabolism: pathways for production and removal of homocysteine and cysteine. Annu Rev Nutr 24, 539577.
35 Finkelstein, JD (1998) The metabolism of homocysteine: pathways and regulation. Eur J Pediatr 157, S40S44.
36 Dietrich-Muszalska, A, Malinowska, J, Olas, B, et al. (2012) The oxidative stress may be induced by the elevated homocysteine in schizophrenic patients. Neurochem Res 37, 10571062.
37 Ramakrishnan, S, Sulochana, KN, Lakshmi, S, et al. (2006) Biochemistry of homocysteine in health and diseases. Indian J Biochem Biophys 43, 275283.
38 Sautin, YY & Johnson, RJ (2008) Uric acid: the oxidant–antioxidant paradox. Nucleosides Nucleotides Nucleic Acids 27, 608619.
39 Klandorf, H, Rathore, D, Iqbal, M, et al. (2001) Accelerated tissue aging and increased oxidative stress in broiler chickens fed allopurinol. Comp Biochem Physiol C Toxicol Pharmacol 129, 93104.
40 Simoyi, M, Van Dyke, K & Klandorf, H (2002) Manipulation of plasma uric acid broiler chicks and its effect on leukocyte oxidative activity. Am J Physiol Regul Integr Comp Physiol 282, R791R796.
41 Bunchasak, C, Sooksridang, T & Chaiyapit, R (2006) Effect of adding methionine hydroxy analogue as methionine source at the commercial requirement recommendation on production performance and evidence of ascites syndrome of male broiler chicks fed corn-soybean based. Int J Poult Sci 5, 744752.
42 Machin, M, Simoyi, MF, Blemings, KP, et al. (2004) Increased dietary protein elevates plasma uric acid and is associated with decreased oxidative stress in rapidly-growing broilers. Comp Biochem Physiol B Biochem Mol Biol 137, 383390.
43 Glaser, V, Leipnitz, G, Straliotto, MR, et al. (2010) Oxidative stress-mediated inhibition of brain creatine kinase activity by methylmercury. Neurotoxicology 31, 454460.
44 Aksenova, M, Butterfield, DA, Zhang, SX, et al. (2002) Increased protein oxidation and decreased creatine kinase BB expression and activity after spinal cord contusion injury. J Neurotrauma 19, 491502.
45 Gunst, JJ, Langlois, MR, Delanghe, JR, et al. (1998) Serum creatine kinase activity is not a reliable marker for muscle damage in conditions associated with low extracellular glutathione concentration. Clin Chem 44, 939943.
46 Khan, HA, Alhomida, AS, Sobki, SH, et al. (2013) Serum markers of tissue damage and oxidative stress in patients with acute myocardial infarction. Biomed Res 24, 1520.
47 Kumar Das, S, Hiran, KR, Mukherjee, S, et al. (2007) Oxidative stress is the primary event: effects of ethanol consumption in brain. Indian J Clin Biochem 22, 99104.
48 Mokondjimobe, E, Longo-Mbenza, B, Akiana, J, et al. (2012) Biomarkers of oxidative stress and personalized treatment of pulmonary tuberculosis: emerging role of γ-glutamyltransferase. Adv Pharmacol Sci 2012, 465634.
49 Ismail, IB, Al-Busadah, KA & El-Bahr, SM (2013) Oxidative stress biomarkers and biochemical profile in broilers chicken fed zinc bacitracin and ascorbic acid under hot climate. Am J Biochem Mol Biol 3, 202214.

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Effects of methionine supplementation on the expression of oxidative stress-related genes in acute heat stress-exposed broilers

  • Ana Paula Del Vesco (a1), Eliane Gasparino (a1), Daiane de Oliveira Grieser (a1), Vittor Zancanela (a1), Maria Amélia Menck Soares (a2) and Adhemar Rodrigues de Oliveira Neto (a3)...

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