Skip to main content Accessibility help
×
Home

Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2

  • Shihai Zhang (a1), Qing Yang (a1), Man Ren (a1) (a2), Shiyan Qiao (a1), Pingli He (a1), Defa Li (a1) and Xiangfang Zeng (a1)...

Abstract

Knowledge of regulation of glucose transport contributes to our understanding of whole-body glucose homoeostasis and human metabolic diseases. Isoleucine has been reported to participate in regulation of glucose levels in many studies; therefore, this study was designed to examine the effect of isoleucine on intestinal and muscular GLUT expressions. In an animal experiment, muscular GLUT and intestinal GLUT were determined in weaning pigs fed control or isoleucine-supplemented diets. Supplementation of isoleucine in the diet significantly increased piglet average daily gain, enhanced GLUT1 expression in red muscle and GLUT4 expression in red muscle, white muscle and intermediate muscle (P<0·05). In additional, expressions of Na+/glucose co-transporter 1 and GLUT2 were up-regulated in the small intestine when pigs were fed isoleucine-supplemented diets (P<0·05). C2C12 cells were used to examine the expressions of muscular GLUT and glucose uptake in vitro. In C2C12 cells supplemented with isoleucine in the medium, cellular 2-deoxyglucose uptake was increased (P<0·05) through enhancement of the expressions of GLUT4 and GLUT1 (P<0·05). The effect of isoleucine was greater than that of leucine on glucose uptake (P<0·05). Compared with newborn piglets, 35-d-old piglets have comparatively higher GLUT4, GLUT2 and GLUT5 expressions. The results of this study demonstrated that isoleucine supplementation enhanced the intestinal and muscular GLUT expressions, which have important implications that suggest that isoleucine could potentially increase muscle growth and intestinal development by enhancing local glucose uptake in animals and human beings.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2
      Available formats
      ×

Copyright

Corresponding author

* Corresponding author: X. Zeng, fax +86 106 273 3688, email zengxf@cau.edu.cn

References

Hide All
1. Linda, C, Flynn, AN, Turner, JR, et al. (2005) SGLT-1-mediated glucose uptake protects intestinal epithelial cells against LPS-induced apoptosis and barrier defects: a novel cellular rescue mechanism? FASEB J 19, 18221835.
2. Ciaraldi, TP, Mudaliar, S, Barzin, A, et al. (2005) Skeletal muscle GLUT1 transporter protein expression and basal leg glucose uptake are reduced in type 2 diabetes. J Clin Endocr Metab 90, 352358.
3. Kim, JK, Zisman, A, Fillmore, JJ, et al. (2001) Glucose toxicity and the development of diabetes in mice with muscle-specific inactivation of GLUT4. J Clin Invest 108, 153.
4. Bryant, NJ, Govers, R & James, DE (2002) Regulated transport of the glucose transporter GLUT4. Nat Rev Mol Cell Bio 3, 267277.
5. Piper, RC, Hess, LJ & James, DE (1991) Differential sorting of two glucose transporters expressed in insulin-sensitive cells. Am J Physiol 260, C570C580.
6. Slot, JW, Geuze, HJ, Gigengack, S, et al. (1991) Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat. Proc Natl Acad Sci U S A 88, 78157819.
7. Rodnick, KJ, Slot, J, Studelska, D, et al. (1992) Immunocytochemical and biochemical studies of GLUT4 in rat skeletal muscle. J Biol Chem 267, 62786285.
8. Ferraris, RP & Diamond, J (1997) Regulation of intestinal sugar transport. Physiol Rev 77, 257302.
9. Kojima, T, Nishimura, M, Yajima, T, et al. (1999) Developmental changes in the regional Na+/glucose transporter mRNA along the small intestine of suckling rats. Comp Biochem Phys B 122, 8995.
10. Wright, EM, Hirayama, B & Loo, D (2007) Active sugar transport in health and disease. J Intern Med 261, 3243.
11. Burant, CF & Saxena, M (1994) Rapid reversible substrate regulation of fructose transporter expression in rat small intestine and kidney. Am J Physiol 267, G71G79.
12. Douard, V & Ferraris, RP (2008) Regulation of the fructose transporter GLUT5 in health and disease. Am J Physiol Endocrinol Metab 295, E227E237.
13. Shirazi-Beechey, SP (1995) Molecular biology of intestinal glucose transport. Nutr Res Rev 8, 2741.
14. Moran, AW, Al-Rammahi, MA, Arora, DK, et al. (2010) Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104, 637646.
15. Moran, AW, Al-Rammahi, MA, Arora, DK, et al. (2010) Expression of Na+/glucose co-transporter 1 (SGLT1) in the intestine of piglets weaned to different concentrations of dietary carbohydrate. Br J Nutr 104, 647655.
16. Ryan, KK & Seeley, RJ (2013) Food as a hormone. Science 339, 918.
17. Bernard, JR, Liao, YH, Doerner, PG, et al. (2012) An amino acid mixture is essential to optimize insulin-stimulated glucose uptake and GLUT4 translocation in perfused rodent hindlimb muscle. J Appl Physiol 113, 97104.
18. Doi, M, Yamaoka, I, Fukunaga, T, et al. (2003) Isoleucine, a potent plasma glucose-lowering amino acid, stimulates glucose uptake in C2C12 myotubes. Biochem Bioph Res Co 312, 11111117.
19. Nishitani, S, Takehana, K, Fujitani, S, et al. (2005) Branched-chain amino acids improve glucose metabolism in rats with liver cirrhosis. Am J Physiol Gastr L 288, G1292G1300.
20. Doi, M, Yamaoka, I, Nakayama, M, et al. (2005) Isoleucine, a blood glucose-lowering amino acid, increases glucose uptake in rat skeletal muscle in the absence of increases in AMP-activated protein kinase activity. J Nutr 135, 21032108.
21. National Research Council (1998) Nutrient Requirements of Swine, 10th rev. ed. Washington, DC: National Academies Press.
22. Association of Official Analytical Chemists (2003) Official Methods of Analysis. Arlington, VA: AOAC.
23. Opapeju, F, Krause, D, Payne, R, et al. (2009) Effect of dietary protein level on growth performance, indicators of enteric health, and gastrointestinal microbial ecology of weaned pigs induced with postweaning colibacillosis. J Anim Sci 87, 26352643.
24. Zhang, S, Qiao, S, Ren, M, et al. (2013) Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs. Amino acids 45, 11911205.
25. Stokes, JL, Gunness, M, Dwyer, IM, et al. (1945) Microbiological methods for the determination of amino acids II. A uniform assay for the ten essential amino acids. J Biol Chem 160, 3549.
26. Figueroa, J, Lewis, A, Miller, P, et al. (2003) Growth, carcass traits, and plasma amino acid concentrations of gilts fed low-protein diets supplemented with amino acids including histidine, isoleucine, and valine. J Anim Sci 81, 15291537.
27. Lordelo, M, Gaspar, A, Le Bellego, L, et al. (2008) Isoleucine and valine supplementation of a low-protein corn-wheat-soybean meal-based diet for piglets: growth performance and nitrogen balance. J Anim Sci 86, 29362941.
28. Gaster, M, Franch, J, Staehr, P, et al. (2000) Induction of GLUT-1 protein in adult human skeletal muscle fibers. Am J Physiol Endocr M 279, E1191E1195.
29. Santos, J, Benite‐Ribeiro, S, Queiroz, G, et al. (2012) The effect of age on glucose uptake and GLUT1 and GLUT4 expression in rat skeletal muscle. Cell Biochem Funct 30, 191197.
30. Tessari, P, Inchiostro, S, Biolo, G, et al. (1985) Hyperaminoacidaemia reduces insulin-mediated glucose disposal in healthy man. Diabetologia 28, 870872.
31. Krebs, M, Krssak, M, Bernroider, E, et al. (2002) Mechanism of amino acid-induced skeletal muscle insulin resistance in humans. Diabetes 51, 599605.
32. Li, C, Najafi, H, Daikhin, Y, et al. (2003) Regulation of leucine-stimulated insulin secretion and glutamine metabolism in isolated rat islets. J Biol Chem 278, 28532858.
33. Lund, S, Holman, G, Schmitz, O, et al. (1995) Contraction stimulates translocation of glucose transporter GLUT4 in skeletal muscle through a mechanism distinct from that of insulin. Proc Natl Acad Sci U S A 92, 58175821.
34. Koivisto, U-M, Martinez-Valdez, H, Bilan, P, et al. (1991) Differential regulation of the GLUT-1 and GLUT-4 glucose transport systems by glucose and insulin in L6 muscle cells in culture. J Biol Chem 266, 26152621.
35. Chen, HC, Bandyopadhyay, G, Sajan, MP, et al. (2002) Activation of the ERK pathway and atypical protein kinase C isoforms in exercise-and aminoimidazole-4-carboxamide-1-β-d-riboside (AICAR)-stimulated glucose transport. J Biol Chem 277, 2355423562.
36. Wu, G (2009) Amino acids: metabolism, functions, and nutrition. Amino Acids 37, 117.
37. Wu, G (1998) Intestinal mucosal amino acid catabolism. J Nutr 128, 12491252.
38. Ferraris, RP (2001) Dietary and developmental regulation of intestinal sugar transport. Biochem J 360, 265276.
39. Dyer, J, Hosie, K & Shirazi-Beechey, S (1997) Nutrient regulation of human intestinal sugar transporter (SGLT1) expression. Gut 41, 5659.
40. Margolskee, RF, Dyer, J, Kokrashvili, Z, et al. (2007) T1R3 and gustducin in gut sense sugars to regulate expression of Na+-glucose cotransporter 1. Proc Natl Acad Sci U S A 104, 1507515080.
41. Dyer, J, Daly, K, Salmon, K, et al. (2007) Intestinal glucose sensing and regulation of intestinal glucose absorption. Biochem Soc Trans 35, 11911194.
42. Mace, OJ, Lister, N, Morgan, E, et al. (2009) An energy supply network of nutrient absorption coordinated by calcium and T1R taste receptors in rat small intestine. J Physiol 587, 195210.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed