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Effect of pharmacological doses of ascorbic acid on the hepatic microsomal haemoproteins in the guinea-pig

Published online by Cambridge University Press:  09 March 2007

Judith L. Sutton ,
T. K. Basu  and
J. W. T. Dickerson
Judith L. Sutton*
Affiliation:
Division of Nutrition and Food Science, Department of Biochemistry, University of Surrey, Guildford GU2 5XH, Surrey
T. K. Basu*
Affiliation:
Division of Nutrition and Food Science, Department of Biochemistry, University of Surrey, Guildford GU2 5XH, Surrey
J. W. T. Dickerson
Affiliation:
Division of Nutrition and Food Science, Department of Biochemistry, University of Surrey, Guildford GU2 5XH, Surrey
*
* Foods and Nutrition Division, Faculty of Home Economics, The University of Alberta, Edmonton, Canada.
* Foods and Nutrition Division, Faculty of Home Economics, The University of Alberta, Edmonton, Canada.
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Abstract

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1. Ascorbic acid deficiency results in a reduction in the activity of the hepatic mixed function oxidase systems in the guinea-pig. In this study, male Dunkin-Hartley guinea-pigs were given 0, 50, 100, 200 or 300 mg ascorbic acid/d in two equal doses in buffered sucrose solution (200 g/l) for 4 d. Controls received an equal volume of sucrose solution.

2. A dose of 50 mg ascorbic acid/d resulted in a significant rise in the specific activities of both cytochromes P-450 and b5. At doses of 200 and 300 mg ascorbic acid/d the concentration of both haemoproteins was significantly lower than the control values. These effects were mirrored by total microsomal haem concentration.

3. These results suggest that when given in large doses, ascorbic acid ceases to act simply as a vitamin and should be considered a drug competing for substrates and cofactors with co-administered drugs and endogenous substrates such as cholesterol.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 49 , Issue 1 , January 1983 , pp. 27 - 33
Copyright
Copyright © The Nutrition Society 1983

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