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Effect of graded calcium supplementation in low-nutrient density feed on tibia composition and bone turnover in meat ducks

  • Huaiyong Zhang (a1), Qiufeng Zeng (a1), Shiping Bai (a1), Jianping Wang (a1), Xuemei Ding (a1), Yue Xuan (a1), Zhuowei Su (a1) and Keying Zhang (a1)...

Abstract

Both genetic selection and increasing nutrient density for improving growth performance had inadvertently increased leg problems of meat ducks, which adversely affects animal welfare. We hypothesised that slowing weight gain with improving tibia quality probably enhanced tibial mechanical properties and alleviated leg deformities. Therefore, the present study aimed to evaluate the effect of graded Ca supplementation in a low-nutrient density (LND) diet on tibia composition and bone turnover in meat ducks. A total of 720 15-d-old male meat ducks were randomly assigned and fed a standard nutrient density positive control (PC) diet containing 0·9 % Ca, and four LND diets with 0·5, 0·7, 0·9 and 1·1 % Ca, respectively. Ducks fed the 0·5 % Ca LND diet and the PC diet had higher incidence of tibial dyschondroplasia (TD). When compared with the 0·5 % Ca LND diet, LND diets with ≥0·7 % Ca significantly improved tibia composition, microarchitecture and mechanical properties, and consequently decreased the incidence of TD. Furthermore, LND diets with ≥0·7 % Ca increased osteocyte-specific gene mRNA expression, blocked the expression of osteoblast differentiation marker genes including osteocalcin, collagenase-1 and alkaline phosphatase (ALP), and also decreased the expression of osteoclast differentiation genes, such as vacuolar-type H+-ATPase, cathepsin K and receptor activator of NF-κB. Meanwhile bone markers such as serum ALP, osteocalcin (both osteoblast markers) and tartrate-resistant acid phosphatase (an osteoclast marker) were significantly decreased in at least 0·7 % Ca treated groups. These findings indicated that LND diets with ≥0·7 % Ca decreased bone turnover, which subsequently increased tibia quality for 35-d-old meat ducks.

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Corresponding author

*Corresponding author: Professor K. Zhang, fax +86 8352 885 630, email keying@sicau.edu.cn

References

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1. Cherry, P & Morris, TR (2008) Domestic Duck Production: Science and Practice. Cambridge.
2. Waldenstedt, L (2006) Nutritional factors of importance for optimal leg health in broilers: a review. Anim Feed Sci Technol 126, 291307.
3. Brickett, KE, Dahiya, JP, Classen, HL, et al. (2007) The impact of nutrient density, feed form, and photoperiod on the walking ability and skeletal quality of broiler chickens. Poult Sci 86, 21172125.
4. Zhao, JP, Chen, JL, Zhao, GP, et al. (2009) Live performance, carcass composition, and blood metabolite responses to dietary nutrient density in two distinct broiler breeds of male chickens. Poult Sci 88, 25752584.
5. Fan, HP, Xie, M, Wang, WW, et al. (2008) Effects of dietary energy on growth performance and carcass quality of white growing Pekin ducks from two to six weeks of age. Poult Sci 87, 11621164.
6. Williams, B, Waddington, D, Murray, DH, et al. (2004) Bone strength during growth: influence of growth rate on cortical porosity and mineralization. Calcif Tissue Int 74, 236245.
7. Elliot, MA & Edwards, HM (1994) Effect of genetic strain, calcium, and feed withdrawal on growth, tibial dyschondroplasia, plasma 1,25-dihydroxycholecalciferol, and plasma 25-hydroxycholecalciferol in sixteen-day-old chickens. Poult Sci 73, 509519.
8. Rath, NC, Huff, GR, Huff, WE, et al. (2000) Factors regulating bone maturity and strength in poultry. Poult Sci 79, 10241032.
9. Abdulla, NR, Loh, TC, Akit, H, et al. (2017) Effects of dietary oil sources, calcium and phosphorus levels on growth performance, carcass characteristics and bone quality of broiler chickens. J Appl Anim Res 45, 423429.
10. Xie, M, Wang, SX, Hou, SS, et al. (2009) Interaction between dietary calcium and non-phytate phosphorus on growth performance and bone ash in early White Pekin ducklings. Anim Feed Sci Technol 151, 161166.
11. Onyango, EM, Hester, PY, Stroshine, R, et al. (2003) Bone densitometry as an indicator of percentage tibia ash in broiler chicks fed varying dietary calcium and phosphorus levels. Poult Sci 82, 17871791.
12. Venäläinen, E, Valaja, J & Jalava, T. (2006) Effects of dietary metabolizable energy, calcium and phosphorus on bone mineralisation, leg weakness and performance of broiler chickens. Br Poult Sci 47, 301310.
13. Walk, CL, Addo-Chidie, EK, Bedford, MR, et al. (2012) Evaluation of a highly soluble calcium source and phytase in the diets of broiler chickens. Poult Sci 91, 22552263.
14. Aquino-Martínez, R, Artigas, N, Gámez, B, et al. (2017) Extracellular calcium promotes bone formation from bone marrow mesenchymal stem cells by amplifying the effects of BMP-2 on SMAD signalling. PLOS ONE 12, e0178158.
15. Yoo, HS, Kim, GJ, Song, DH, et al. (2017) Calcium supplement derived from Gallus domesticus promotes BMP-2/RUNX2/SMAD5 and suppresses TRAP/RANK expression through MAPK signaling activation. Nutrients 9, E504.
16. China Agricultural Industry Standards (2012) Nutrient Requirement of Meat-type Ducks (NY/T 2122-2012). Beijing: China Agricultural Industry Standards.
17. Edwards, HM & Veltmann, JR (1983) The role of calcium and phosphorus in the etiology of tibial dyschondroplasia in young chicks. J Nutr 113, 15681575.
18. Zhang, HY, Liao, H, Zeng, QF, et al. (2017) A study on the sternum growth and mineralization kinetic of meat duck from 35 to 63 days of age. Poult Sci 96, 41034115.
19. Zhang, H, Gilbert, ER, Pan, S, et al. (2016) Dietary iron concentration influences serum concentrations of manganese in rats consuming organic or inorganic sources of manganese. Br J Nutr 115, 585593.
20. Vandesompele, J, De Preter, K, Pattyn, F, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3, RESEARCH0034.
21. Shim, M, Karnuah, AB, Mitchell, AD, et al. (2012) The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers. Poult Sci 91, 17901795.
22. Schmidt-Nielsen, K (1984) Scaling: Why is Animal Size so Important?. Cambridge.
23. Tickle, PG, Paxton, H, Rankin, JW, et al. (2014) Anatomical and biomechanical traits of broiler chickens across ontogeny. Part I. Anatomy of the musculoskeletal respiratory apparatus and changes in organ size. PeerJ 2, e432.
24. Coto, C, Yan, F, Cerrate, S, et al. (2008) Effects of dietary levels of calcium and nonphytate phosphorus in broiler starter diets on live performance, bone development and growth plate conditions in male chicks fed a wheat-based diet. Int J Poult Sci 7, 101109.
25. Theobald, HE (2005) Dietary calcium and health. Nutr Bull 30, 237277.
26. National Research Council (1994) Nutrient Requirements of Poultry, 9th rev. ed. Washington, DC: National Academies Press.
27. Iwamoto, J, Takeda, T, Sato, Y, et al. (2004) Response of cortical and cancellous bones to mild calcium deficiency in young growing female rats: a bone histomorphometry study. Exp Anim 53, 347354.
28. Lv, WT, Yang, YH, Ma, LQ, et al. (2014) Ipriflavone reverses the adverse effects of a low-calcium diet on the histology of the tibia in caged layers. Br Poult Sci 55, 207214.
29. Cepelak, I & Cvoriscec, D (2009) Biochemical markers of bone remodeling – review. Biochem Med 19, 1735.
30. Ross, PD, Kress, BC, Parson, RE, et al. (2009) Serum bone alkaline phosphatase and calcaneus bone density predict fractures: a prospective study. Osteoporos Int 11, 7682.
31. Kanbur, NO, Derman, O, Sen, TA, et al. (2000) Osteocalcin. A biochemical marker of bone turnover during puberty. Int J Adolesc Med Health 14, 235244.
32. Li, J, Hao, L, Wu, J, et al. (2016) Linarin promotes osteogenic differentiation by activating the BMP-2/RUNX2 pathway via protein kinase a signaling. Int J Mol Med 37, 901910.
33. Halleen, JM, Ylipahkala, H, Alatalo, SL, et al. (2000) Serum tartrateresistant acid phosphatase 5b, but not 5a, correlates with other markers of bone turnover and bone mineral density. Calcif Tissue Int 71, 2025.
34. Peterson, CA, Eurell, JA, Kelley, KW, et al. (1995) Bone composition and histological analysis of young and aged rats fed diets of varied calcium bioavailability. J Am Coll Nutr 14, 278285.
35. Bonewald, LF (2007) Osteocytes as dynamic multifunctional cells. Ann N Y Acad Sci 1116, 281290.
36. Xiong, Y, Yang, HJ, Feng, J, et al. (2009) Effects of alendronate on the proliferation and osteogenic differentiation of MG-63 cells. J Int Med Res 37, 407416.
37. An, S, Gao, Y, Ling, J, et al. (2012) Calcium ions promote osteogenic differentiation and mineralization of human dental pulp cells: implications for pulp capping materials. J Mater Sci Mater Med 23, 789795.
38. Everts, V, Delaissé, JM, Korper, W, et al. (1992) Degradation of collagen in the bone-resorbing compartment underlying the osteoclast involves both cysteine-proteinases and matrix metalloproteinases. J Cell Physiol 150, 221231.
39. Riihonen, R, Supuran, CT, Parkkila, S, et al. (2007) Membrane-bound carbonic anhydrases in osteoclasts. Bone 40, 10211031.
40. Fujisaki, K, Tanabe, N, Suzuki, N, et al. (2007) Receptor activator of NF-kappaB ligand induces the expression of carbonic anhydrase II, cathepsin K, and matrix metalloproteinase-9 in osteoclast precursor RAW264.7 cells. Life Sci 80, 13111318.
41. Shin, MM, Kim, YH, Kim, SN, et al. (2003) High extracellular Ca2+ alone stimulates osteoclast formation but inhibits in the presence of other osteoclastogenic factors. Exp Mol Med 35, 167174.
42. Sapkota, M, Li, L, Choi, H, et al. (2015) A inhibits osteoclastogenic differentiation in RAW 264.7 cells via Akt and ERK signaling pathways. Eur J Pharmacol 769, 100109.
43. Yamamoto, Y, Udagawa, N, Matsuura, S, et al. (2006) Osteoblasts provide a suitable microenvironment for the action of receptor activator of nuclear factor-kappaB ligand. Endocrinology 147, 33663374.
44. Mentaverri, R, Yano, S, Chattopadhyay, N, et al. (2006) The calcium sensing receptor is directly involved in both osteoclast differentiation and apoptosis. FASEB J 20, 25622564.
45. Kemi, VE, Karkkainen, MU, Rita, HJ, et al. (2010) Low calcium:phosphorus ratio in habitual diets affects serum parathyroid hormone concentration and calcium metabolism in healthy women with adequate calcium intake. Br J Nutr 103, 561568.
46. Tatara, MR, Majcher, P, Krupski, W, et al. (2004) Volumetric bone density, morphological and mechanical properties of femur and tibia in farm turkeys with leg deformities. Bull Vet Inst Pulawy 48, 169172.

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