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The effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig

  • Suzanne M. Hodgkinson (a1), Paul J. Moughan (a1), Gordon W. Reynolds (a1) and Kerry A. C. James (a2)

Abstract

The aim of the present study was to determine whether dietary peptide concentration had an effect on endogenous ileal amino acid flow in the growing pig. Eight 33 kg live weight entire male pigs had post-valve T-caecum (PVTC) cannulas surgically implanted for the collection of ileal digesta. The pigs were fed twice daily at 100 g/kg metabolic body weight per d and were given diets containing enzyme-hydrolysed casein (EHC) at 0, 50, 100 and 200 g/kg in a Latin-square design. A basal casein-based diet was fed to the pigs for 6 d periods between receiving the experimental diets. The pigs received the experimental diets for 8 d periods, with continuous collection of digesta for 24 h on each of the fifth and eighth days. The endogenous ileal amino acid flows were determined with reference to recovery of the marker, Cr, directly for pigs receiving the protein-free diet or after centrifugation and ultrafiltration (10 000 Da molecular mass cut-off) for pigs on the EHC-based diets. Mean endogenous ileal N flows were 1753, 1948, 2851 and 5743 μg/g DM intake when the pigs received diets containing 0, 50, 100 and 200 g EHC/kg respectively. There was a significant (P < 0·05) effect of dietary peptide concentration on the endogenous ileal flows of N and all of the amino acids, with an increase in endogenous ileal amino flow with increasing dietary EHC concentration.

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Copyright

Corresponding author

*Corresponding author: Dr Suzanne Hodgkinson, fax +64 6 350 5671, email S.M.Hodgkinson@massey.ac.nz

References

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Bell, JM, Shires, A and Keith, MO (1983) Effect of hull and protein contents of barley on protein and energy digestibility and feeding value for pigs. Canadian Journal of Animal Science 63, 201211.
Butts, CA, Moughan, PJ and Koolaard, J (1998) The effect of dietary peptide intake on endogenous ileal lysine excretion in the growing rat Proceedings of the Nutrition Society of New Zealand 23,(In the Press).
Butts, CA, Moughan, PJ and Smith, WC (1991) Endogenous amino acid flow at the terminal ileum of the rat determined under conditions of peptide alimentation. Journal of the Science of Food and Agriculture 55, 175187.
Butts, CA, Moughan, PJ and Smith, WC (1992) Protein nitrogen, peptide nitrogen and free amino acid nitrogen in endogenous digesta nitrogen at the terminal ileum of the rat. Journal of the Science of Food and Agriculture 59, 291298.
Butts, CA, Moughan, PJ, Smith, WC and Car, DH (1993) Endogenous lysine and other amino acid flows at the terminal ileum of the growing pig (20 kg bodyweight): the effect of protein-free, synthetic amino acid, peptide and protein alimentation. Journal of the Science of Food and Agriculture 61, 3140.
Butts, CA. Moughan, PJ, Smith, WC, Reynolds, GW and Garrick, DJ (1993) The effect of food dry matter intake on endogenous ileal amino acid excretion determined under peptide alimentation in the 50 kg liveweight pig. Journal of the Science of Food and Agriculture 62, 235243.
Costigan, P and Ellis, KJ (1987) Analysis of faecal chromium derived from controlled release marker devices. New Zealand Journal of Technology 3, 8992.
Darragh, AJ, Moughan, PJ, Rutherfurd, SM & Boisen, S (1995) Amino acid availability in feedstuffs for the growing pig. In Recent Advances in Animal Nutrition in Australia 1995, pp. 2329 [Rowe, JB, and Nolan, JV, editors]. Armidale: University of New England.
Darragh, AJ, Moughan, PJ and Smith, WC (1990) The effect of amino acid and peptide alimentation on the determination of endogenous amino acid flow at the terminal ileum of the rat. Journal of the Science of Food and Agriculture 51, 4756.
de Lange, CFM, Sauer, WC, Mosenthin, R and Souffrant, WB (1989) The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces in pigs. Journal of Animal Science 67, 745754.
de Lange, CFM, Sauer, WC and Souffrant, W (1989) The effect of protein status of the pig on the recovery and amino acid composition of endogenous protein in digesta collected from the distal ileum. Journal of Animal Science 67, 755762.
de Lange, CFM, Souffrant, WB and Sauer, WC (1990) Real ileal protein and amino acid digestibilities in feedstuffs for growing pigs as determined with the 15N-isotope dilution technique. Journal of Animal Science 68, 409418.
den Hartog, L, Avan Leeuwen, P, Huisman, J, Zandstra Tvan Heugten, E, van Ommeren, HJ & van Kleef, D (1988) Comparison of ileal digestibility data obtained from pigs provided with a different type of cannula. In Digestive Physiology in the Pig. Proceedings of the 4th International Seminar, pp. 275–82 [Buraczewska, L, Buraczewski, S, Pastuszewska, B and Zebrowska, T, editors]. Joblonna, Poland: Institute of Animal Physiology and Nutrition.
Donkoh, A and Moughan, PJ (1994) The effect of dietary crude protein content on apparent and true ileal nitrogen and amino acid digestibilities. British Journal of Nutrition 72, 5968.
Donkoh, A, Moughan, PJ and Morel, PCH (1995) Comparison of methods to determine the endogenous amino acid flow at the terminal ileum of the growing rat. Journal of the Science of Food and Agriculture 67, 359366.
Ehle, FR, Jeraci, JL, Robertson, JE and van Soest, PJ (1982) The influence of dietary fibre on digestibility, rate of passage and gastrointestinal fermentation in pigs. Journal of Animal Science 55, 1071.
Fauconneau, G & Michel, MC (1970) The role of the gastrointestinal tract in the regulation of protein metabolism. In Mammalian Protein Metabolism, vol. 4, pp. 481522 [Munro, HN, editor]. London: Academic Press Inc.
Furuya, S and Kaji, Y (1989) Estimation of the true ileal digestibility of amino acids and nitrogen from their apparent values for growing pigs. Animal Feed Science and Technology 26, 271285.
Furuya, S and Kaji, Y (1992) The effects of feed intake and purified cellulose on the endogenous ileal amino acid flow in growing pigs. British Journal of Nutrition 68, 463472.
Hagemeister, H and Erbersdobler, H (1985) Chemical labelling of dietary protein by transformation of lysine to homoarginine: a new technique to follow intestinal digestion and absorption. Proceedings of the Nutrition Society 44, 133A.
Hendriks, WH, Moughan, PJ and Tarttelin, MF (1996) Gut endogenous nitrogen and amino acid excretions in adult domestic cats fed a protein-free or an enzymatically hydrolyzed casein–based diet. Journal of Nutrition 126, 955962.
Kohler, T, den Hartog, LA, Huisman, J, Mosenthin, R and Verstegen, MWA (1991) Digestibility measurements in pigs by using post-valve T-caecum cannulation or end-to-side ileo-rectal anastomosis. Journal of Animal Physiology and Animal Nutrition 66, 278286.
Kohler, T, Huisman, Jden Hartog, LA and Mosenthin, R (1990) Comparison of different digesta collection methods to determine the apparent digestibilities of the nutrients at the terminal ileum in pigs. Journal of the Science of Food and Agriculture 53, 465475.
Kohler, T, Mosenthin, R, Verstegen, MWA, Huisman, Jden Hartog, LA and Ahrens, F (1992) Effect of ileo-rectal anastomosis and post-valve T-caecum cannulation on growing pigs. 1. Growth performance, N-balance and intestinal adaptation. British Journal of Nutrition 68, 293303.
Kohler, T, Verstegen, MWA, Mosenthin, R, Wensing, Tden Hartog, LA and Huisman, J (1992) Effect of ileo-rectal anastomosis and post-valve T-caecum cannulation on growing pigs. 2. Blood variables and mineral balances. British Journal of Nutrition 68, 305315.
Leterme, P, Monmart, T, Théwis, A and Morandi, P (1996) Effect of oral and parenteral N nutrition vs N-free nutrition on the endogenous amino acid flow at the ileum of the pig. Journal of the Science of Food and Agriculture 71, 265271.
Leterme, P, Pirard, L and Théwis, A (1992) A note on the effect of wood cellulose level in protein-free diets on the recovery and amino acid composition of endogenous protein collected from the ileum in pigs. Animal Production 54, 163165.
Millward, DJ, Garlock, PJ, James, WPT, Sender, PM & Waterlow, JC (1976) Protein turnover. In Protein Metabolism and Nutrition, pp. 4969 [Cole, DJA, Boorman, KN, Buttery, PJ, Lewis, D, Neale, RJ and Swan, H, editors]. London: Butterworths.
Moughan, PJ, Darragh, AJ, Smith, WC and Butts, CA (1990) Perchloric and trichloroacetic acids as precipitants of protein in endogenous ileal digesta from the rat. Journal of the Science of Food and Agriculture 52, 1321.
Moughan, PJ and Rutherfurd, SM (1990) Endogenous flow of total lysine and other amino acids at the distal ileum of the protein- or peptide-fed rat: the chemical labelling of gelatine protein by transformation of lysine to homoarginine. Journal of the Science of Food and Agriculture 52, 179192.
Moughan, PJ and Schuttert, G (1991) Composition of nitrogen-containing fractions in digesta from the distal ileum of pigs fed a protein-free diet. Journal of Nutrition 121, 15701574.
Moughan, PJ, Schuttert, G and Leenaars, M (1992) Endogenous amino acid flow in the stomach and small intestine of the young growing pig. Journal of the Science of Food and Agriculture 60, 437442.
Mroz, Z, Bakker, GCM, Jongbloed, AW, Dekker, RA, Jongbloed, R and van Beers, A (1996) Apparent digestibility of nutrients in diets with different energy density, as estimated by direct and marker methods for pigs with or without ileo-caecal cannulas. Journal of Animal Science 74, 403412.
Rowan, AM, Moughan, PJ, Wilson, MN, Maher, K and Tasman-Jones, C (1994) Comparison of the ileal and faecal digestibility of dietary amino acids in adult humans and evaluation of the pig as a model animal for digestion studies in man. British Journal of Nutrition 71, 2942.
Rutherfurd, SM and Moughan, PJ (1990) Guanidination of lysine in selected dietary proteins. Journal of Agricultural and Food Chemistry 38, 209211.
Sauer, WC, Just, AJørgensen, HH, Fekadu, M and Eggum, BO (1980) The influence of diet composition on the apparent digestibility of crude protein and amino acids at the terminal ileum and overall in pigs. Acta Agricultura Scandinavica 30, 449459.
Schneeman, BO (1982) Digestive enzyme activities from the pancreas in response to diet. In Physiologie Digestive chez le Porc, pp. 125131 [Laplace, JP, Corring, T and Rerat, A, editors]. Paris: Institut National de la Recherche Agronomique.
Schulze, H, Butts, CA, Moughan, PJ and Verstegen, MWA (1995) The 15N-isotope dilution method for determining ileal endogenous nitrogen excretion in the young (10 kg liveweight) pig. Journal of the Science of Food and Agriculture 69, 4150.
Snedecor, GW & Cochran, WG (1980) Statistical Methods, 7th ed. Ames, IA: Iowa State University Press.
Snook, JT (1973) Protein digestion. World Review of Nutrition and Dietetics 18, 121176.
Souffrant, WB (1991) Endogenous nitrogen losses during digestion in pigs. In Proceedings of the Vth International Symposium on Digestive Physiology in Pigs, pp. 147166 [Verstegen, MWA, Huisman, J and den Hartog, LA, editors]. Wageningen: Pudoc.
Souffrant, WB, Février, CLaplace, JP & Hennig, U (1997) Comparison of the methods to estimate ileal endogenous nitrogen and amino acids in piglets. In Proceedings of the VIIth International Symposium on Digestive Physiology in Pigs. EAAP Publication no. 88, pp. 408412 [Laplace, JP, Février, C and Barbeau, A, editors]. Saint Malo: Institut National de la Recherche Agronomique.
Souffrant, WB, Kohler, R & Beghardt, G (1982) Détermination de l'azote endogène dans les contenus digestifs par la technique isotopique (15N) (Determination of endogenous nitrogen in the digestive contents by the isotope technique (15N)). In Physiologie Digestive chez le Porc. Les Colloques de l'INR no. 12, pp. 176187 [Laplace, JP, Corring, T and Rerat, A, editors]. Paris: Institut National de la Recherche Agronomique.
Taverner, MR (1979) Ileal availability for pigs of amino acids in cereal grains. PhD Thesis, University of New England, Australia.
Taverner, MR, Hume, ID and Farrell, DJ (1981) Availability to pigs of amino acids in cereal grains. I. Endogenous levels of amino acids in ileal digesta and faeces of pigs given cereal diets. British Journal of Nutrition 46, 149158.
van Leeuwen, P, van Kleef, DJ, van Kempen, GJM, Huisman, J and Verstegen, MWA (1991) The post-valve T-caecum cannulation technique in pigs applicated (sic) to determine the digestibility of amino acid in maize, groundnut and sunflower seed. Journal of Animal Physiology and Animal Nutrition 65, 183193.
van Leeuwen, P, Veldman, A, Boisen, S, Deuring, K, van Kempen, GJM, Derksen, GB, Verstegen, MWA and Schaafsma, G (1996) Apparent ileal dry matter and crude protein digestibility of rations fed to pigs and determined with the use of chromic oxide (Cr2O3) and acid-insoluble ash as digestive markers. British Journal of Nutrition 76, 551562.

Keywords

The effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig

  • Suzanne M. Hodgkinson (a1), Paul J. Moughan (a1), Gordon W. Reynolds (a1) and Kerry A. C. James (a2)

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