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Diosmectite–zinc oxide composite improves intestinal barrier function, modulates expression of pro-inflammatory cytokines and tight junction protein in early weaned pigs

  • Caihong Hu (a1), Juan Song (a1), Yali Li (a1), Zhaoshuang Luan (a1) and Kang Zhu (a1)...

Abstract

The study evaluated whether feeding diosmectite–ZnO composite (DS-ZnO) at 500 mg Zn/kg to early weaned pigs would alleviate the weaning-related intestinal disorders as a substitute for high concentration of ZnO (2250 mg Zn/kg). The pigs weaned at an age of 21 ± 1 d were allotted to four treatments groups as follows: (1) control; (2) DS-ZnO, 500 mg Zn/kg diet; (3) ZnO, 2250 mg Zn/kg diet; and (4) mixture of 2·0 g DS/kg and 500 mg Zn/kg from ZnO (equal amount of DS and ZnO in the DS-ZnO treatment group). The results showed that, compared with the control on days 7 and 14 post-weaning, addition of DS-ZnO at 500 mg Zn/kg improved (P< 0·05) daily gain and feed intake, decreased (P< 0·05) post-weaning scour scores, increased (P< 0·05) jejunal villus height and the ratio of villus height and crypt depth, decreased (P< 0·05) jejunal paracellular permeability of fluorescein isothiocyanate dextran 4 kDa and up-regulated (P< 0·05) tight junction protein expression of occludin, claudin-1 and zonula occludens-1 in jejunal mucosa. The mRNA levels of TNF-α, IL-6 and interferon-γ (IFN-γ) on day 7 post-weaning were also decreased (P< 0·05). The piglets fed DS-ZnO at 500 mg Zn/kg did not differ in the above parameters from those fed ZnO at 2250 mg Zn/kg, while they had better performance than those fed the mixture of DS and ZnO. Supplementation with DS-ZnO at 500 mg Zn/kg was effective in alleviating diarrhoea, barrier dysfunction and inflammatory cytokine expression and up-regulating tight junction protein expression.

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Corresponding author

*Corresponding author: Dr C. H. Hu, email chhu@zju.edu.cn

References

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1Pié, S, Lallès, JP, Blazy, F, et al. (2004) Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets. J Nutr 134, 641647.
2Wijtten, PJA, Meulen, J & Verstegen, MWA (2011) Intestinal barrier function and absorption in pigs after weaning: a review. Br J Nutr 105, 967981.
3Kim, JC, Hansen, CF, Mullana, BP, et al. (2012) Nutrition and pathology of weaner pigs: nutritional strategies to support barrier function in the gastrointestinal tract. Anim Feed Sci Technol 173, 316.
4Sargeant, HR, Miller, HM & Shaw, MA (2011) Inflammatory response of porcine epithelial IPEC J2 cells to enterotoxigenic E. coli infection is modulated by zinc supplementation. Mol Immunol 48, 21132121.
5Patel, A, Mamtani, M, Dibley, MJ, et al. (2010) Therapeutic value of zinc supplementation in acute and persistent diarrhea: a systematic review. PLoS ONE 5, e10386.
6Hahn, JD & Baker, DH (1993) Growth and plasma zinc responses of young pigs fed pharmacologic levels of zinc. J Anim Sci 71, 30203024.
7Poulsen, HD & Larsen, T (1995) Zinc excretion and retention in growing pigs fed increasing levels of zinc oxide. Livest Prod Sci 43, 235242.
8Vaiana, CA, Leonard, MK, Drummy, LF, et al. (2011) Epidermal growth factor: layered silicate nanocomposites for tissue regeneration. Biomacromolecules 12, 31393146.
9Zheng, JP, Luan, L, Wang, HY, et al. (2007) Study on ibuprofen/montmorillonite intercalation composites as drug release system. Appl Clay Sci 36, 297301.
10Joshi, GV, Patel, HA, Bajaj, HC, et al. (2009) Intercalation and controlled release of vitamin B6 from montmorillonite–vitamin B6 hybrid. Colloid Polym Sci 287, 10711076.
11Aguzzi, C, Cerezo, P, Viseras, C, et al. (2007) Use of clays as drug delivery systems: possibilities and limitations. Appl Clay Sci 36, 2236.
12Droy-Lefaix, MT, Drouet, Y & Schatz, B (1985) Sodium glycodeoxycholate and spinability of gastrointestinal mucus-protective effect of smectite. Gastroenterology 88, 13691369.
13Gonzalez, R, Sanchez de Medina, F, Martinez-Augustin, O, et al. (2004) Anti-inflammatory effect of diosmectite in hapten-induced colitis in the rat. Br J Pharmacol 141, 951960.
14Dupont, C & Vernisse, B (2009) Anti-diarrheal effects of diosmectite in the treatment of acute diarrhea in children: a review. Pediatr Drugs 11, 8999.
15Song, M, Liu, Y, Soares, JA, et al. (2012) Dietary clays alleviate diarrhea of weaned pigs. J Anim Sci 90, 345360.
16Sargeant, HR, McDowall, KJ, Miller, HM, et al. (2010) Dietary zinc oxide affects the expression of genes associated with inflammation: transcriptome analysis in piglets challenged with ETEC K88. Vet Immunol Immunopathol 137, 120129.
17Khaorapapong, N, Khumchoo, N & Ogawa, M (2011) Preparation of zinc oxide–montmorillonite hybrids. Mater Lett 65, 657660.
18Hu, CH, Song, J, You, ZT, et al. (2012) Zinc oxide–montmorillonite hybrid influences diarrhea, intestinal mucosal integrity, and digestive enzyme activity in weaned pigs. Biol Trace Elem Res 149, 190196.
19Hur, SG, Kim, TW, Hwang, SJ, et al. (2006) Heterostructured nanohybrid of zinc oxide–montmorillonite clay. J Phys Chem B 110, 15991604.
20National Research Council (1998) Nutrient Requirements of Swine, 10th ed.Washington, DC: National Academy Press.
21Hamard, A, Mazurais, D, Boudry, G, et al. (2010) A moderate threonine deficiency affects gene expression profile, paracellular permeability and glucose absorption capacity in the ileum of piglets. J Nutr Biochem 21, 914921.
22Liu, YL, Huang, JJ, Hou, YQ, et al. (2008) Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs. Br J Nutr 100, 552560.
23Livak, KJ & Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and 2− ΔΔC t method. Methods 25, 402408.
24Ewaschuk, JB, Murdoch, GK, Johnson, IR, et al. (2011) Glutamine supplementation improves intestinal barrier function in a weaned piglet model of Escherichia coli infection. Br J Nutr 106, 877877.
25SAS Institute, Inc. (2000) SAS/STAT User's Guide, Version 8.1. Cary, NC: SAS Institute, Inc.
26McCracken, BA, Spurlock, ME, Roos, MA, et al. (1999) Weaning anorexia may contribute to local inflammation in the piglet small intestine. J Nutr 129, 613619.
27Davis, ME, Brown, DC, Maxwell, CV, et al. (2004) Effect of phosphorylated mannans and pharmacological additions of zinc oxide on growth and immunocompetence of weanling pigs. J Anim Sci 82, 581587.
28Hollis, GR, Carter, SD, Cline, TR, et al. (2005) Effects of replacing pharmacological levels of dietary zinc oxide with lower dietary levels of various organic zinc sources for weanling pigs. J Anim Sci 83, 21232129.
29Montagne, L, Boudry, G, Favier, C, et al. (2007) Main intestinal markers associated with the changes in gut architecture and function in piglets after weaning. Br J Nutr 97, 4557.
30Smith, F, Clark, JE, Overman, BL, et al. (2010) Early weaning stress impairs development of mucosal barrier function in the porcine intestine. Am J Physiol Gastrointest Liver Physiol 298, G352G363.
31Sturniolo, GC, Fries, W, Mazzon, E, et al. (2002) Effect of zinc supplementation on intestinal permeability in experimental colitis. J Lab Clin Med 139, 311315.
32Zhang, BK & Guo, YM (2009) Supplemental zinc reduced intestinal permeability by enhancing occluding and zonula occludens protein-1 (ZO-1) expression in weaning piglets. Br J Nutr 102, 687693.
33Cummins, AG, Steele, TW, LaBrooy, JT, et al. (1988) Maturation of the rat small intestine at weaning: changes in the epithelial cell kinetics, bacterial flora, and mucosal immune activity. Gut 29, 16721679.
34Al-Sadi, R, Boivin, M & Ma, T (2009) Mechanism of cytokine modulation of epithelial tight junction barrier. Front Biosci 14, 27652778.
35Roselli, M, Finamore, A, Garaguso, I, et al. (2003) Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli. J Nutr 133, 40774082.
36Moeser, AJ, Ryan, KA, Nighot, PK, et al. (2007) Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs. Am J Physiol Gastrointest Liver Physiol 293, G413G421.
37Ou, DY, Li, DF, Cao, YH, et al. (2007) Dietary supplementation with zinc oxide decreases expression of the stem cell factor in the small intestine of weanling pigs. J Nutr Biochem 18, 820826.
38Li, X, Akhtar, S & Choudhry, MA (2012) Alteration in intestine tight junction protein phosphorylation and apoptosis is associated with increase in IL-18 levels following alcohol intoxication and burn injury. Biochim Biophys Acta 1822, 196203.
39Feed Database in China (2011) Table of Feed Composition and Nutritive Value in China, 22th ed.Beijing: China Feed.

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Diosmectite–zinc oxide composite improves intestinal barrier function, modulates expression of pro-inflammatory cytokines and tight junction protein in early weaned pigs

  • Caihong Hu (a1), Juan Song (a1), Yali Li (a1), Zhaoshuang Luan (a1) and Kang Zhu (a1)...

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