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Dietary secoisolariciresinol diglucoside and its oligomers with 3-hydroxy-3-methyl glutaric acid decrease vitamin E levels in rats

Published online by Cambridge University Press:  09 March 2007

Jan Frank ,
Christina Eliasson ,
Diane Leroy-Nivard ,
Alicja Budek ,
Torbjörn Lundh ,
Bengt Vessby ,
Per Åman  and
Afaf Kamal-Eldin
Jan Frank*
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences (SLU), Box 7051, S-750 07 Uppsala, Sweden
Christina Eliasson
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences (SLU), Box 7051, S-750 07 Uppsala, Sweden
Diane Leroy-Nivard
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences (SLU), Box 7051, S-750 07 Uppsala, Sweden
Alicja Budek
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences (SLU), Box 7051, S-750 07 Uppsala, Sweden
Torbjörn Lundh
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences (SLU), 750 07 Uppsala, Sweden
Bengt Vessby
Affiliation:
Department of Public Health and Caring Sciences and Unit for Clinical Nutrition Research, Uppsala University, 751 25 Uppsala, Sweden
Per Åman
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences (SLU), Box 7051, S-750 07 Uppsala, Sweden
Afaf Kamal-Eldin
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences (SLU), Box 7051, S-750 07 Uppsala, Sweden
*
*Corresponding author: fax +46 18 67 29 95, Email Jan.Frank@lmv.slu.se
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Abstract

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Secoisolariciresinol diglucoside (SDG) is an important dietary lignan that is found at very high levels in flaxseed (1–4%, w/w). Flaxseed lignans have received much research interest in recent years because of reported phyto-oestrogenic, anticarcinogenic, and anti-atherogenic effects. Previously, flaxseed feeding has been shown to decrease vitamin E concentrations in rats despite the antioxidant potential of SDG in vitro. Sesamin, a sesame lignan, on the other hand has been shown to increase vitamin E concentrations in rats. The aim of the present study was to investigate the effect of dietary SDG and its oligomers on vitamin E and cholesterol concentrations in rats. SDG was extracted from defatted flaxseed flour with a dioxane–ethanol mixture and purified by silica column chromatography. The major oligomers with 3-hydroxy-3-methyl glutaric acid, containing a high ratio of SDG to p−coumaric and ferulic acid glucosides, were purified from the extracts by reversed-phase liquid chromatography. When fed to rats at 0·1% in the diet for 27%, both SDG and its oligomers had no effect on animal performance but caused an increase in liver cholesterol and a 2-fold reduction in the levels of α- and γ-tocopherols in rat plasma and liver. It is notable that a phenolic antioxidant, such as SDG, causes a vitamin E-lowering effect in rats. This cannot be explained at present, but warrants further investigations with respect to the magnitude, mechanism, and significance of the observed effect for human nutrition.

Keywords

FlaxseedSecoisolariciresinol diglucosideEnterodiolVitamin ECholesterol
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 1 , July 2004 , pp. 169 - 176
Copyright
Copyright © The Nutrition Society 2004

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