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Dietary microbial phytase exerts mixed effects on the gut health of tilapia: a possible reason for the null effect on growth promotion

  • Jun Hu (a1) (a2), Chao Ran (a2), Suxu He (a2), Yanan Cao (a2), Bin Yao (a2), Yuantu Ye (a3), Xuezhen Zhang (a1) and Zhigang Zhou (a2)...


The present study evaluated the effects of dietary microbial phytase on the growth and gut health of hybrid tilapia (Oreochromis niloticus×Oreochromis aureus ♂), focusing on the effect on intestinal histology, adhesive microbiota and expression of immune-related cytokine genes. Tilapia were fed either control diet or diet supplemented with microbial phytase (1000 U/kg). Each diet was randomly assigned to four groups of fish reared in cages (3×3×2 m). After 12 weeks of feeding, weight gain and feed conversion ratio of tilapia were not significantly improved by dietary microbial phytase supplementation. However, significantly higher level of P content in the scales, tighter and more regular intestinal mucosa folds were observed in the microbial phytase group and the microvilli density was significantly increased. The adhesive gut bacterial communities were strikingly altered by microbial phytase supplementation (0·41<similarity coefficient<0·54). Stimulated intestinal inflammation and stress status were observed in the fish fed diet supplemented with microbial phytase, as indicated by the up-regulated intestinal expressions of the cytokine genes (tnf-α and tgf-β) and hsp70. In addition, the gut microvilli height was significantly decreased in the phytase group. These results indicate that dietary microbial phytase may exert mixed effects on hybrid tilapia, and can guide our future selection of phytases as aquafeed additives – that is, eliminating those that can stimulate intestinal inflammation.

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Corresponding author

* Corresponding authors: Y. Ye, fax +86 512 6588 0179, email; X. Zhang, fax +86 27 8728 2114, email; Z. Zhou, fax +86 10 8210 6054, email


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1. Fontainhas-Fernandes, A, Gomes, E, Reis-Henriques, MA, et al. (1999) Replacement of fish meal by plant proteins in the diet of Nile tilapia: digestibility and growth performance. Aquacult Int 7, 5767.
2. Mbahinzireki, GB, Dabrowski, K, Lee, KJ, et al. (2001) Growth, feed utilization and body composition of tilapia (Oreochromis sp.) fed with cottonseed meal-based diets in a recirculating system. Aquacult Nutr 7, 189200.
3. Jackson, LS, Li, MH & Robinson, EH (1996) Use of microbial phytase in channel catfish Ictalurus punctatus diets to improve utilization of phytate phosphorus. J World Aquacult Soc 27, 309313.
4. Reddy, NR, Sathe, SK & Salunkhe, DK (1982) Phytates in legumes and cereals. Adv Food Res 28, 192.
5. Kumar, V, Sinha, AK, Makkar, HPS, et al. (2012) Phytate and phytase in fish nutrition. J Anim Physiol Anim Nutr 96, 335364.
6. Cao, L, Wang, WM, Yang, CT, et al. (2007) Application of microbial phytase in fish feed. Enzyme Microb Technol 40, 497507.
7. Schafer, A, Koppe, WM, Meyerburgdorff, KH, et al. (1995) Effects of a microbial phytase on the utilization of native phosphorus by carp in a diet based on soybean-meal. Water Sci Technol 31, 149155.
8. Vielma, J, Lall, SP, Koskela, J, et al. (1998) Effects of dietary phytase and cholecalciferol on phosphorus bioavailability in rainbow trout (Oncorhynchus mykiss). Aquaculture 163, 309323.
9. Sajjadi, M & Carter, CG (2004) Dietary phytase supplementation and the utilisation of phosphorus by Atlantic salmon (Salmo salar L.) fed a canola-meal-based diet. Aquaculture 240, 417431.
10. Ai, QH, Mai, KS, Zhang, WB, et al. (2007) Effects of exogenous enzymes (phytase, non-starch polysaccharide enzyme) in diets on growth, feed utilization, nitrogen and phosphorus excretion of Japanese seabass, Lateolabrax japonicus . Comp Biochem Physiol A. Mol Integr Physiol 147, 502508.
11. Furuya, WM, Goncalves, GS, Furuya, VRB, et al. (2001) Phytase as feeding for Nile tilapia (Oreochromis niloticus). Performance and digestibility. Rev Bras Zootecn 30, 924929.
12. Liebert, F & Portz, L (2005) Nutrient utilization of Nile tilapia Oreochromis niloticus fed plant based low phosphorus diets supplemented with graded levels of different sources of microbial phytase. Aquaculture 248, 111119.
13. Cao, L, Yang, Y, Wang, WM, et al. (2008) Effects of pretreatment with microbial phytase on phosphorous utilization and growth performance of Nile tilapia (Oreochromis niloticus). Aquacult Nutr 14, 99109.
14. Van Weerd, JH, Khalaf, KA, Aartsen, FJ, et al. (1999) Balance trials with African catfish Clarias gariepinus fed phytase-treated soybean meal-based diets. Aquacult Nutr 5, 135142.
15. Papatryphon, E & Soares, JH (2001) The effect of phytase on apparent digestibility of four practical plant feedstuffs fed to striped bass, Morone saxatilis . Aquacult Nutr 7, 161167.
16. Debnath, D, Sahu, NP, Pal, AK, et al. (2005) Present scenario and future prospects of phytase in aquafeed – review. Asian Austral J Anim 18, 18001812.
17. Sugiura, SH, Gabaudan, J, Dong, FM, et al. (2001) Dietary microbial phytase supplementation and the utilization of phosphorus, trace minerals and protein by rainbow trout [Oncorhynchus mykiss (Walbaum)] fed soybean meal-based diets. Aquacult Res 32, 583592.
18. Oliva-Teles, A, Pereira, JP, Gouveia, A, et al. (1998) Utilisation of diets supplemented with microbial phytase by seabass (Dicentrarchus labrax) juveniles. Aquat Living Resour 11, 255259.
19. Vielma, J, Makinen, T, Ekholm, P, et al. (2000) Influence of dietary soy and phytase levels on performance and body composition of large rainbow trout (Oncorhynchus mykiss) and algal availability of phosphorus load. Aquaculture 183, 349362.
20. Nwanna, LC (2007) Effect of dietary phytase on growth, enzyme activities and phosphorus load of Nile tilapia (Oreochromis niloticus). J Franklin Inst 2, 972976.
21. Baruah, K, Sahu, NP, Pal, AK, et al. (2007) Dietary microbial phytase and citric acid synergistically enhances nutrient digestibility and growth performance of Labeo rohita (Hamilton) juveniles at sub-optimal protein level. Aquacult Res 38, 109120.
22. Omogbenigun, FO, Nyachoti, CM & Slominski, BA (2003) The effect of supplementing microbial phytase and organic acids to a corn-soybean based diet fed to early-weaned pigs. J Anim Sci 81, 18061813.
23. Portz, L & Liebert, F (2004) Growth, nutrient utilization and parameters of mineral metabolism in Nile tilapia Oreochromis niloticus (Linnaeus, 1758) fed plant-based diets with graded levels of microbial phytase. J Anim Physiol Anim Nutr (Berl) 88, 311320.
24. Liebert, F & Portz, L (2007) Different sources of microbial phytase in plant based low phosphorus diets for Nile tilapia Oreochromis niloticus may provide different effects on phytate degradation. Aquaculture 267, 292299.
25. Nelson, TS, Shieh, TR, Wodzinski, RJ, et al. (1971) Effect of supplemental phytase on the utilization of phytate phosphorus by chicks. J Nutr 101, 12891293.
26. Smulikowska, S, Czerwinski, J & Mieczkowska, A (2008) Effect of an organic acid blend and phytase added to a rapeseed cake-containing diet on performance, intestinal morphology, caecal microflora activity and thyroid status of broiler chickens. J Anim Physiol Anim Nutr (Berl) 94, 1523.
27. Metzler-Zebeli, BU, Vahjen, W, Baumgartel, T, et al. (2010) Ileal microbiota of growing pigs fed different dietary calcium phosphate levels and phytase content and subjected to ileal pectin infusion. J Anim Sci 88, 147158.
28. Verdegem, MCJ, Hilbrands, AD & Boon, JH (1997) Influence of salinity and dietary composition on blood parameter values of hybrid red tilapia, Oreochromis niloticus (Linnaeus) x O. mossambicus (Peters). Aquacult Res 28, 453459.
29. National Research Council (1993) Nutrient Requirements of Fish. Washington, DC: National Academies Press.
30. Engelen, AJ, van der Heeft, FC, Randsdorp, PH, et al. (1994) Simple and rapid determination of phytase activity. J AOAC Int 77, 760764.
31. Hughes, KP & Soares, JH (1998) Efficacy of phytase on phosphorus utilization in practical diets fed to striped bass Morone saxatilis . Aquacult Nutr 4, 133140.
32. Merrifield, DL, Dimitroglou, A, Bradley, G, et al. (2009) Soybean meal alters autochthonous microbial populations, microvilli morphology and compromises intestinal enterocyte integrity of rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 32, 755766.
33. Fagundes-Neto, U, De Martini-Costa, S, Pedroso, MZ, et al. (2000) Studies of the small bowel surface by scanning electron microscopy in infants with persistent diarrhea. Braz J Med Biol Res 33, 14371442.
34. Ringø, E (1993) ) The effect of chromic oxide (Cr2O3) on aerobic bacterial populations associated with the intestinal epithelial mucosa of Arctic charr, Salvelinus alpinus (L.). Can J Microbiol 39, 11691173.
35. He, SX, Zhou, ZG, Liu, YC, et al. (2009) Effects of dietary Saccharomyces cerevisiae fermentation product (DVAQUA (R)) on growth performance, intestinal autochthonous bacterial community and non-specific immunity of hybrid tilapia (Oreochromis niloticus female x O. aureus male) cultured in cages. Aquaculture 294, 99107.
36. Liu, YC, Zhou, ZG, Yao, B, et al. (2008) Effect of intraperitoneal injection of immunostimulatory substances on allochthonous gut microbiota of Atlantic salmon (Salmo salar L.) determined using denaturing gradient gel electrophoresis. Aquacult Res 39, 635646.
37. Zhou, ZG, He, SX, Liu, YC, et al. (2009) The effects of dietary yeast culture or short-chain fructo-oligosaccharides on the intestinal autochthonous bacterial communities in juvenile hybrid tilapia, Oreochromis niloticus female x Oreochromis aureus male. J World Aquacult Soc 40, 450459.
38. Liu, WS, Ren, PF, He, SX, et al. (2013) Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains. Fish Shellfish Immunol 35, 5462.
39. Wang, W, Zhou, Z, He, S, et al. (2010) Identification of the adherent microbiota on the gills and skin of poly-cultured gibel carp (Carassius auratus gibelio) and bluntnose black bream (Megalobrama amblycephala Yih). Aquac Res 41, 7283.
40. Ye, CX, Liu, YJ, Tian, LX, et al. (2006) Effect of dietary calcium and phosphorus on growth, feed efficiency, mineral content and body composition of juvenile grouper, Epinephelus coioides . Aquaculture 255, 263271.
41. Berntssen, MHG, Waagbø, R, Toften, H, et al. (2003) Effects of dietary cadmium on calcium homeostasis, Ca mobilization and bone deformities in Atlantic salmon (Salmo salar L.) parr. Aquacult Nutr 9, 175183.
42. Lall, SP (1989) The minerals. Fish Nutr 3, 219257.
43. Lall, SP & Lewis-McCrea, LM (2007) Role of nutrients in skeletal metabolism and pathology in fish – An overview. Aquaculture 267, 319.
44. Fuller, R, Perdigón, G, Fuller, R, et al. (2005) Gut flora, nutrition, immunity and health. Int J Dairy Technol 58, 238.
45. Kiarie, E, Romero, LF & Nyachoti, CM (2013) The role of added feed enzymes in promoting gut health in swine and poultry. Nutr Res Rev 26, 7188.
46. Vinolo, MA, Rodrigues, HG, Nachbar, RT, et al. (2011) Regulation of inflammation by short chain fatty acids. Nutrients 3, 858876.
47. Atarashi, K, Tanoue, T, Oshima, K, et al. (2013) Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota. Nature 500, 232236.
48. Iwama, GK, Thomas, PT, Forsyth, RB, et al. (1998) Heat shock protein expression in fish. Rev Fish Biol Fisher 8, 3556.
49. Xing, H, Li, S, Wang, X, et al. (2013) Effects of atrazine and chlorpyrifos on the mRNA levels of HSP70 and HSC70 in the liver, brain, kidney and gill of common carp (Cyprinus carpio L.). Chemosphere 90, 910916.
50. Morimoto, RI (1998) Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Gene Dev 12, 37883796.
51. Kudo, O, Fujikawa, Y, Itonaga, I, et al. (2002) Proinflammatory cytokine (TNFα/IL-1α) induction of human osteoclast formation. J Pathol 198, 220227.
52. Sano, C, Shimizu, T, Sato, K, et al. (2000) Effects of secretory leucocyte protease inhibitor on the production of the anti-inflammatory cytokines, IL-10 and transforming growth factor-beta (TGF-beta), by lipopolysaccharide-stimulated macrophages. Clin Exp Immunol 121, 7785.
53. Qiu, R, Croom, J, Ali, RA, et al. (2012) Direct fed microbial supplementation repartitions host energy to the immune system. J Anim Sci 90, 26392651.
54. Kazerani, HR & Shahsavani, D (2011) The effect of supplementation of feed with exogenous enzymes on the growth of common carp (Cyprinus carpio). Iran J Vet Res 12, 127132.
55. Stone, DAJ, Allan, GL & Anderson, AJ (2003) Carbohydrate utilization by juvenile silver perch, Bidyanus bidyanus (Mitchell). IV. Can dietary enzymes increase digestible energy from wheat starch, wheat and dehulled lupin? Aquacult Res 34, 135147.
56. Mahmoud, MMA, Kilany, OE & Dessouki, AA (2014) Effects of fish meal replacement with soybean meal and use of exogenous enzymes in diets of Nile tilapia (Oreochromisniloticus) on growth, feed utilization, histopathological changes and blood parameters. Life Sci J 11, 618.


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