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Dietary fat affects lipids and anti-cardiolipin antibody levels in autoimmune-prone NZB/W F1 mice

  • Bi-Fong Lin (a1), Su-Jen Jeng (a1), Bor-Luen Chiang (a2) and Chao-Chi Huang (a1)

Abstract

Studies in autoimmune-prone NZB/W F1 mice have demonstrated that the amount of dietary fat can affect autoantibody production and the disease course of autoimmune diseases. Anti-cardiolipin antibodies have been found to play a major role in thrombus formation and the increase of abortion rate in both human lupus patients and murine lupus. The present study investigated further the effect of dietary fat on lipid and anti-cardiolipin antibody production in autoimmune-prone mice. Two groups of NZB/W F1 mice were fed on diets containing 200 g dietary fat/kg and 50 g dietary fat/kg respectively, the fat being composed of equal amounts of lard and soyabean oil. Serum levels of lipids, immunoglobulin (Ig) anti-single stranded DNA and anti-cardiolipin antibodies were followed regularly every month and mice were killed for in vitro experiments after 5 months on the experimental diets. The results showed that serum triacylglycerol concentration was lower in mice fed on the high-fat diet than in those fed on 50 g fat/kg. There was no significant difference in hepatic lipid contents; however, the fatty acid contents were different between these two groups. Hepatic linoleic acid (18:2n−6) and arachidonic acid (20:4n−6) concentrations were higher in mice fed on the high-fat diet. There were no significant differences in serum IgM concentrations or IgM anti-cardiolipin antibody levels between these two groups. However, IgG anti-cardiolipin antibody levels were higher in mice fed on the high-fat diet at the age of 3–4 months. Total serum IgG concentration was noted to be higher, but in contrast, serum IgA was lower, in the high-dietary-fat group. These findings suggest that high dietary fat may affect lipid metabolism and autoantibody levels in autoimmune diseases

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References

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Berger, A., Gershwin, M. E. & German, J. B. (1992). Effects of various dietary fats on cardiolipin acyl composition during ontogeny of mice. Lipids 27, 605612.
Carlson, S. E. & Goldfarb, S. (1979). A sensitive enzymatic method for the determination of free and esterified tissue cholesterol. Clinica Chimica Acta 79, 575582.
Carroll, P., Stafford, D., Schwartz, R. S. & Stollar, B. D. (1985). Murine monoclonal anti-DNA autoantibodies bind to endogenous bacteria. Journal of Immunology 135, 10861090.
Carteron, N. L., Schimenti, C. L. & Wofsy, D. (1989). Treatment of murine lupus with F(ab')2 fragments of monoclonal antibody to L3T4. Suppression of autoimmunity does not depend on T helper cell depletion. Journal of Immunology 142, 14701475.
Datta, S. K., Patel, H. & Berry, D. (1987). Induction of cationic shift in IgG anti-DNA autoantibodies. Role of T helper cells with classical and novel phenotypes in three murine models of lupus nephritis. Journal of Experimental Medicine 165, 12521268.
Eilat, D., Zlomick, A. Y. & Fischel, R. (1986). Evaluation of the cross-reaction between anti-DNA and anticardiolipin antibodies in SLE and experimental animals. Clinical Experimental Immunology 65, 269278.
Fernandes, G., Yunis, E. J. & Good, R. A. (1976). Influence of diet on survival of mice. Proceedings of the National Academy of Sciences USA 73, 12791283.
Folch, J., Lees, M. & Sloane-Stanley, G. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497509.
Fossati, P. & Prencipe, L. (1982). Serum triglycerides determined colonmetrically with an enzyme that produces hydrogen peroxide. Clinical Chemistry 28, 20772080.
Gajjar, A., Kubo, C., Johnson, B. C. & Good, R. A. (1987). Influence of extremes of protein and energy intake on survival of B/W mice. Journal of Nutrition 117, 11361140.
Haeeis, E. N., Gharavi, A. E., Patel, S. P. & Hughes, G. R. V. (1987). Evaluation of the anticardiolipin antibody test: report of an international workshop held April 1986. Clinical Experimental Immunology 68, 212215.
Harris, E. N., Gharavi, A. E., Boey, M. L., Patel, B. M., Mackworth-Young, C. G., Loizou, S. & Hughes, G. R. V. (1983). Anticardiolipin antibodies: detection by radioimmunoassay and association with thrombosis in systemic lupus erythematosus. Lancet 2, 12111214.
Hashimoto, Y., Kawamura, M., Ichikawa, K., Suzuki, T., Sumida, T., Yoshida, S., Matsuura, E., Ikehara, S. & Koike, T. (1992). Anticardiolipin antibodies in NEW x BXSB F1 mice: a model of antiphospholipid syndrome. Journal of Immunology 149, 10631068.
Hurd, E. T., Johnston, J. M, Okita, J. R., MacDonald, P. C., Ziff, M. & Gilliam, J. N. (1981). Prevention of glomerulonephritis and prolonged survival in New Zealand Black/New Zealand White F1 hybrid mice fed on essential fatty acid-deficient diet. Journal of Clinical Investigation 67, 476483.
Koike, T., Sueishi, M., Funaki, H., Tomioka, H. & Yoshida, S. (1984). Antiphospholipid antibodies and biological false positive serological test for syphilis in patients with systemic lupus erythematosus. Clinical Experimental Immunology 56, 193199.
Koike, T., Tomioka, H. & Kumagai, A. (1982). Antibodies cross-reactive with DNA and cardiolipin in patients with systemic lupus erythematosus. Clinical Experimental Immunology 50, 298.
Kubo, C., Johnson, B. C., Day, N. K. & Good, R. A. (1984). Calorie source, calorie restriction, immunity and aging of (NZB/NEW) F1 mice. Journal of Nutrition 114, 18841899.
Lafer, E. M., Raugh, J., Andrzejewski, C. Jr, Mudd, D., Furie, B., Schwarz, R. S. & Stollar, B. D. (1981). Polyspecific monoclonal lupus autoantibodies reactive with both polynuclmtides and phospholipids. Journal of Experimental Medicine 153, 897909.
Lee, M.-H., Wang, M.-L. & Min, B.-W. (1990). Effects of methylation on determination of fatty acids. Food Science 17, 110.
Levy, J. A., Ibrahim, A. B., Shirai, T., Ohta, K., Nagasawa, R., Yoshida, H., Estes, J. & Gardner, M. (1982). Dietary fat affects immune response, production of antiviral factors, and immune complex disease in NZB/NZW mice. Proceedings of the Nationul Academy of Sciences USA 79, 19741978.
Lin, B.-F., Huang, C.-C., Chiang, B.-L. & Jeng, S. -J. (1996). Dietary fat influences Ia antigen expression, cytokines and prostaglandin E2 production of immune cells in autoimmune-prone NZB x NZW F1 mice. British Journal of Nutrition 75, 711722.
Lockshin, M. D., Druzin, M. L., Goei, S., Qamar, T., Magid, M. S., Janovic, L. & Ferenc, M. (1985). Antibody to cardiolipin as a predictor of fetal distress or death in pregnant patients with systemic lupus erythematosus. New England Journal of Medicine 313, 152156.
MacGregor, A. J., Dhillon, V. B., Binder, A., Forte, C. A., Knight, B. C., Betterridge, D. J. & Isenberg, D. A. (1992). Fasting lipids and anticardiolipin antibodies as risk factors for vascular disease in systemic lupus erythematosus. Annals of Rheumatic Diseases 51, 152155.
Mackworth-Young, C. (1990). Antiphospholipid antibodies: more than just a disease marker? Immunology Today 11, 6065.
McNeil, H. P., Chesterman, C. N. & Krills, S. A. (1991). Immunology and clinical importance of antiphospholipid antibodies. Advances in Immunology 49, 193280.
Morrow, W. J. W., Ohashi, Y., Hall, J., Pribnow, J., Hirose, S., Shirai, T. & Levy, J. A. (1985). Dietary fat and immune function. I. Antibody response, lymphocyte and accessory cell function in (NZB x NZW) F1 mice. Journal of Immunology 135, 38573863.
National Research Council (1985). Guide for the Care and Use of Laboratory Animals. Publication no. 85–23 (revised). Bethesda, MD: National Institutes of Health.
Prickett, J. D., Robinson, D. R. & Steinberg, A. D. (1981). Dietary enrichment with the polyunsaturated fatty acid eicosapentaenoic acid prevents proteinuria and prolongs survival in NZB/W F1 mice. Journal of Clinical Investigation 68, 556559.
Rauch, J., Tannenbaum, H., Stollar, B. D. & Schwartz, S. (1984). Monoclonal anti-cardiolipin antibodies bind to DNA. European Journal of Immunology 14, 529534.
Richmond, N. (1973). Preparation and properties of a bacterial cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clinical Chemistry 19, 13501356.
Rupin, A., Gruel, Y., Watier, H., Girard, A. C., Leroy, J. & Bardos, P. (1991). ELISA for the detection of anticardiolipin antibodies. High specificity based on the use of adult bovine serum as buffer and systematic subtraction of non-specific binding. Journal of Immunological Methods 138, 225231.
Santoli, D. & Zurier, R. B. (1989). Prostaglandin E precursor fatty acids inhibit human IL-2 production by a prostaglandin E-independent mechanism. Journal of Immunology 143, 13031309.
Shoenfeld, Y., Rauch, J., Massicotte, H., Datta, S. K., Andre-Schwartz, J., Stollar, B. D. & Schwartz, R. S. (1983). Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. New England Journal of Medicine 308, 414420.
Snyder, D. S., Beller, D. I. & Unanue, E. R. (1982). Prostaglandins modulate macrophage Ia expression. Nature 299, 163165.
Steinberg, A. D., Huston, D. P., Taurog, J. D., Cowdery, J. S. & Raveche, E. S. (1981). The cellular and genetic basis of murine lupus. Immunology Review 55, 121154.
Takeyama, M., Itoh, S., Nayasaki, T. & Tanimazu, I. (1977). A new enzymatic method for determinant of serum choline-containing phospholipids. Clinica Chimica Acta 79, 9398.
Theofilopoulos, A. N. & Dixon, F. J. (1981). Etiopathogenesis of murine SLE. Immunology Review 55, 179216.
Theofilopoulos, A. N., Kofler, R., Singer, P. A. & Dixon, F. J. (1989). Molecular genetics of murine lupus models. Advances in Immunology 46, 61109.

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