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Detection of caseinophosphopeptides in the distal ileostomy fluid of human subjects

Published online by Cambridge University Press:  09 March 2007

H. Meisel ,
H. Meisel ,
S. Fairweather-Tait ,
R. J. FitzGerald ,
R. Hartmann ,
C. N. Lane ,
D. McDonagh ,
B. Teucher  and
J. M. Wal
H. Meisel*
Affiliation:
Federal Dairy Research Centre, Institute for Dairy Chemistry and Technology, Kiel, Germany
H. Meisel*
Affiliation:
Laboratoire d'Immuno-Allergie Alimentaire, INRA-CEA, SPI, Gif sur Yvette, France
S. Fairweather-Tait
Affiliation:
Institute of Food Research, Norwich Research Park, Norfolk, UK
R. J. FitzGerald
Affiliation:
University of Limerick, Life Sciences Department, Limerick, Republic of Ireland
R. Hartmann
Affiliation:
Federal Dairy Research Centre, Institute for Dairy Chemistry and Technology, Kiel, Germany
C. N. Lane
Affiliation:
Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Republic of Ireland
D. McDonagh
Affiliation:
Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Republic of Ireland
B. Teucher
Affiliation:
Institute of Food Research, Norwich Research Park, Norfolk, UK
J. M. Wal
Affiliation:
Laboratoire d'Immuno-Allergie Alimentaire, INRA-CEA, SPI, Gif sur Yvette, France
*
*Corresponding author: Professor Hans Meisel, fax +49 431 609 2300, email meisel@bafm.de
*Corresponding author: Professor Hans Meisel, fax +49 431 609 2300, email meisel@bafm.de
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Abstract

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Caseinophosphopeptides (CPP) were detected for the first time in ileostomy fluid, collected at 2 h intervals for 10 h post milk and CPP ingestion, from human volunteers with an ileostomy. The level of CPP present in ileostomy fluid obtained from milk-fed volunteers was markedly higher than that from volunteers fed with selected CPP preparations. The findings are based on HPLC analysis in combination with peptide-bound P determination, thin-layer electrophoresis and amino acid analysis, together with ELISA studies using polyclonal antibodies raised against a set of CPP to detect immunoreactive CPP in ileostomy fluid. These procedures allowed the detection of nm concentrations of CPP. CPP, which can be released during intestinal digestion, may function as bioactive constituents and carriers for different minerals, especially Ca, and may be used as ingredients in functional foods or pharmaceutical preparations.

Keywords

CaseinophosphopeptidesMilkHuman ileostomy samplesBioactive peptides
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 3 , March 2003 , pp. 351 - 358
Copyright
Copyright © The Nutrition Society 2003

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