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Day-to-day variation in iron-status measures in young iron-deplete women

Published online by Cambridge University Press:  08 March 2007

Anita Belza ,
Marianne Henriksen ,
Annette K Ersbøll ,
Shakuntala H Thilsted  and
Inge Tetens
Anita Belza*
Affiliation:
Department of Human Nutrition, Centre for Advanced Food Studies, Royal Veterinary and Agricultural University, Denmark
Marianne Henriksen
Affiliation:
Department of Human Nutrition, Centre for Advanced Food Studies, Royal Veterinary and Agricultural University, Denmark Nycomed A/S, Roskilde, Denmark
Annette K Ersbøll
Affiliation:
Department of Large Animal Sciences, Royal Veterinary and Agricultural University, Denmark
Shakuntala H Thilsted
Affiliation:
Department of Human Nutrition, Centre for Advanced Food Studies, Royal Veterinary and Agricultural University, Denmark
Inge Tetens
Affiliation:
Department of Human Nutrition, Centre for Advanced Food Studies, Royal Veterinary and Agricultural University, Denmark
*
*Corresponding author: Anita Belza, fax +45 35282483, email anbe@kvl.dk
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Abstract

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In intervention and observational studies, it is necessary to determine the number of blood samples required to estimate the true value of Fe-status measures. The aim of the present study was to determine the number of days for blood sampling required in order to measure the ‘true value’ of five Fe-status parameters in young Fe-depleted women and to investigate the effect of menstrual cycle on these measures. Twelve women (aged 23–30 years), non-anaemic but with low Fe stores, participated in the study. Venous blood samples were collected under standardised conditions on fifteen non-consecutive days during a 5-week period. All blood samples were analysed for Hb, serum ferritin (SF), serum transferrin receptors (sTfR), red blood cell volume distribution width (RDW) and reticulocytes (RET), and body Fe stores were calculated as the ratio between sTfR and SF. No systematic changes were found in the investigated parameters during the study. When analytical variations were accounted for, the day-to-day variations (CV%) were as follows: Hb 2·9 %, SF 8·2 %, RET 26·0 %, RDW 2·4 % and sTfR 8·1 %. Calculating the ‘true value’ with a 5 % significance level and 80 % power showed that one blood sample was sufficient for Hb, SF, sTfR and RDW, whereas seven blood-sampling days were needed for RET. In this study, no significant differences in Fe status were found across the menstrual cycle. The conclusions from this study are valid for studies conducted under similar strict conditions.

Keywords

Iron-status measuresDay-to-day variabilityMenstrual cycle
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 4 , October 2005 , pp. 551 - 556
Copyright
Copyright © The Nutrition Society 2005

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