Skip to main content Accessibility help
×
Home

Comparative effects of fructose and glucose on the lipid and carbohydrate metabolism of perfused rat liver

  • D. L. Topping (a1) and P. A. Mayes (a1)

Abstract

1. Livers from rats fed on a standard diet were perfused with whole blood, and infused continuously with glucose and fructose at equimolar rates.

2. Infusion of fructose increased both the secretion of very-low-density-lipoprotein (VLDL)-triglycerides and the incorporation of free fatty acids (FFA) from the perfusate into VLDL-lipids, but neither of these two processes was affected by infusion of glucose.

3. Infusion of fructose decreased the oxidation and increased the esterification of FFA, but glucose infusion had no effect on these processes. When fructose and glucose were infused together there was a further decrease in oxidation.

4. When fructose was infused alone or together with glucose, blood concentrations rapidly became stabilized at those found in the hepatic portal vein in vivo, with similar rates of hepatic uptake to those found in the intact animal. Infusion of glucose alone resulted in continuously increasing perfusate glucose concentrations, and rates of uptake which were only 20 % of those for fructose. Blood glucose concentrations were reduced, and lactate concentrations were increased by fructose infusion, and when glucose and fructose were infused together there was a greatly increased rate of glucose uptake.

5. Liver glycogen was not affected by the infusion of fructose or glucose alone; however, their combined addition significantly increased its concentration.

6. Uptake of perfusate FFA was not affected by either fructose or glucose infusions.

7. The results are discussed in terms of the differences in nutrition and metabolism between glucose and fructose, with particular reference to the development of hyper- triglyceridaemia.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Comparative effects of fructose and glucose on the lipid and carbohydrate metabolism of perfused rat liver
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Comparative effects of fructose and glucose on the lipid and carbohydrate metabolism of perfused rat liver
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Comparative effects of fructose and glucose on the lipid and carbohydrate metabolism of perfused rat liver
      Available formats
      ×

Copyright

References

Hide All
Bremer, J., Bjerre, M. S., Christophersen, B. O., Daae, L. N. W., Solberg, H. E. & Aas, M. (1974). In Regulation of Hepatic Metabolism, p. 159 [Lundquist, F. and Tygstrup, N., editors]. Copenhagen: Einar Munksgaard.
Brownlee, K. A. (1949). Industrial Experimentation, 4th ed. London: H.M. Stationery Office.
Bruce, H. M. & Parkes, A. S. (1949). J. Hyg., Cantb. 47, 202. Carlson, L. A. (1959). Acta Soc. Med. upsal. 64, 208.
Chernick, S. S., Chaikoff, I. L. & Abraham, S. (1951). J. biol. Chem. 193, 793.
Denton, R. M. & Randle, P. J. (1967). Biochem. J. 104, 423.
Felts, J. M. & Mayes, P. A. (1967). Biochem. J. 105, 735.
Fritz, I. B. (1961). Physiol. Rev. 41, 52.
Good, C. A., Kramer, H. & Somogyi, M. (1933). J. biol. Chem. 100, 485.
Havel, R. J., Felts, J. M. & Van Duyne, C. M. (1962). J. Lipid Res. 3, 297.
Heinz, F. & Junghänel, J. (1969). Hoppe-Seyler's Z. physiol. Chm. 350, 859.
Hers, H. G., Stalmans, W., de Wulf, H., Laloux, M. & Hue, L. (1974). In Regulation of Hepatic Metabolism, p. 237 [Lundquist, F. and Tygstrup, N., editors]. Copenhagen: Einar Munksgaard.
Hohorst, H. J. (1965). In Methods of Enzymutic Analysis, p. 266 [Bergmeyer, H. U. editor]. London and New York: Academic Press.
Krebs, H. A. & Henseleit, K. (1932). Hoppe-Seyler's Z. plzysiol, Chem. 210, 33.
Long, E. C. (1957). Studies in foetal carbohydrate metabolism. PhD Thesis, University of London.
Macdonald, I. (1965). Clin. Sci. 29, 193.
Mayes, P. A. & Felts, J. M. (1966). Proc. Eur. Soc. Stud. Drug Toxic. 7, 16.
Mayes, P. A. & Felts, J. M. (1967). Nature, Lond. 215, 716.
Mayes, P. A. & Topping, D. L. (1974). Biochem. J. 140, 111.
Naismith, D. J. (1971). Proc. Nutr. Soc. 30, 259.
Pereira, J. N. & Jangaard, N. O. (1971). Metabolism 20, 392.
Phillips, M. J., Hetenyi, A. & Adachi, F. (1970). Lab. Inwest. 22, 370.
Renold, A. E., Hastings, A. B. & Nesbett, F. B. (1954). J. biol. Chem. 29, 687.
Roe, J. H. (1934) .J. biol. Chem. 107, 15.
Seglen, P. O. (1974). Biochim. biophys. Acta 338, 317.
Sillero, M. A. G., Sillero, A. & Sols, A. (1969). Eur. J. Biochem. 10, 345.
Stein, Y. & Shapiro, B. (1959). Am. J. Physiol. 196, 1238.
Topping, D. L. & Mayes, P. A. (1971). Nutr. Metab. 13, 331.
Topping, D. L. & Mayes, P. A. (1972). Biochem. J. 126, 295.
Topping, D. L. & Mayes, P. A. (1973). Biochem. Soc. Trans. 1, 433.
Trout, D. L., Estes, E. H. & Friedberg, S. J. (1960). J. Lipid Res. 1, 199.
Tzur, R., Tal, E. & Shapiro, B. (1964). Biochim. biophys. Acta 84, 18.
Underwood, A. H. & Newsholme, E. A. (1965). Biochem. J. 95, 767.
Walli, A. K., Birkmann, L. & Schimassek, H. (1976). Biochem. Sac. Trans. (In the Press.)
Wieland, O. H., Siess, E. A., Weiss, L., Löffler, A., Patzelt, C., Portenhauser, R., Hartmann, V. & Schirmann, S. (1973). Symp. Sac. exp. Biol. 23, 371.
Windmueller, H. G. & Spaeth, A. E. (1967). Archs Biochem. Biophys. 122, 362.
Woods, H. F., Eggleston, L. V. & Krebs, H. A. (1970). Biochem. J. 119, 501.

Comparative effects of fructose and glucose on the lipid and carbohydrate metabolism of perfused rat liver

  • D. L. Topping (a1) and P. A. Mayes (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed