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Cocoa powder enhances the level of antioxidative activity in rat plasma

Published online by Cambridge University Press:  09 March 2007

Seigo Baba ,
Naomi Osakabe ,
Midori Natsume ,
Akiko Yasuda ,
Toshio Takizawa ,
Tetsuo Nakamura  and
Junji Terao
Seigo Baba*
Affiliation:
Functional Foods R&D Laboratories, Meiji Seika Kaisha Ltd, 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan
Naomi Osakabe
Affiliation:
Functional Foods R&D Laboratories, Meiji Seika Kaisha Ltd, 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan
Midori Natsume
Affiliation:
Functional Foods R&D Laboratories, Meiji Seika Kaisha Ltd, 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan
Akiko Yasuda
Affiliation:
Functional Foods R&D Laboratories, Meiji Seika Kaisha Ltd, 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan
Toshio Takizawa
Affiliation:
Functional Foods R&D Laboratories, Meiji Seika Kaisha Ltd, 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan
Tetsuo Nakamura
Affiliation:
Functional Foods R&D Laboratories, Meiji Seika Kaisha Ltd, 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan
Junji Terao
Affiliation:
Department of Nutrition, School of Medicine, The University of Tokushima, Tokushima, Japan
*
*Corresponding author: Seigo Baba, fax +81 492 84 7569, email seigo_baba@meiji.co.jp
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Abstract

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The aims of the present study were to determine the level of (-)-epicatechin (EC) and its metabolites in rat plasma after oral administration of cocoa powder and to evaluate the protective effect of cocoa powder in terms of suppressing the oxidation of plasma components. Rats were orally administered 1 g cocoa powder/kg body weight, containing 7·80 mg EC, and their blood was collected before administration and at designated time intervals thereafter. The EC and its metabolites in plasma were treated with β-glucuronidase and/or sulfatase, then analysed by HPLC and by liquid chromatography–MS. Several EC-related compounds were detected in plasma such as free EC, and glucuronide, sulfate, and glucuronide–sulfate conjugates of non-methylated or methylated EC. All EC metabolites showed a maximum concentration in plasma at 30–60 min post-administration. Glucuronide conjugates of both non-methylated and methylated EC were found in high concentration in plasma. Moreover, administration of cocoa powder significantly reduced the accumulation of lipid peroxides in plasma and significantly reduced the consumption of α-tocopherol in plasma oxidized by treatment with 2,2′-azobis-(2-amidinopropane) dihydrochloride (AAPH (25 mmol/l)) or CuSO4 (100 μmol/l) compared with that in the case of plasma obtained before administration. The total EC concentration in plasma was negatively correlated with the level of accumulation of lipid peroxides in plasma oxidized by treatment with AAPH (25 mmol/l) and was positively correlated with the level of residual α-tocopherol in plasma oxidized by treatment with CuSO4 (100 μmol/l). These results indicate that EC in cocoa powder was absorbed from the digestive tract, that various conjugated forms of EC were generated in the digestive tract and distributed to the plasma, and that these enhanced the antioxidative activity of plasma.

Keywords

Cocoa powderEpicatechinAbsorptionAntioxidant
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 5 , November 2000 , pp. 673 - 680
Copyright
Copyright © The Nutrition Society 2000

References

Afanas'ev, IB, Dorozhko, AI, Brodskii, AV, Kostyuk, VA and Potapovitch, AI (1989) Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation.Biochemical Pharmacology 38, 1763–1769.CrossRefGoogle ScholarPubMed
Bors, W, Heller, W, Michel, C and Sarah, M (1990) Flavonoids as antioxidants: Determination of radical-scavenging efficiencies.Methods in Enzymology 186, 343–355.CrossRefGoogle ScholarPubMed
Cook, NC and Samman, S (1996) Flavonoids: chemistry, metabolism, cardioprotective effects, and dietary sources.Journal of Nutritional Biochemistry 7, 66–76.CrossRefGoogle Scholar
Hammerstone, JF, Lazarus, SA, Mitchell, AE, Rucker, R and Schmitz, HH (1999) Identification of procyanidins in cocoa Theobroma cacao and chocolate using high-performance liquid chromatography/mass spectrometry.Journal of Agricultural and Food Chemistry 47, 490–496.CrossRefGoogle ScholarPubMed
Harada, M, Kan, Y, Naoki, H, Fukui, Y, Kageyama, N, Nakai, M, Miki, W and Kiso, Y (1999) Identification of the major antioxidative metabolites in biological fluids of the rat with ingested (+)-catechin and (-)-epicatechin.Bioscience, Biotechnology, and Biochemistry 63, 973–977.CrossRefGoogle ScholarPubMed
Hertog, MGL, Feskens, EJM, Hollman, PCH, Katan, MB and Kromhout, D (1993) Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.Lancet 342, 1007–1011.CrossRefGoogle ScholarPubMed
Hertog, MGL, Kromhout, D, Aravanis, C, Blackburn, H, Buzina, R, Fidanza, F, Giampaoli, S, Jansen, A, Menotti, A, Nedeljkovic, S, Pekkarinen, M, Simic, BS, Toshima, H, Feskens, EJM, Hollman, PCH and Katan, MB (1995) Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study.Archives of Internal Medicine 155, 381–386.CrossRefGoogle ScholarPubMed
Hollman, PCH, Tijburg, LBM and Yang, CS (1997) Bioavailability of flavonoids from tea.Critical Reviews in Food Science and Nutrition 37, 719–738.CrossRefGoogle ScholarPubMed
Ishikawa, T, Suzukawa, M, Ito, T, Yoshida, H, Ayaori, M, Nishiwaki, M, Yonemura, A, Hara, Y and Nakamura, H (1997) Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification.American Journal of Clinical Nutrition 66, 261–266.CrossRefGoogle ScholarPubMed
Jankun, J, Selman, SH, Swiercz, R and Skrzypczak-Jankun, E (1997) Why drinking green tea could prevent cancer.Nature 387, 561.CrossRefGoogle ScholarPubMed
Keli, SO, Hertog, MGL, Feskens, EJM and Kromhout, D (1996) Dietary flavonoids, antioxidant vitamins, and incidence of stroke. The Zutphen study.Archives of Internal Medicine 154, 637–642.CrossRefGoogle Scholar
Knekt, P, Järvinen R, Reunanen, A and Maatela, J (1996) Flavonoid intake and coronary mortality in Finland: a cohort study.British Medical Journal 312, 478–481.CrossRefGoogle ScholarPubMed
Lee, M, Wang, Z, Li, H, Chen, L, Sun, Y, Gobbo, S, Balentine, DA and Yang, CS (1995) Analysis of plasma and urinary tea polyphenol in human subjects.Cancer Epidemiology, Biomarkers and Prevention 4, 393–399.Google ScholarPubMed
Lotito, SB and Fraga, CG (1998) (+)-Catechin prevents human plasma oxidation.Free Radical Biology and Medicine 24, 435–441.CrossRefGoogle ScholarPubMed
Nanjo, F, Goto, K, Seto, R, Suzuki, M, Sakai, M and Hara, Y (1996) Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical.Free Radical Biology and Medicine 21, 895–902.CrossRefGoogle Scholar
Oguni, I, Cheng, SJ, Lin, PZ and Hara, Y (1992) Protection against cancer risk by Japanese green tea.Preventive Medicine 21, 332.Google Scholar
Okushio, K, Suzuki, M, Matsumoto, N, Nanjo, F and Hara, Y (1999) Identification of (-)-epicatechin metabolites and their metabolic fate in the rat.Drug Metabolism and Disposition 27, 309–316.Google ScholarPubMed
Osakabe, N, Sanbongi, C, Yamagishi, M, Takizawa, T and Osawa, T (1998) Effects of polyphenol substances derived from. Theobroma cacao on gastric mucosal lesion induced by ethanol. Bioscience, Biotechnology, and Biochemistry 62, 1535–1538.CrossRefGoogle ScholarPubMed
Osakabe, N, Yamagishi, M, Sanbongi, C, Natsume, M, Takizawa, T and Osawa, T (1998) The antioxidative substances in cacao liquor.Journal of Nutritional Science and Vitaminology 44, 313–321.CrossRefGoogle ScholarPubMed
Piskula, MK and Terao, J (1998) Accumulation of (-)-epicatechin metabolites in rat plasma after oral administration and distribution of conjugation enzymes in rat tissues.Journal of Nutrition 128, 1172–1178.CrossRefGoogle ScholarPubMed
Price, MP and Butler, LG (1977) Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain.Journal of Agricultural and Food Chemistry 25, 1268–1273.CrossRefGoogle Scholar
Ratty, AK and Das, NP (1988) Effects of flavonoids on nonenzymatic lipid peroxidation: Structure–activity relationships.Biochemical Medicine and Metabolic Biology 39, 69–79.CrossRefGoogle Scholar
Rice-Evans, CA, Miller, NJ and Paganga, G (1996) Structure–antioxidant activity relationships of flavonoids and phenolic acids.Free Radical Biology and Medicine 20, 933–956.Google ScholarPubMed
Richelle, M, Tavazzi, I, Enslen, M and Offord, EA (1999) Plasma kinetics in man of epicatechin from black chocolate.European Journal of Clinical Nutrition 53, 22–26.CrossRefGoogle ScholarPubMed
Sanbongi, C, Osakabe, N, Natsume, M, Takizawa, T, Gomi, S and Osawa, T (1998) Antioxidative polyphenols isolated from.Theobroma cacao. Journal of Agricultural and Food Chemistry 46, 454–457.CrossRefGoogle ScholarPubMed
Silva, EL, Piskula, M and Terao, J (1998) Enhancement of antioxidative ability of rat plasma by oral administration of (-)-epicatechin.Free Radical Biology and Medicine 24, 1209–1216.CrossRefGoogle ScholarPubMed
Silva, JMR, Rigaud, J, Cheynier, V, Cheminat, A and Moutounet, M (1991) Procyanidin dimers and trimers from grape seeds.Phytochemistry 30, 1259–1264.CrossRefGoogle Scholar
Terao, J, Piskula, M and Yao, Q (1994) Protective effect of epicatechin, epicatechin gallate, and quercetin on lipid peroxidation in phospholipid bilayers.Archives of Biochemistry and Biophysics 308, 278–284.CrossRefGoogle ScholarPubMed
Thompson, RS, Jacques, D, Haslam, E and Tanner, RJN (1972) Plant proanthocyanidins. Part I. Introduction; the isolation, structure, and distribution in nature of plant procyanidins.Journal of the Chemical Society Perkin Transactions 1 13, 1387–1399.CrossRefGoogle Scholar
Yamanaka, N, Oda, O and Nagao, S (1997) Green tea catechins such as (-)-epicatechin and (-)-epigallocatechin accelerate Cu2+-induced low density lipoprotein oxidation in propagation phase.FEBS Letters 401, 230–234.CrossRefGoogle ScholarPubMed
Yang, CS, Chen, L, Lee, MJ, Balentine, D, Kuo, MC and Schantz, SP (1998) Blood and urine levels of tea catechins after ingestion of different amounts of green tea by human volunteers.Cancer Epidemiology, Biomarkers and Prevention 7, 351–354.Google ScholarPubMed
Yoshino, K, Tomita, I, Sano, M, Oguni, I, Hara, Y and Nakano, M (1994) Effects of long-term dietary supplement of tea polyphenols on lipid peroxide levels in rats.Age 17, 79–85.CrossRefGoogle Scholar
Yu, G and Hsieh, C (1991) Risk factors for stomach cancer: a population-based case–control study in Shanghai.Cancer Causes and Control 2, 169–174.CrossRefGoogle ScholarPubMed
van Acker, SA, van den Berg, DJ, Tromp, MN, Griffioen, DH, van Bennekom, WP, van der Vijgh, WJ and Bast, A (1996) Structural aspects of antioxidant activity of flavonoids.Free Radical Biology and Medicine 20, 331–342.CrossRefGoogle ScholarPubMed
Zhang, A, Zhu, QY, Luk, YS, Ho, KY, Fung, KP and Chen, Z (1997) Inhibitory effects of jasmine green tea epicatechin isomers on free radical-induced lysis of red blood cells.Life Sciences 61, 383–394.CrossRefGoogle Scholar