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Changes in food intake, metabolic parameters and insulin resistance are induced by an isoenergetic, medium-chain fatty acid diet and are associated with modifications in insulin signalling in isolated rat pancreatic islets

  • A. C. Marçal (a1), J. P. G. Camporez (a1), T. M. Lima-Salgado (a1), D. E. Cintra (a2), E. H. Akamine (a1), L. M. Ribeiro (a1), F. N. Almeida (a1), R. P. Zanuto (a1), R. Curi (a1), S. C. Boldrini (a3), E. A. Liberti (a3), J. Fiamoncini (a1), S. M. Hirabara (a4), F. C. Deschamps (a5), A. R. Carpinelli (a1) and C. R. O. Carvalho (a1)...

Abstract

Long-chain fatty acids are capable of inducing alterations in the homoeostasis of glucose-stimulated insulin secretion (GSIS), but the effect of medium-chain fatty acids (MCFA) is poorly elucidated. In the present study, we fed a normoenergetic MCFA diet to male rats from the age of 1 month to the age of 4 months in order to analyse the effect of MCFA on body growth, insulin sensitivity and GSIS. The 45 % MCFA substitution of whole fatty acids in the normoenergetic diet impaired whole body growth and resulted in increased body adiposity and hyperinsulinaemia, and reduced insulin-mediated glucose uptake in skeletal muscle. In addition, the isolated pancreatic islets from the MCFA-fed rats showed impaired GSIS and reduced protein kinase Bα (AKT1) protein expression and extracellular signal-related kinase isoforms 1 and 2 (ERK1/2) phosphorylation, which were accompanied by increased cellular death. Furthermore, there was a mildly increased cholinergic sensitivity to GSIS. We discuss these findings in further detail, and advocate that they might have a role in the mechanistic pathway leading to the compensatory hyperinsulinaemic status found in this animal model.

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Corresponding author

*Corresponding author: Professor A. C. Marçal, fax +55 79 21056622, email acmarcal@yahoo.com.br

References

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Keywords

Changes in food intake, metabolic parameters and insulin resistance are induced by an isoenergetic, medium-chain fatty acid diet and are associated with modifications in insulin signalling in isolated rat pancreatic islets

  • A. C. Marçal (a1), J. P. G. Camporez (a1), T. M. Lima-Salgado (a1), D. E. Cintra (a2), E. H. Akamine (a1), L. M. Ribeiro (a1), F. N. Almeida (a1), R. P. Zanuto (a1), R. Curi (a1), S. C. Boldrini (a3), E. A. Liberti (a3), J. Fiamoncini (a1), S. M. Hirabara (a4), F. C. Deschamps (a5), A. R. Carpinelli (a1) and C. R. O. Carvalho (a1)...

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