Skip to main content Accessibility help
×
Home

Bone-protective effects of bioactive fractions and ingredients in Sambucus williamsii HANCE

  • Hui-Hui Xiao (a1), Yi Dai (a2), Hoi-Ying Wan (a3), Man-Sau Wong (a3) (a4) and Xin-Sheng Yao (a1) (a2)...

Abstract

Our previous study demonstrated that 60 % ethanol crude extract of Sambucus williamsii HANCE (SWH) improved bone mass, bone strength and bone micro-structure in both ovariectomised (OVX) rats and mice. The present study aims to identify the bioactive fractions and ingredients in SWH that account for its osteoprotective effects. Bilateral sham-operated mice acted as controls. OVX C57BL/6J mice, aged 12 weeks, were orally administrated daily with vehicle or 17β-oestradiol (3·2 mg/kg), SWH (60 % ethanol crude extract; 1·0 g/kg), SWA (water eluate; 0·570 g/kg), SWB (30 % ethanol eluate; 0·128 g/kg) or SWC (50 and 95 % ethanol eluates; 0·189 g/kg) for 12 weeks. The effects of the different fractions on bone properties in the OVX mice model were studied. In addition, their effects on osteoblast proliferation and differentiation were evaluated in UMR 106 cells. SWC significantly restored bone mineral density and improved bone size and bone content parameters in the femur and tibia as well as increased biomechanical strength at the tibia diaphysis in OVX mice. Similarly, SWC was the most potent fraction in stimulating cell proliferation and differentiation in UMR 106 cells. Also, SWC did not alter uterus weight in OVX mice. Nine major peaks, seven lignans and two phenolic acids, in the HPLC fingerprint of the SWC fraction were identified, isolated and characterised. In conclusion, the present study demonstrated that SWC was the most potent fraction in SWH that exerted anti-osteoporotic effects. Furthermore, lignans might be the potential bioactive components in SWC.

Copyright

Corresponding author

*Corresponding authors: Dr Man-Sau Wong, fax +852 23649932, email bcmswong@polyu.edu.hk; Professor Xin-Sheng Yao, fax +86 20 85225849, email yaoxinsheng@vip.tom.com

References

Hide All
1 Lock, CA, Lecouturier, J, Mason, JM, et al. (2006) Lifestyle interventions to prevent osteoporotic fractures: a systematic review. Osteoporos Int 17, 2028.
2 Notelovitz, M (1997) Estrogen therapy and osteoporosis: principles and practice. Am J Med Sci 313, 212.
3 Davison, S & Davis, SR (2003) Hormone replacement therapy: current controversies. Clin Endocrinol 58, 249261.
4 Beral, V, Banks, E, Reeves, G, et al. (1999) Use of HRT and the subsequent risk of cancer. J Epidemiol Biostat 4, 191210.
5 Persson, I, Weiderpass, E, Bergkvist, L, et al. (1999) Risks of breast and endometrial cancer after estrogen and estrogen–progestin replacement. Cancer Causes Control 10, 253260.
6 Rodriguez, C, Calle, EE, Coates, RJ, et al. (1995) Estrogen replacement therapy and fatal ovarian cancer. Am J Epidemiol 141, 828835.
7 Albertazzi, P & Purdie, DW (2002) The nature and utility of the phytoestrogens: a review of the evidence. Maturitas 42, 173185.
8 Brzezinski, A & Debi, A (1999) Phytoestrogens: the “natural” selective estrogen receptor modulators? Eur J Obstet Gynecol Reprod Biol 85, 4751.
9 Fanti, P, Monier-Faugere, MC, Geng, Z, et al. (1998) The phytoestrogen genistein reduces bone loss in short-term ovariectomized rats. Osteoporos Int 8, 274281.
10 Scheiber, MD, Liu, JH, Subbiah, MTR, et al. (2001) Dietary inclusion of whole soy foods results in significant reductions in clinical risk factors for osteoporosis and cardiovascular disease in normal postmenopausal women. Menopause 8, 384392.
11 Song, LR & Hong, X (editors) (2001) The Grand Dictionary of Modern Traditional Chinese Medicine, pp. 19271928. Peking: People's Health Press.
12 Xie, F, Wu, CF, Zhang, Y, et al. (2005) Increase in bone mass and bone strength by Sambucus williamsii HANCE in ovariectomized rats. Biol Pharm Bull 28, 18791885.
13 Zhang, Y, Li, Q, Wan, HY, et al. (2011) Study of the mechanisms by which Sambucus williamsii HANCE extract exert protective effects against ovariectomy-induced osteoporosis in vivo. Osteoporos Int 22, 703709.
14 Yang, XJ, Wong, MS, Wang, NL, et al. (2007) Lignans from the stems of Sambucus williamsii and their effects on osteoblastic UMR106 cells. J Asian Nat Prod Res 9, 583591.
15 Yang, XJ, Wong, MS, Wang, NL, et al. (2005) Effect of phenolic acids isolated from Sambucus williamsii on proliferation and differentiation of rat osteoblastic UMR106 cells. Chin Trad Herb Drugs 36, 16041607.
16 Yang, XJ, Wang, NL, Wong, MS, et al. (2005) Studies of triterpenoids isolated from Sambucus willamsii Hance and their effects on UMR106 cell proliferation and alkaline phosphatase activity. J Shenyang Pharm Univ 22, 449452, 457.
17 Chinese Materia Medica of State Administration of Traditional Chinese Medicine (2000) Chinese Bencao, pp. 545547 [Song, LR, editor]. Shanghai: Shanghai Science and Technology Press.
18 Pang, WY, Wang, XL, Mok, SK, et al. (2010) Naringin improves bone properties in ovariectomized mice and exerts oestrogen-like activities in rat osteoblast-like (UMR-106) cells. Br J Pharmacol 159, 16931703.
19 Rachoń, D, Vortherms, T, Seidlova-Wuttke, D, et al. (2007) Effects of dietary equol on body weight gain, intra-abdominal fat accumulation, plasma lipids, and glucose tolerance in ovariectomized Sprague–Dawley rats. Menopause 14, 925932.
20 Rachoń, D, Vortherms, T, Seidlova-Wuttke, D, et al. (2007) Dietary daidzein and puerarin do not affect pituitary LH expression but exert uterotropic effects in ovariectomized rats. Maturitas 57, 161170.
21 Rachoń, D, Vortherms, T, Seidlova-Wuttke, D, et al. (2008) Effects of black cohosh extract on body weight gain, intra-accumulation, plasma lipids and glucose tolerance in ovariectomized Sprague-Dawley rats. Maturitas 60, 209215.
22 Ou, YF, Liu, Y, Xiao, HH, et al. (2009) Lignans from stems of Sambucus williamsii. China J Chin Mater Med 34, 12251227.

Keywords

Type Description Title
WORD
Supplementary Appendices

Wong Supplementary Appendices
Wong Supplementary Appendices

 Word (104 KB)
104 KB

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed