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Effects of dietary tryptophan on muscle growth, protein synthesis and antioxidant capacity in hybrid catfish Pelteobagrus vachelli♀ × Leiocassis longirostris♂

Published online by Cambridge University Press:  29 July 2021

Qin Jiang
Affiliation:
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Ye Zhao
Affiliation:
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Xiao-Qiu Zhou
Affiliation:
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Xiao-Yun Wu
Affiliation:
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Shang-Xiao Xu
Affiliation:
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Lin Feng
Affiliation:
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Yang Liu
Affiliation:
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Wei-Dan Jiang
Affiliation:
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Pei Wu
Affiliation:
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Juan Zhao
Affiliation:
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
Jun Jiang*
Affiliation:
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu611130, People’s Republic of China Animal Nutrition Institute, Sichuan Agricultural University, Chengdu611130, People’s Republic of China
*
*Corresponding author: Jun Jiang, email jjun3@foxmail.com

Abstract

The present study evaluated effects of dietary supplementation with tryptophan (Trp) on muscle growth, protein synthesis and antioxidant capacity in hybrid catfish Pelteobagrus vachelli♀ × Leiocassis longirostris♂. Fish were fed six different diets containing 2·6 (control), 3·1, 3·7, 4·2, 4·7 and 5·6 g Trp/kg diet for 56 d, respectively. Results showed that dietary Trp significantly (1) improved muscle protein content, fibre density and frequency of fibre diameter; (2) up-regulated the mRNA levels of PCNA, myf5, MyoD1, MyoG, MRF4, IGF-I, IGF-II, IGF-IR, PIK3Ca, TOR, 4EBP1 and S6K1; (3) increased phosphorylation levels of AKT, TOR and S6K1; (4) decreased contents of MDA and PC, and increased activities of CAT, GST, GR, ASA and AHR; (5) up-regulated mRNA levels of CuZnSOD, CAT, GST, GPx, GCLC and Nrf2, and decreased Keap1 mRNA level; (6) increased nuclear Nrf2 protein level and the intranuclear antioxidant response element-binding ability, and reduced Keap1 protein level. These results indicated that dietary Trp improved muscle growth, protein synthesis as well as antioxidant capacity, which might be partly related to myogenic regulatory factors, IGF/PIK3Ca/AKT/TOR and Keap1/Nrf2 signalling pathways. Finally, based on the quadratic regression analysis of muscle protein and MDA contents, the optimal Trp requirements of hybrid catfish (21·82–39·64 g) were estimated to be 3·94 and 3·93 g Trp/kg diet (9·57 and 9·54 g/kg of dietary protein), respectively.

Type
Full Papers
Copyright
© The Author(s), 2021. Published by Cambridge University Press on behalf of The Nutrition Society

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Footnotes

These authors contributed equally to this work.

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Effects of dietary tryptophan on muscle growth, protein synthesis and antioxidant capacity in hybrid catfish Pelteobagrus vachelli♀ × Leiocassis longirostris♂
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