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Biology and culture of the clown loach Chromobotia macracanthus (Cypriniformes, Cobitidae) : 1- Hormonal induced breeding, unusual latency response and egg production in two populations from Sumatra and Borneo Islands

Published online by Cambridge University Press:  25 June 2012

Marc Legendre*
Affiliation:
IRD, UMR 226 – ISEM, BP 5095, 34196 Montpellier Cedex 05, France
Darti Satyani
Affiliation:
Balai Penelitian dan Pengembangan Budidaya Ikan Hias (BP2BIH), Jl. Perikanan No. 13, Depok 41152, Indonesia
Siti Subandiyah
Affiliation:
Balai Penelitian dan Pengembangan Budidaya Ikan Hias (BP2BIH), Jl. Perikanan No. 13, Depok 41152, Indonesia
Sudarto
Affiliation:
Balai Penelitian dan Pengembangan Budidaya Ikan Hias (BP2BIH), Jl. Perikanan No. 13, Depok 41152, Indonesia
Laurent Pouyaud
Affiliation:
IRD, UMR 226 – ISEM, BP 5095, 34196 Montpellier Cedex 05, France
Etienne Baras
Affiliation:
IRD, UMR 226 – ISEM, BP 5095, 34196 Montpellier Cedex 05, France
Jacques Slembrouck
Affiliation:
IRD, UMR 226 – ISEM, BP 5095, 34196 Montpellier Cedex 05, France Balai Penelitian dan Pengembangan Budidaya Ikan Hias (BP2BIH), Jl. Perikanan No. 13, Depok 41152, Indonesia
*
a Corresponding author: Marc.Legendre@ird.fr

Abstract

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The clown loach Chromobotia macracanthus, endemic to Indonesia, is a major species on the international market of ornamental freshwater fish. In order to satisfy an increasing demand with a sustainable alternative to the massive capture of wild juveniles, research has been dedicated to the artificial propagation and domestication of this species. The present study, the first of a series, focused on favourable maintenance conditions for broodfish sexual maturation, criteria for identification of ripe fish, efficiency of hormone-induced breeding treatments, predictability of their latency response, and on the comparison of reproductive performances of fish from populations of Sumatra and Borneo Islands (in total, 112 females of 46 to 404 g body weight). When reared in fully controlled conditions in large water recirculation systems, broodfish originating from Sumatra had reproductive performances similar to or slightly higher than those maturing in the wild (ovulation rate of 93% vs. 82%, relative fecundity of 109 277 vs. 103 550 ova  kg-1 and fertilization rate of 73% vs. 61%, respectively). In the same rearing conditions, captive females from Borneo (n = 22) showed lower ovulation rate (77%), relative fecundity (76 262 ova kg-1) and fertilization rate (50%) than those originating from Sumatra (n = 28). By contrast, the mean individual weight of ova (around 0.8 mg) was independent from the origin or maintenance conditions of females. An initial modal follicle diameter  ≥1.02 mm generally led to high ovulation success (>80%) after hormonal treatment and is recommended as the main criterion for selecting female broodfish. Two hormonal treatments for inducing oocyte maturation and ovulation (T1: two successive injections of Ovaprim at a 6 h-interval; T2: one injection of human chorionic gonadotropin (hCG)- and one of Ovaprim 24 h later), produced similar results in terms of ovulation rate, quantity and quality of ova collected. With both treatments, the latency decreased with increasing water temperature, then increased again at temperatures  >28–29 °C. To our knowledge, such U-shaped relationship between the latency response and temperature has never been documented in teleost fishes.

Type
Research Article
Copyright
© EDP Sciences, IFREMER, IRD 2012

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