Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-21T18:12:41.538Z Has data issue: false hasContentIssue false
  • English
  • Français

Iron stress and genetic response in cyanobacteria : fur genes from Synechococcus PCC 7942 and Anabaena PCC 7120

Published online by Cambridge University Press:  15 March 2009

J. A. Hernández ,
M. Artieda ,
M. L. Peleato ,
M. F. Fillat  and
M. T. Bes
J. A. Hernández
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. Artieda
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. L. Peleato
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. F. Fillat
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
M. T. Bes
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular. Facultad de Ciencias. 50009 - Zaragoza, Spain
Get access

Abstract

Fur (Ferric uptake regulator) is a DNA-binding protein which represses the transcription of iron-regulated promoters using Fe2+ as a cofactor. In Gram negative bacteria, fur controls the expression of toxins and virulence factors when facing iron-stress. Iron is a key element limiting the growth and proliferation of phytoplankton. We have cloned the fur-gene from the cyanobacterium Synechococcus PCC 7942, applying the polymerase chain reaction (PCR) technique. The DNA fragments amplified encodes a Fur-type protein, which conserves the main motifs found in the family of Fur proteins. Recombinant Fur protein was successfully overproduced, reaching about 15 % of the total protein content. Also, a Fur protein was identified in the filamentous, nitrogen-fixing cyanobacterium Anabaena PCC 7120.

Keywords

ferric uptake regulator (fur)ironcyanobacteriaferric uptake regulator (fur)fercyanobactérie
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 38 , Issue 1 , March 2002 , pp. 3 - 11
Copyright
© Université Paul Sabatier, 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)