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Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits

  • M. Kovács (a1) (a2), G. Tornyos (a1), Zs. Matics (a2), L. Kametler (a1), V. Rajli (a1), Zs. Bodnár (a1), M. Kulcsár (a3), Gy. Huszenicza (a3), Zs. Keresztes (a2) and S. Cseh (a3)...


Pannon White (n = 12) male rabbits (weight: 4050 to 4500 g, age: 9 months) received 2 ml of a suspension containing purified T-2 toxin by gavage for 3 days. The daily toxin intake was 4 mg/animal (0.78 to 0.99 mg/kg body weight (BW)). Control animals (n = 12) received toxin-free suspension for 3 days. Since a feed-refusal effect was observed on the second day after T-2 administration, a group of bucks (n = 10) were kept as controls (no toxin treatment) but on a restricted feeding schedule, that is, the same amount of feed was provided to them as was consumed by the exposed animals. On day 51 of the experiment (i.e. 48 days after the 3-day toxin treatment), semen was collected, and pH, concentration, motility and morphology of the spermatozoa, as well as concentration of citric acid, zinc and fructose in the seminal plasma, were measured. After gonadotropin-releasing hormone (GnRH) analogue treatment, the testosterone level was examined. One day of T-2 toxin treatment dramatically decreased voluntary feed intake (by 27% compared to control, P < 0.05) and remained lower (P < 0.05) during the first 2 weeks after the withdrawal of the toxin. BW of the contaminated rabbits decreased by 88% on days 17 and 29 compared to controls (P < 0.05). No effect of toxin treatment was detected on pH and quantity of the semen or concentration of spermatozoa. The ratio of spermatozoa showing progressive forward motility decreased from 65% to 53% in the semen samples of toxin-treated animals compared to controls (P > 0.05). The ratio of spermatozoa with abnormal morphology increased (P < 0.05) in the ejaculates collected from the toxin-treated animals. T-2 toxin applied in high doses decreased the concentration of citric acid in seminal plasma (P < 0.05). No effect of T-2 toxin on the concentrations of the other seminal plasma parameters (fructose and zinc) was observed. T-2 toxin decreased the basic testosterone level by 45% compared to control (P < 0.01) and resulted in lower (P < 0.05) GnRH-induced testosterone concentration. Feed restriction, that is, less nutrient intake, resulted in more morphologically abnormal spermatozoa in the semen, but it did not cause significant loss in BW, motility of the spermatozoa, composition of the seminal plasma or testosterone concentration – its effect needs further examination.


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Assane, M, Katiellou, MD, Sere, A, Gongnet, P, Missohou, A 1995. Effect of partial restriction of drinking-water output and reproduction performance in rabbit. Revue de Medecine Veterinaire 146, 427432.
Barnett, CIR, Tomlinson, MJ, Cooke, ID 1993. Prognostic significance of computerized motility analysis for in vivo fertility. Fertility and Sterility 60, 520525.
Bradbury, J 1997. It's not looking good for sperm counts. Lancet 350, 1605.
Brecchia, G, Bonanno, A, Galeati, G, Federici, C, Maranesi, M, Gobbetti, A, Zerani, M, Boiti, C 2006. Hormonal and metabolic adaptation to fasting: effects on the hypothalamic–pituitary–ovarian axis and reproductive performance of rabbit does. Domestic Animal Endocrinology 31, 105122.
Chapin, RE, Gulati, DK, Barnes, LH, Teague, JL 1993. The effects of feed restriction on reproductive function in Sprague-Dawley rats. Toxicological Sciences 20, 2329.
Cooper, TG, Yeung, CH 2000. Physiology of sperm maturation and fertilization. In Andrology. Male reproductive health and dysfunction (ed. E Nieschlag and HM Behre), pp. 6379, 2nd edition. Springer, Berlin, Germany.
Csernus, V 1982. Antibodies of high affinity and specificity for RIA determination of progesterone, testosterone, estradiol-17β and cortisol. In Advances in steroid analysis. I (ed. S Görög), pp. 171177. Academic Press, Budapest, Hungary.
Egbunike, GN 1982. Steroidogenic and spermatogenic potentials of the male rat after acute treatment with aflatoxin B1. Andrologia 14, 440446.
Ettlin, RA, Bechter, R, Lee, IP, Hodel, C 1984. Aspects of testicular toxicity induced by anticancer drugs. In disease, metabolism and reproduction in the toxic response to drugs and other chemicals. Archives of Toxicology (suppl. 7), 151154.
Fekete, S, Huszenicza, G 1993. Effects f T-2 toxin on ovarian activity and some metabolic variables of rabbits. Laboratory Animal Science 43, 646649.
Fekete, S, Tamas, J, Vanyi, A, Glávits, R, Bata, A 1989a. Effect of T-2 toxin on feed intake digestion and pathology of rabbits. Laboratory Animal Science 39, 603606.
Fekete, S, Tamas, J, Vanyi, A, Glávits, R, Bata, A 1989b. Effect of T-2 toxin fed in sublethal quantity on digestion in the rabbit [in Hungarian]. Magyar Állatorvosok Lapja 44, 739740.
Fenske, M, Fink-Gremmels, J 1990. Effect of fungal metabolites on testosterone secretion in vitro. Archives of Toxicology 64, 7275.
Fodor, J, Németh, M, Kametler, L, Pósa, R, Kovács, M, Horn, P 2006. Novel methods of Fusarium toxins’ production for toxicological experiments. Methodological study. Acta Agraria Kaposvariensis 10, 277285.
Gillan, L, Kroetsch, T, Maxwell, WMC, Evans, G 2008. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Animal Reproduction Science 103, 201214.
Glávits, R, Vanyi, A, Fekete, S, Tamas, J 1989. Acute toxicological experiment of T-2 toxin in rabbits. Acta Veterinaria Hungarica 37, 7579.
Ibeh, IN, Saxena, DK 1998. Effect of alpha-tocopherol supplementation on the impact of aflatoxin B1 of the testis of rats. Experimental and Toxicologic Pathology 50, 221234.
Ibeh, IN, Uraih, N, Ogonar, JI 1994. Dietary exposure to aflatoxin in human male infertility in Benin city, Nigeria. International Journal of Fertility and Menopausal Studies 39, 208214.
Kendall, NR, McMullen, S, Green, A, Rodway, RG 2000. The effect of a zinc, cobalt, and selenium soluble glass bollus on trace element status and semen quality of ram lambs. Animal Reproduction Science 62, 277283.
Krause, W 1995. Computer-assisted semen analysis systems: comparison with routine evaluation and prognostic value in male fertility and assisted reproduction. Human Reproduction 10, 6066.
Krausz, C, Forti, G 2000. Clinical aspects of male infertiliy. In The genetic basis of male infertility (ed. K McElreavey), pp. 121. Springer, Heidelberg, Germany.
de Kretser, DM, Kerr, JB 1994. The cytology of testis. In The physiology of reproduction (ed. E Knobil and JD Neill), pp. 11171290. Raven Press Ltd, New York, USA.
Kruger, TF, Acosta, AA, Simmons, KF, Swanson, RJ, Matta, JF, Oehninger, S 1988. Predictive value of abnormal sperm morphology. In in vitro fertilization. Fertility and Sterility 49, 112117.
Nikodémusz, E, Mézes, M 1992. Subchronic toxic effects of dietary T-2 toxin in breeding geese. Proceedings of the 9th International Symposium on Water Fowl, Pisa, Italy, pp. 201–203.
Niyo, KA, Richard, JL, Niyo, Y, Tiffany, LH 1988. Pathologic, hematologic and serologic changes in rabbits given T-2 mycotoxin orally and exposed to aerosols of Aspergillus fumigatus conidia. American Journal of Veterinary Research 49, 21512160.
Parkinson, T 2009. Normal reproduction in male animals. In Veterinary reproduction and obstetrics (ed. DE Noakes, TJ Parkinson and GCW England), pp. 681705, 9th edition. Saunders Elsevier, London, UK.
Sándor, G, Ványi, A 1990. Mycotoxin research in the Hungarian Central Veterinary Institute. Acta Veterinaria Hungarica 38, 6168.
Sawyer, DE, Hillman, GR, Uchida, T, Brown, DB 1998. Altered nuclear activation parameters of rat sperm treated in vitro with chromatin-damaging agents. Toxicological Science 44, 5262.
Schlatter, J 2004. Toxicity data relevant for hazard characterization. Toxicology Letters 153, 8389.
Scientific Committee on Food (SCF) 2001. Opinion of the scientific committee on food on Fusarium toxins. Part 5: T-2 toxin and HT-2 toxin. European Commission, Brussels, Belgium. Retrieved January 4, 2011, from
Scientific Committee on Food (SCF) 2002. Opinion of the Scientific Committee on food on fusarium toxins. Part 6: group evaluation of T-2 toxin, HT-2 toxin, nivalenol and deoxynivalenol. European Commission, Brussels, Belgium. Retrieved January 4, 2011, from
Scott, PM 1990. Trichothecenes in grains. Cereal Foods World 35, 661666.
Smith, TK 1992. Recent advances in the understanding of Fusarium trichothecene mycotoxicoses. Journal of Animal Science 70, 39893993.
Sprando, RL, Collins, TFX, Black, TN, Olejnik, N, Rorie, JI, Eppley, RM, Ruggles, DI 2005. Characterization of the effect of deoxynivalenol on selected male reproductive endpoints. Food and Chemical Toxicology 43, 623635.
Statistical Package for the Social Sciences (SPSS) for Windows (Microsoft) 2002. Inc. Version 10.0.
Steinberger, A, Klinefelter, G 1993. Sensitivity of Sertoli and Leydig cells to xenobiotics in in vitro models. Reproductive Toxicology 7, 2337.
Sundstøl, GE, Pettesson, H 2004. Toxicological evaluation of trichothecenes in animal feed. Animal Feed Science and Technology 114, 205239.
Szilágyi, M, Fekete, S, Huszenicza, Gy, Albert, M 1994. Biochemical and physiological effects of long-term sublethal T-2 toxin feeding in rabbits. Acta Biologica Hungarica 45, 6976.
Wannemacher, RW, Wiener, SL 1997. Trichothecene mycotoxins. In Medical aspects of chemical and biological wearforce. Textbook of military medicine (ed. R Zajtchuk). pp. 655677. Burden Institute, Washington DC, USA.
Weinbauer, GF, Gromoll, J, Simoni, M, Nieschlag, E 2000. Physiology of testicular function. In Andrology. Male reproductive health and dysfunction, 2nd edition (ed. E Nieschlag and HM Behre), pp. 2363. Springer, Berlin, Germany.
World Health Organisation (WHO) 1990. International programme on chemical safety. environmental health criteria 105. Selected Mycotoxins: Ochratoxins, Trichothecens, Ergot. Geneva, Switzerland. Retrieved January 4, 2011, from
World Health Organisation (WHO) 1999. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. WHO, 4th edition. Cambridge University Press, UK p. 138.
Yeung, CH 1995. Development of sperm motility. In Frontiers in endocrinology. Epididymis: role and importance in the infertility treatment (ed. S Hamanah, R Mieusset and JL Dacheux), pp. 7386. Ares Serono Symposia, Rome, Italy.



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