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Single nucleotide polymorphisms in the growth hormone and IGF type-1 (IGF1) genes associated with carcass traits in Santa Ines sheep

  • A. N. Meira (a1), H. Montenegro (a2), L. L. Coutinho (a2), G. B. Mourão (a2), H. C. Azevedo (a3), E. N. Muniz (a3), A. L. Machado (a1), L. P. Sousa-Jr (a1), V. B. Pedrosa (a4) and L. F. B. Pinto (a1)...

Abstract

Polymorphisms in the growth hormone (GH) and IGF type-1 (IGF1) genes have been associated with the economic traits in farm animals, including BW of some sheep breeds. However, it remains unknown if these polymorphisms also affect carcass traits in sheep. Thus, we aimed to identify polymorphisms in the GH and IGF1 genes in Santa Ines sheep in order to describe their allelic and genotypic frequencies as well as to test the hypotheses that they are associated with the carcass traits. Fragments of 4550 bp (IGF1) and 1194 bp (GH) were sequenced in up to 191 lambs. In all, 18 polymorphisms were identified in the IGF1 and 21 in the GH gene. The IGF1 polymorphisms rs430457475, rs412470350, rs409110739 and rs400113576 showed an additive effect on the internal carcass length (−0.9265±0.4223), rump girth (−2.9285±1.1473), rib yield (−1.0003±0.4588) and neck weight (−0.0567±0.0278), respectively. In addition, the polymorphisms rs58957314 in the GH affected the rib weight (−0.4380±0.1272) and rib yield (−2.2680±0.6970), loin weight (−0.1893±0.0516) and loin yield (−0.9423±0.3259), palette weight (−0.2265±0.0779) and palette yield (−0.9424±0.4184), leg weight (−0.3960±0.1375), neck weight (−0.0851±0.0394) and carcass finishing score (−0.1700±0.0839). These results allow us to conclude that there are polymorphisms in the IGF1 and GH genes associated with carcass traits in Santa Ines sheep, which can provide important information for marker-assisted selection.

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Abdelmoneim, TS, Brooks, PH, Afifi, M and Swelum, AAA 2017. Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep. Indian. Journal of Animal Research 51, 205211.
Cauveri, D, Sivaselvam, SN, Karthickeyan, SMK, Tirumurugaan, KG, Kumanan, K and Venkataramanan, R 2016. Single nucleotide polymorphisms in GH (growth hormone) gene associated with growth traits in Nilagiri sheep of Tamil Nadu. International Journal of Science, Environment and Technology 5, 40974103.
Dettori, M, Pazzola, M, Pira, E, Paschino, P and Vacca, G 2015. The sheep growth hormone gene polymorphism and its effects on milk traits. Journal of Dairy Research 82, 169176.
Devesa, J, Almengló, C and Devesa, P 2016. Multiple effects of growth hormone in the body: is it really the hormone for growth? Clinical Medicine Insights: Endocrinology and Diabetes 2016, 4771.
Farag, IM, Darwish, AM, Darwish, HR, AbdelAziz, KB, Ramadan, WA, Mohamed, MI and Othman, EO 2016. Polymorphism of growth hormone gene and its association with wool traits in Egyptian sheep breeds. African Journal of Biotechnology 15, 549556.
Gorlov, IF, Kolosov, YA, Shirokova, NV, Getmantseva, LV, Slozhenkina, MI, Mosolova, NI, Bakoev, NF, Leonova, MA, Kolosov, AY and Zlobina, EY 2017. Association of the growth hormone gene polymorphism with growth traits in Salsk sheep breed. Small Ruminant Research 150, 1114.
Han, YC, Sun, YG and Li, Q 2016. Growth hormone polymorphisms and growth traits in Chinese Tibetan sheep Ovis aries. Genetics and Molecular Research 15, GMR15038397.
He, JN, Zhang, BY, Chu, MX, Wang, PQ, Feng, T, Cao, GL, Di, R, Fang, L, Huang, DW, Tang, QQ and Li, N 2012. Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep. Molecular Biology Reports 39, 98019807.
Islam, KK, Vinsky, M, Crews, RE, Okine, E, Moore, SS, Crews-JR, DH and Li, C 2009. Association analyses of a SNP in the promoter of IGF1 with fat deposition and carcass merit traits in hybrid, Angus, and Charolais beef cattle. Animal Genetics 40, 766769.
Jia, JL, Zhang, LP, Wu, JP, Ha, ZJ and Li, WW 2014. Study of the correlation between GH gene polymorphism and growth traits in sheep. Genetics and Molecular Research 13, 71907200.
Jucá, AF, Faveri, JC, Melo-Filho, GM, Ribeiro-Filho, AL, Azevedo, HC, Muniz, EN, Pedrosa, VB and Pinto, LFB 2016. Effects of birth type and family on the variation of carcass and meat traits in Santa Ines sheep. Tropical Animal Health and Production 48, 435443.
Jucá, AF, Faveri, JC, Melo-Filho, GM, Ribeiro-Filho, AL, Azevedo, HC, Muniz, EM and Pinto, LFB 2014. Performance of the Santa Ines breed raised on pasture in semiarid tropical regions and factors that explain trait variation. Tropical Animal Health and Production 46, 12491256.
Kumari, R, Kumar, R, Meena, AS, Jyotsana, B, Prince, LLL and Kumar, S 2014. Genetic polymorphism of growth hormone gene in native sheep breeds of India. The Indian Journal of Small Ruminant 20, 1518.
Langmead, B and Salzberg, SL 2012. Fast gapped-read alignment with Bowtie 2. Nature Methods 4, 357359.
Li, H and Durbin, R 2009. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 14, 17541760.
Malewa, AD, Hakim, L, Maylinda, S and Husain, MH 2014. Growth hormone gene polymorphisms of Indonesia fat tailed sheep using PCRRFLP and their relationship with growth traits. Livestock Research for Rural Development 26, 115.
McManus, C, Paludo, GR, Louvandini, H, Gugel, R, Sasaki, LCB and Paiva, SR 2009. Heat tolerance in Brazilian sheep: physiological and blood parameters. Tropical Animal Health and Production 41, 95101.
Mexia, AA, De-Macedo, FAF, De-Oliveira, CAL, Zundts, M, Yamamoto, SM, Santello, GA, Carneiro, RDC and Sasa, A 2011. Susceptibilidade a nematóides em ovelhas Santa Inês, Bergamácia e Texel no Noroeste do Paraná. Semina: Ciências Agrárias 32, 19211928.
Moradian, C, Mohamadi, N, Razavi-Sheshdeh, SA, Hajihosseinlo, A and Ashrafi, F 2013. Effects of genetic polymorphism at the growth hormone gene on growth traits in Makooei sheep. European Journal of Experimental Biology 3, 101105.
Nasution, D, Arnim, A, Jamsari, A and Syofyan, Y 2017. Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province. African Journal of Biotechnology 16, 11591167.
Nazari, F, Noshary, A and Hemati, B 2016. Association between insulin–like growth factor i polymorphism and early growth traits in Iranian Zandi sheep, found polymerase chain reaction restriction fragment length polymorphism. Iranian Journal of Applied Animal Science 6, 665669.
Ng, CPE and Henikoff, S 2003. SIFT: predicting amino acid changes that affect protein function. Nucleic Acids Research 31, 38123814.
Oliveira, MCDS, Regitano, LCA, Roese, AD, Anthonisen, DG, Patrocínio, E, Parma, MM, Scagliusi, SMM, Timóteo, WHB and Jardim, SN 2007. Fundamentos teórico-práticos e protocolos de extração e de amplificação de DNA por meio da técnica de reação em cadeia da polimerase. EMBRAPA. Retrieved on 07 October 2016 from http://www.alice.cnptia.embrapa.br/bitstream/doc/48295/1/LivroProtMolecular.pdf.
Pérez-Enciso, M and Misztal, I 2011. Qxpak5: Old mixed model solutions for new genomics problems. BMC Bioinformatics 12, 202.
Statistical Analysis System 2004. SAS/STAT user’s guide: version 9.1. SAS Institute, Cary, NC, USA.
Scatà, MC, Catillo, G, Annicchiarico, G, De-Matteis, G, Napolitano, F, Signorelli, F and Moioli, B 2010. Investigation on lactation persistency and IGF-I gene polymorphisms in dairy sheep. Small Ruminant Research 89, 711.
Seevagan, M, Jeichitra, V, Rajendran, R and Tirumurugaan, KG 2015. Detection of lethal SNP (A781G) in growth hormone (GH) gene of Indian sheep. Small Ruminant Research 126, 1315.
Su, R, Sun, W, Li, D, Wang, QZ, Lv, XY, Musa, HH, Chen, L, Zhang, YF and Wu, WZ 2014. Association between DLK1 and IGF-I gene expression and meat quality in sheep. Genetics and Molecular Research 13, 1030810319.
Sun, W, Su, R, Li, D, Musa, HH, Kong, Y, Ding, JT, Ma, YH, Chen, L, Zhang, YF and Wu, WZ 2014. Developmental changes in IGF-I and MyoG gene expression and their association with meat traits in sheep. Genetics and Molecular Research 13, 27722783.
Trukhachev, V, Skripkin, V, Kvochko, A, Kulichenko, A, Kovalev, D, Pisarenko, S, Volynkina, A, Selionova, M, Aybazov, M, Shumaenko, S, Omarov, A, Mamontova, T, Yatsyk, O and Krivoruchko, A 2016. Polymorphisms of the IGF1 gene in Russian sheep breeds and their influence on some meat production parameters. Slovenian Veterinary Research 53, 7783.
Zhbannikov, IY, Hunter, SS and Settles, ML 2013. SEQYCLEAN user manual. Retrieved on 13 December 2016 from http://sourceforge.net/projects/seqclean/files/.

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