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Reference values for biochemical parameters in blood serum of young and adult alpacas (Vicugna pacos)

  • T. Husakova (a1), L. Pavlata (a2), A. Pechova (a3), K. Hauptmanova (a1), E. Pitropovska (a1) and L. Tichy (a4)...


The aim of this study was to establish reference interval for biochemical parameters in blood of alpacas on the basis of large population of clinically healthy animals, and to determine the influence of sex, age and season on nitrogen and lipid metabolites, enzymes, electrolytes, vitamins and minerals in blood of alpacas. Blood samples were collected from 311 alpacas (61 males and 201 females >6 months of age and 49 crias (21 males and 28 females) ⩽6 months of age). Selected farms were located in Central Europe (Czech Republic and Germany). We determined 24 biochemical parameters from blood serum. We performed the comparison of results by the sex of animals and for the older group also the comparison of the results with regard to the season, respectively, to the feeding period. We found no highly significant difference (P<0.01) between males and females with the exception of γ-glutamyl transferase (GGT), alkaline phosphatase (ALP) and cholesterol. We found 15 significantly different parameters between the group of crias 6 months of age and the older alpacas. Based on our findings we suggest for most parameters to use different reference intervals (especially ALP, cholesterol, total protein, globulin, non-esterified fatty acids (NEFA), GGT and phosphorus) for the two above-mentioned age groups. Another important finding is the differences between some parameters in older group of alpacas in summer/winter feeding period. Animals in the summer feeding period have higher values of parameters related to fat mobilization (β-hydroxybutyrate, NEFA) and liver metabolism (bilirubin, alanine aminotransferase). The winter period with increased feeding of supplements with higher amount of fat, vitamins and minerals is characteristic by increased values of cholesterol, triglycerides, vitamins A and E, and some minerals (K, Ca, Mg and Cl) in blood serum. Clinical laboratory diagnosis of metabolic disturbances may be improved with use of age-based reference values and with consideration of seasonal differences.


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Bouda, J, Jagos, P and Dvorak, V 1980. Fluorometric determination of vitamins A and E in blood plasma, colostrum and the liver of cattle (in Czech). Ceskoslovenska Fysiologie 29, 351.
Burton, S, Robinson, TF, Roeder, BL, Johnston, NP, Latorre, EV, Reyes, SB and Schaajle, B 2003. Body condition and blood metabolite characterization of alpaca (Lama pacos) three months prepartum and offspring three months postpartum . Small Ruminant Research 48, 6976.
Dart, AJ, Kinde, H, Hodgson, DR, Peauroi, JR, Selby, AW, Maas, J and Fowler, ME 1996. Serum α-tocopherol, vitamin A and blood selenium concentration and glutathione peroxidase activity in llamas fed alfalfa hay. American Journal of Veterinary Research 57, 689692.
Dawson, DR, DeFrancisco, RJ, Mix, SD and Stokol, T 2011. Reference intervals for biochemical analytes in serum and heparinized plasma and serum protein fractions in adult alpacas (Vicugna pacos). Veterinary Clinical Pathology 40, 538548.
Foster, A, Bidewell, C, Barnett, J and Sayers, R 2009. Haematology and biochemistry in alpacas and llamas. In Practice 31, 276281.
Fowler, ME 2010. Medicine and surgery of camelids, 3rd edition Wiley-Blackwell, Iowa, USA.
Gauly, M, Maier, H and Trah, M 1998. Blood collection, haematological values, biochemical parameters of new world camelids. Tierarztliche Umschau 53, 751754.
Hajduk, P 1992. Haematological reference values for alpacas. Australian Veterinary Journal 69, 8990.
Hengrave Burri, IH, Tschudi, P, Martig, J, Liesegang, A and Meylan, M 2005. South American camelids in Switzerland. Reference values for blood parameters. Schweizer Archiv fur Tierheilkunde 147, 335343.
Herdt, TH 1995. Blood serum concentrations of selenium of female llamas (Lama glama) in relationship to feeding practices, regions of United States, reproductive stage, and health of offspring. Journal of Animal Science 73, 337344.
Herdt, TH and Stowe, HD 1991. Fat-soluble vitamin nutrition for dairy cattle. Veterinary Clinics of North America: Food Animal Practice 7, 391415.
Khan, ZI, Hussain, A, Ashraf, M, Ashraf, MY and McDowell, LR 2007. Macromineral status of grazing sheep in a semi-arid region of Pakistan. Small Ruminant Research 68, 279284.
McDowell, LR, Williams, SN, Hidiroglou, N, Njeru, CA, Hill, GM, Ochoa, L and Wilkinson, NS 1996. Vitamin E supplementation for the ruminant. Animal Feed Science and Technology 60, 273296.
Morgante, M, Manuali, E, Schiano, C and Ranucci, S 2001. Blood mineral concentrations, and erythrocyte glutathione peroxidase activity in adult female alpacas raised in central Italy. In Progress in South American camelids research (ed. M Gerke and C Renieri), pp. 281285. EAAP European Association for Animal Production Publication, Wageningen Academic Publishers, the Netherlands.
Pechova, A, Dvorak, R, Drastich, P, Lubojacka, V, Pavlata, L and Poul, J 2006. Influence of increased lipid content in diet in the form of treated rapeseed meal on the metabolism and milk yield of dairy cows in the first third of lactation. Veterinarni Medicina 51, 346355.
Podhorsky, A, Pechova, A, Dvorak, R and Pavlata, L 2007. Metabolic disorders in dairy calves in postpartum period. Acta Veterinaria Brno 76, S45S53.
Smith, BB and Van Saun, RJ 2001. Seasonal changes in serum calcium, phosphorus, and vitamin D concentrations in llamas and alpacas. American Journal of Veterinary Research 62, 11871193.
Smith, BB, Van Saun, RJ, Reed, PJ, Craig, AM and Youngberg, A 1998. Blood mineral and vitamin E concentrations in llamas. American Journal of Veterinary Research 59, 10631070.
Thompson, SY, Erdody, P and Maxwell, WB 1973. Simultaneous fluorometric determinations of vitamins A and E in human serum and plasma. Biochemical Medicine 8, 403414.
Van Saun, RJ 2008. Selenium nutrition in camelids – part 2. 3, 2630.
Van Saun, RJ 2009. Nutritional diseases of llamas and alpacas. Veterinary Clinics of North America: Food Animal Practice 25, 797810.


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Reference values for biochemical parameters in blood serum of young and adult alpacas (Vicugna pacos)

  • T. Husakova (a1), L. Pavlata (a2), A. Pechova (a3), K. Hauptmanova (a1), E. Pitropovska (a1) and L. Tichy (a4)...


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