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Rabbit SLC15A1, SLC7A1 and SLC1A1 genes are affected by site of digestion, stage of development and dietary protein content

  • L. Liu (a1), H. Liu (a1), L. Ning (a1) and F. Li (a1)


Peptide transporter 1 (SLC15A1, PepT1), excitatory amino acid transporter 3 (SLC1A1, EAAT3) and cationic amino acid transporter 1 (SLC7A1, CAT1) were identified as genes responsible for the transport of small peptides and amino acids. The tissue expression pattern of rabbit (SLC15A1, SLC7A1 and SLC1A1) across the digestive tract remains unclear. The present study investigated SLC15A1, SLC7A1 and SLC1A1 gene expression patterns across the digestive tract at different stages of development and in response to dietary protein levels. Real time-PCR results indicated that SLC15A1, SLC7A1 and SLC1A1 genes throughout the rabbits’ entire development and were expressed in all tested rabbit digestive sites, including the stomach, duodenum, jejunum, ileum, colon and cecum. Furthermore, SLC7A1 and SLC1A1 mRNA expression occurred in a tissue-specific and time-associated manner, suggesting the distinct transport ability of amino acids in different tissues and at different developmental stages. The most highly expressed levels of all three genes were in the duodenum, ileum and jejunum in all developmental stages. All increased after lactation. With increased dietary protein levels, SLC7A1 mRNA levels in small intestine and SLC1A1 mRNA levels in duodenum and ileum exhibited a significant decreasing trend. Moreover, rabbits fed a normal level of protein had the highest levels of SLC15A1 mRNA in the duodenum and jejunum (P<0.05). In conclusion, gene mRNA differed across sites and with development suggesting time and sites related differences in peptide and amino acid absorption in rabbits. The effects of dietary protein on expression of the three genes were also site specific.


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Abdou, NI and Richter, M 1970. Cells involved in the immune response XVIII. Cells involved in the immune response. 18. Potential hemolysin-forming cells in the sacculus rotundus of the normal adult rabbit. The Journal of Immunology 104, 10871093.
Apps, DK 1991. Channels, carriers, and pumps: an introduction to membrane transport. Febs Letters 287, 316.
Bellier, R, Gidenne, T, Vernay, M and Colin, M 1995. In vivo study of circadian variations of the cecal fermentation pattern in postweaned and adult rabbits. Journal of Animal Science 73, 128135.
Broer, A, Wagner, C, Lang, F and Broer, S 2000. The heterodimeric amino acid transporter 4F2hc/y+ LAT2 mediates arginine efflux in exchange with glutamine. Biochemical Journal 349, 787795.
Brosnan, JT and Brosnan, ME 2013. Glutamate: a truly functional amino acid. Amino Acids 45, 413418.
Carabaño, R, Fraga, MJ, Santoma, G and de Blas, JC 1988. Effect of diet on composition of cecal contents and on excretion and composition of soft and hard feces of rabbits. Journal of Animal Science 66, 901910.
Chen, H, Wong, EA and Webb, KE Jr 1999. Tissue distribution of a peptide transporter mRNA in sheep, dairy cows, pigs, and chickens. Journal of Animal Science 77, 12771283.
Corzo, A, Loar, RE, Kidd, MT and Burgess, SC 2011. Dietary protein effects on growth performance, carcass traits and expression of selected jejunal peptide and amino acid transporters in broiler chickens. Revista Brasileira de Ciência Avícola 219, 139146.
Daniel, H and Kottra, G 2004. The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology. Pflügers Archiv 447, 610618.
Erickson, RH, Gum, JR, Lindstrom, MM, McKean, D and Kim, YS 1995. Regional expression and dietary regulation of rat small intestinal peptide and amino acid transporter mRNAs. Biochemical and Biophysical Research Communications 216, 249257.
Fan, MZ, Matthews, JC, Etienne, NMP, Barbara, S, Dale, L and Burrin, DG 2004. Expression of apical membrane L-glutamate transporters in neonatal porcine epithelial cells along the small intestinal crypt-villus axis. American Journal of Physiology-Gastrointestinal and Liver Physiology 287, 385398.
Feng, Y, Duan, CJ, Pang, H, Mo, XC, Wu, CF, Yu, Y, Hu, YL, Wei, J, Tang, JL and Feng, JX 2007. Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases. Applied Microbiology and Biotechnology 75, 319328.
Gilbert, ER, Li, H, Emmerson, DA, Webb, KE Jr and Wong, EA 2008. Dietary protein quality and feed restriction influence abundance of nutrient transporter mRNA in the small intestine of broiler chicks. The Journal of Nutrition 138, 262271.
Gilbert, ER, Li, H, Emmerson, DA, Webb, KE Jr and Wong, EA 2007. Developmental regulation of nutrient transporter and enzyme mRNA abundance in the small intestine of broilers. Poultry Science 86, 17391753.
Gregory, NG and Wotton, SB 1984. Sheep slaughtering procedures. II. Time to loss of brain responsiveness after exsanguination or cardiac arrest. British Veterinary Journal 140, 354360.
Hatzoglou, M, Fernandez, J, Yaman, I and Closs, E 2004. Regulation of cationic amino acid transport: the story of the CAT-1 transporter. Annual Review of Nutrition 24, 377399.
Hyde, R, Taylor, PM and Hundal, HS 2003. Amino acid transporters: roles in amino acid sensing and signaling in animal cells. Biochemical Journal 373, 118.
Lin, M, Zhang, B, Yu, C, Li, J, Zhang, L, Sun, H, Gao, F and Zhou, G 2014. L-Glutamate supplementation improves small intestinal architecture and enhances the expressions of jejunal mucosa amino acid receptors and transporters in weaning piglets. PLoS One 9, e111950.
Liu, L, Song, Z, Jiao, H and Lin, H 2014. Glucocorticoids increase NPY gene expression via hypothalamic AMPK signaling in broiler chicks. Endocrinology 155, 21902198.
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods 25, 402408.
Moundras, C, Remesy, C and Demigne, C 1993. Dietary protein paradox: decrease of amino acid availability induced by high-protein diets. American Journal of Physiology-Gastrointestinal and Liver Physiology 264, 10571065.
Nicodemus, N, Mateos, J, de Blas, JC and Carabaño, R 1999. Effect of diet on amino acid composition of soft faeces and the contribution of soft faeces to total amino acid intake, through caecotrophy in lactating doe rabbits. Animal Science 69, 167170.
Nielsen, CU, Amstrup, J, Steffansen, B, Frokjaer, S and Brodin, B 2001. Epidermal growth factor inhibits glycylsarcosine transport and hSLC15A1 expression in a human intestinal cell line. American Journal of Physiology-Gastrointestinal and Liver Physiology 281, 191199.
Padilha, MTS, Licois, D, Gidenne, T, Carré, B and Fonty, G 1995. Relationships between microflora and caecal fermentation in rabbits before and after weaning. Reproduction Nutrition Development 35, 375386.
Rubio-Aliaga, I and Daniel, H 2008. Peptide transporters and their roles in physiological processes and drug disposition. Xenobiotica 38, 10221042.
Speier, JS, Yadgary, L, Uni, Z and Wong, EA 2012. Gene expression of nutrient transporters and digestive enzymes in the yolk sac membrane and small intestine of the developing embryonic chick. Poultry Science 91, 19411949.
Shen, H, Smith, DE and Brosius, FC 2001. Developmental expression of SLC15A1 and PEPT2 in rat small intestine, colon, and kidney. Pediatric Research 49, 789795.
Shiraga, T, Miyamoto, K, Tanaka, H, Yamamoto, H, Taketani, Y, Morita, K, Tamai, I, Tsuji, A and Takeda, E 1999. Cellular and molecular mechanisms of dietary regulation on rat intestinal H/peptide transporter SLC15A1. Gastroenterology 116, 354362.
Sun, YJ, Ni, ZH, Chen, YL, Zhang, NP and Wang, YJ 2017. Study on distribution of free amino acid and small peptide and the expression of related transporter genes in gastrointestinal tract of sheep. Acta Veterinaria et Zootechnica Sinica 48, 462473.
Van Beers, EH, Büller, HA, Grand, RJ, Einerhand, AW and Dekker, J 1995. Intestinal brush border glycohydrolases: structure, function, and development. Critical Reviews in Biochemistry and Molecular Biology 30, 197262.
Wang, XJ, Xu, SH, Liu, L, Song, ZG, Jiao, HC and Lin, H 2017. Dietary fat alters the response of hypothalamic neuropeptide Y to subsequent energy intake in broiler chickens. Journal of Experimental Biology 220, 607614.
Wu, LN 2016. The influence of α-spinasterol on the expression of P-glycoprotein and PepTl on the small intestine of mice. Master thesis, Sichuan Agricultural University, Chengdu, China.
Xu, QB 2015. The mechanism of small peptide absorption in bovine omasal epithelial cells and charactrization of bovine peptide transporter 1. PhD thesis, Zhejiang University, Hangzhou, China.
Zhang, AH 2014. Ontogenetic regulation of nutrient transporters in the small intestine of broilers. Master thesis, Chinese Academy of Agricultural Science Dissertation, Beijing, China.
Zhang, S, Qiao, S, Ren, M, Zeng, X, Ma, X, Wu, Z, Thacker, P and Wu, G 2013. Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs. Amino Acids 45, 11911205.
Zhou, SG, Feng, DY, Huang, ZY, Zuo, JJ, Zhi, AM, Dong, ZM, Zhang, CM and Ye, H 2009. Segmental distribution and ontogenetic regulation of peptide transporter 1(PepT1) mRNA expression in intestine of pigs. Journal of Agricultural Biotechnology 17, 229236.


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Rabbit SLC15A1, SLC7A1 and SLC1A1 genes are affected by site of digestion, stage of development and dietary protein content

  • L. Liu (a1), H. Liu (a1), L. Ning (a1) and F. Li (a1)


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