Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-25T15:59:14.269Z Has data issue: false hasContentIssue false
  • English
  • Français

Probiotics in milk replacer influence lamb immune function and meat quality

Published online by Cambridge University Press:  19 September 2011

A. Santillo ,
G. Annicchiarico ,
M. Caroprese ,
R. Marino ,
A. Sevi  and
M. Albenzio
A. Santillo
Affiliation:
Department of Production Sciences and Innovation in Mediterranean Agriculture and Food Systems (PrIME), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy
G. Annicchiarico
Affiliation:
Agricultural and Research Council (CRA-ZOE), Via Napoli, 71020 Segezia-Foggia, Italy
M. Caroprese
Affiliation:
Department of Production Sciences and Innovation in Mediterranean Agriculture and Food Systems (PrIME), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy
R. Marino
Affiliation:
Department of Production Sciences and Innovation in Mediterranean Agriculture and Food Systems (PrIME), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy
A. Sevi
Affiliation:
Department of Production Sciences and Innovation in Mediterranean Agriculture and Food Systems (PrIME), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy
M. Albenzio*
Affiliation:
Department of Production Sciences and Innovation in Mediterranean Agriculture and Food Systems (PrIME), University of Foggia, Via Napoli, 25, 71100 Foggia, Italy
*
†E-mail: m.albenzio@unifg.it
Get access

Abstract

This study was undertaken to assess the effect of milk replacer (MR) containing Lactobacillus acidophilus and a mix of Bifidobacterium animalis subsp. lactis and Bifidobacterium longum subsp. longum on lamb immune response and on lamb meat quality. A 6-week-trial was conducted on 40 male Comisana lambs, divided into four groups, fed maternal milk (MM), MR, MR with L. acidophilus supplementation (MRL) and MR with a mix (1 : 1) of B. animalis subsp. lactis and B. longum subsp. longum supplementations (MRB). Lambs fed MR containing a mix of bifidobacteria showed the highest in vivo cellular immune response to phytohemagglutinin, whereas MM and MRB showed the highest antibody response to ovalbumin. At day 11 of the trial, MRL displayed the highest value of Interleukin-10; differences disappeared among groups subsequently. Blood cholesterol levels in lambs fed MR containing L. acidophilus was almost halved compared with that found in MM and MR groups. Meat from artificially reared lambs was characterized by trans-11 18:1 and total conjugated 18:2n-6, whereas meat from the dam-suckled lambs was characterized by 14:0, cis-9 14:1 and 16:0. Polyunsaturated to saturated fatty acid ratio was higher in meat of MR, MRL and MRB than in MM lambs. Meat from artificially reared lamb fed MR containing probiotics showed an improved fatty acid profile for human diet.

Keywords

lambprobioticimmune functionmeat qualityfatty acids
Type
Full Paper
Information
animal , Volume 6 , Issue 2 , 03 January 2012 , pp. 339 - 345
Copyright
Copyright © The Animal Consortium 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agarwal, N, Kamra, DN, Chaudhary, LC, Agarwal, I, Sahoo, A, Pathak, NN 2002. Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives. Letters in Applied Microbiology 34, 329–336.CrossRefGoogle ScholarPubMed
Alonso, L, Cuesta, EP, Gilliland, SE 2003. Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. Journal of Dairy Science 86, 1941–1946.CrossRefGoogle ScholarPubMed
Anadón, A, Martínez-Larrañaga, MR, Aranzazu Martínez, M 2006. Probiotics for animal nutrition in the European Union. Regulation and safety assessment. Regulatory Toxicology and Pharmacology 45, 91–95.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists 1990. Official methods of analysis Vol. 1, 15th edition. AOAC, Arlington, VA, USA.Google Scholar
Association of Official Analytical Chemists 1995. Official methods of analysis Vol. 11, 16th edition. AOAC, Arlington, VA, USA.Google Scholar
Bligh, EG, Dyer, WJ 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemical Physiology 37, 911.CrossRefGoogle ScholarPubMed
Caroprese, M, Albenzio, M, Annicchiarico, G, Sevi, A 2006. Changes occurring in immune responsiveness of single- and twin-bearing Comisana ewes during the transition period. Journal of Dairy Science 89, 562–568.CrossRefGoogle ScholarPubMed
Caroprese, M, Marzano, A, Entrican, G, Wattegeder, S, Albenzio, M, Sevi, A 2009. Immune response of cows fed polyunsaturated fatty acids under high ambient temperatures. Journal of Dairy Science 92, 2796–2803.CrossRefGoogle ScholarPubMed
Coakley, M, Ross, RP, Nordgren, M, Fitzgerald, G, Devery, R, Stanton, C 2003. Conjugated linoleic acid biosynthesis by human-derived Bifidobacterium species. Journal of Applied Microbiology 94, 138–145.CrossRefGoogle ScholarPubMed
Cruywagen, CW, Jordaan, I, Venter, L 1996. Effect of Lactobacillus acidophilus supplementation of milk replacer on preweaning performance of calves. Journal of Dairy Science 79, 483–486.CrossRefGoogle ScholarPubMed
Delavaud, C, Bocquier, F, Chilliard, Y, Keisler, DH, Gertler, A, Kann, G 2000. Plasma leptin determination in ruminants: effect of nutritional status and body fatness on plasma leptin concentration assessed by a specific RIA in sheep. Journal of Endocrinology 165, 519–526.CrossRefGoogle ScholarPubMed
European Communities 1986. Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations, and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes Off. J. L 358. European Communities Publication Office, Luxembourg, pp. 1–28.Google Scholar
Folch, J, Lees, M, Stanley, SGH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Galdeano, CM, Perdigón, G 2004. Role of viability of probiotic strains in their persistence in the gut and in mucosal immune stimulation. Journal of Applied Microbiology 97, 673–681.CrossRefGoogle ScholarPubMed
Gill, HS, Cross, ML 2002. Probiotics and immune function. In Nutrition and immune function (ed. PC Calder, CJ Field and HS Gill), pp. 251–272. CABI Publishing, Wallingford, UK.CrossRefGoogle Scholar
Lema, M, Williams, L, Rao, DR 2001. Reduction of fecal shedding of enterohemorrhagic Escherichia coli O157:H7 in lambs by feeding microbial feed supplement. Small Ruminant Research 39, 31–39.CrossRefGoogle ScholarPubMed
Lubbadeh, W, Haddadin, MSY, Al-Tamimi, MA, Robinson, RK 1999. Effect on the cholesterol content of fresh lamb of supplementing the feed of Awassi ewes and lambs with Lactobacillus acidophilus. Meat Science 52, 381–385.CrossRefGoogle ScholarPubMed
Lys, MB, Gilliland, SE 1994. Comparisons of freshly isolated strains of L. acidophilus of human intestinal origin for ability to assimilate cholesterol during growth. Journal of Dairy Science 77, 2925–2933.Google Scholar
Kwong, LS, Hope, JC, Thom, ML, Sopp, P, Duggan, S, Bembridge, GP, Howard, CJ 2002. Development of an ELISA for bovine IL-10. Veterinary Immunology Immunopathology 85, 213–223.CrossRefGoogle ScholarPubMed
Maiorano, G, Ciarlariello, A, Cianciullo, D, Roychoudhury, S, Manchisi, A 2009. Effect of suckling management on productive performance, carcass traits and meat quality of Comisana lambs. Meat Science 83, 577–583.CrossRefGoogle ScholarPubMed
Marino, R, Albenzio, M, Annicchiarico, G, Caroprese, M, Muscio, A, Santillo, A, Sevi, A 2008. Influence of genotype and slaughtering age on meat from Altamurana and Trimeticcio lambs. Small Ruminant Research 78, 144–151.CrossRefGoogle Scholar
Napolitano, F, Marino, V, De Rosa, G, Capparelli, R, Bordi, A 1995. Influence of artificial rearing on behavioural and immune response of lambs. Applied Animal Behaviour Science 45, 245–253.CrossRefGoogle Scholar
Napolitano, F, Cifuni, GF, Pacelli, C, Riviezzi, AM, Girolami, A 2002. Effect of artificial rearing on lamb welfare and meat quality. Meat Science 60, 307–315.CrossRefGoogle ScholarPubMed
Napolitano, F, Caroprese, M, Girolami, A, Marino, R, Muscio, A, Sevi, A 2006. Effects of early maternal separation of lambs and rearing with minimal and maximal human contact on meat quality. Meat Science 72, 635–640.CrossRefGoogle ScholarPubMed
Niers, LE, Timmerman, HM, Rijkers, GT, Van Bleek, GM, Van Uden, NO, Knol, EFM, Kapsenberg, L, Kimpen, JL, Hoekstra, MO 2005. Identification of strong interleukin-10 inducing lactic acid bacteria which down-regulate T helper type 2 cytokines. Clinical and Experimental Allergy 35, 1481–1489.CrossRefGoogle ScholarPubMed
Oriani, G, Maiorano, G, Filetti, F, Di Cesare, C, Manchisi, A, Salvatori, G 2005. Effect of age on fatty acid composition of Italian Merino suckling lambs. Meat Science 71, 557–562.CrossRefGoogle ScholarPubMed
Pereira, DIA, Gibson, GR 2002. Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Applied and Environmental Microbiology 68, 4689–4693.CrossRefGoogle ScholarPubMed
Sauter, SN, Allenspach, K, Gaschen, F, Gröne, A, Ontsouka, E, Blum, JW 2005. Cytokine espression in an ex vivo culture system of duodenal samples from dogs with chronic enteropathies: modulation by probiotic bacteria. Domestic Animal Endocrinology 29, 605–622.CrossRefGoogle Scholar
Scharek, L, Guth, J, Reiter, K, Weyrauch K, D, Taras, D, Schwerk, P, Schierack, P, Schmidt, MFG, Wieler, LH, Tedin, K 2005. Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets. Veterinary Immunology and Immunopathology 105, 151–161.CrossRefGoogle ScholarPubMed
Schmid, A, Collomb, M, Sieber, R, Bee, G 2006. Conjugated linoleic acid in meat and meat products: a review. Meat Science 73, 29–41.CrossRefGoogle ScholarPubMed
Sevi, A, Napolitano, F, Casamassima, D, Annicchiarico, G, Quarantelli, T, De Paola, R 1999. Effect of gradual transition from maternal to reconstituted milk on behavioural, endocrine and immune responses of lambs. Applied Animal Behavior Science 64, 249–259.CrossRefGoogle Scholar
Sevi, A, Caroprese, M, Annicchiarico, G, Albenzio, M, Taibi, L, Muscio, A 2003. The effect of a gradual separation from the mother on later behavioral, immune and endocrine alterations in artificially reared lambs. Applied Animal Behavior Science 83, 41–53.CrossRefGoogle Scholar
Shapiro, SS, Wilk, M 1965. An analysis of variance test for normality. Biometrika 52, 591–601.CrossRefGoogle Scholar
Sherman, MA, Kalman, D 2004. Initiation and resolution of mucosal inflammation. Immunology Research 29, 241–252.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute 1999. Statistical analysis systems, version 8.1. SAS Institute Inc., Cary, NC.Google Scholar
Velasco, S, Caneque, V, Perez, C, Lauzurica, S, Diaz, MT, Huidobro, F, Manzanares, C, Gonzalez, J 2001. Fatty acid composition of adipose depots of suckling lambs raised under different production system. Meat Science 59, 325–333.CrossRefGoogle Scholar
Zygoyiannis, D, Kufidis, D, Katsaounis, N, Phillips, P 1992. Fatty acid composition of carcass fat of indigenous (Capra priscu) suckled Greek kids and milk of their does. Small Ruminant Research 8, 83–95.CrossRefGoogle Scholar