Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-18T14:44:42.541Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization, polymorphism and association study of lysozyme gene with milk production and somatic cell trait in Bos indicus × Bos taurus cattle

Published online by Cambridge University Press:  01 May 2009

M. Salehin ,
A. K. Ghosh ,
P. K. Mallick  and
T. K. Bhattacharya
M. Salehin
Affiliation:
Department of Animal Genetics and Breeding, Veterinary College, GBPUA&T, Pantnagar, Uttaranchal, India
A. K. Ghosh
Affiliation:
Department of Animal Genetics and Breeding, Veterinary College, GBPUA&T, Pantnagar, Uttaranchal, India
P. K. Mallick
Affiliation:
Department of Animal Genetics and Breeding, Veterinary College, GBPUA&T, Pantnagar, Uttaranchal, India
T. K. Bhattacharya*
Affiliation:
Project Directorate on Poultry, Rajendranagar, Hyderabad, Andhra Pradesh 500030, India
*
E-mail: bhattacharyatk@gmail.com
Get access

Abstract

Lysozyme, an anti-bacterial enzyme, is mostly found in the body fluids, various tissues and secretions of animals and humans, and confers immunity against a wide range of bacterial species. The present study was carried out to elucidate the gene sequence of this enzyme in Indian Sahiwal × Holstein Friesian crossbred cattle and to explore the polymorphism of the gene as well as their association with milk production and somatic cell traits. The total length of lysozyme cDNA was found to be of 447 bp. The similarity with Bos taurus, human, pig, monkey, gorilla, mice, rat, chicken, dog and sheep was estimated as 99.1%, 85%, 81.0%, 85.2%, 84.3%, 77.9%, 77.9%, 41.4%, 40.5% and 24.6%, respectively. Polymorphism study of two fragments, extended exon1 including promoter, exon1 and partial intron1 (268 bp), and extended exon2 including partial intron1 and 2, and exon2 region (287 bp) of milk lysozyme gene was carried out by employing single-stranded conformation polymorphism (SSCP). In the extended exon1 fragment, three alleles namely A, B and C with frequencies of 0.59, 0.28 and 0.12 were observed while in the exon extended fragment, three different alleles – P, Q and R with respective frequencies of 0.61, 0.38 and 0.01 – were determined in Indian Sahiwal × Holstein Friesian cross. A total of eight haplotypes were found in this population where the most predominant one was h1 (0.52). Genotypes of exon1 extended fragment showed significant association with total milk yield, daily milk yield, peak yield and somatic cell score at P < 0.05 while that of exon2 extended fragment had significant correlation with only total lactational milk yield. Haplotype combinations also revealed significant association with total milk production where h1h1 homozygous showed highest yield during first lactation.

Keywords

associationcattlehaplotypemilk lysozyme genepolymorphism
Type
Full Paper
Information
animal , Volume 3 , Issue 5 , May 2009 , pp. 623 - 631
Copyright
Copyright © The Animal Consortium 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Badola, S, Bhattacharya, TK, Biswas, TK, Kumar, P, Sharma, A 2003. Association of beta-lactoglobulin polymorphism with milk production traits in cattle. Asian–Australasian Journal of Animal Sciences 16, 1560–1564.CrossRefGoogle Scholar
Chandan, RC, Shahani, K, Holly, RG 1964. Lysozyme content of human milk. Nature 204, 76–77.CrossRefGoogle ScholarPubMed
Falconer, DS, Mackay, TFC 1996. Introduction to quantitative genetics, 4th edition. Addison-Wesley Longman Ltd, Essex, England.Google Scholar
Goudswaard, J, Bakker-de Koff, EC, Van Ravenswaaij-Kraam, HP 1978. Lysozyme and its presence in bovine milk and serum. Tijdschrift voor diergeneeskunde 103, 445–450.Google ScholarPubMed
Grün, E 1985. The physiological and diagnostic importance of lysozyme in cow’s milk. Allergie und Immunologie 31, 3–15.Google ScholarPubMed
Harvey, WR 1990. User’s guide for LSMLMW, mixed model least squares and maximum likelihood computer program. Ohio State University, Columbus, OH (Mimeograph).Google Scholar
Jeanloz, RW, Sharon, N, Flowers, HM 1963. The chemical structure of a disaccharide isolated from Micrococcus lysodeikticus cell wall. Biochemical and Biophysical Research Communications 13, 20–25.CrossRefGoogle ScholarPubMed
Nguyen-Huu, MC, Stratmann, M, Groner, B, Wurtz, T, Land, H, Giesecke, K, Sippel, AE, Schutz, G 1979. Chicken lysozyme gene contains several intervening sequences. Proceedings of the National Academy of Sciences of the United States of America 76, 76–80.CrossRefGoogle ScholarPubMed
Rost, B, Sander, C 1993. Prediction of protein secondary structure at better than 70% accuracy. Journal of Molecular Biology 232, 584–599.CrossRefGoogle ScholarPubMed
Rost, B, Fariselli, P, Casadio, R 1996. Topology prediction for helical transmembrane proteins at 86% accuracy. Protein Science 5, 1704–1718.CrossRefGoogle ScholarPubMed
Sambrook, J, Russell, DW 2001. Molecular cloning: a laboratory manual, 3rd edition, vols. 1 to 3 . Cold Spring Harbor Laboratory Press, New York, USA.Google Scholar
Schmedt Auf Der Günne, H, Tenhagen, BA, Kutzer, P, Forderung, D, Heuwieser, W 2002. Do lactoferrin, lysozyme and the lactoperoxidase–thiocyanate–hydrogen peroxide system cause negative microbiological results in mastitis secretions. Deutsche tierärztliche Wochenschrift 109, 300–305.Google ScholarPubMed
Sharma, A, Dhingra, P, Pander, BL, Kumar, R 2006. Bovine subclinical mastitis: prevalence and treatment with homeopathic medicine. International Journal of Cow Science 2, 40–44.Google Scholar
Shashikanth, G, Yadav, BR 2005. DNA polymorphisms in the Sahiwal breed of Zebu cattle revealed by synthetic oligonucleotide probes. In Applications of gene-based technologies for improving animal production and health in developing countries (ed. HPS Makkar and GJ Viljoen), pp. 111–120. Springer, Dordrecht, The Netherlands.CrossRefGoogle Scholar
Sigurdardottir, S, Lunden, A, Anderson, L 1990. Restriction fragment length polymorphism of bovine lysozyme genes. Animal Genetics 21, 259–265.CrossRefGoogle ScholarPubMed
Sordillo, LM, Shafer-Weaver, K, DeRosa, D 1997. Immunobiology of the mammary gland. Journal of Dairy Science 80, 1851–1865.CrossRefGoogle ScholarPubMed
Vohra, V, Bhattacharya, TK, Kumar, P, Sharma, A 2006. Genetic variants of beta-lactoglobulin gene and its association with milk composition traits in riverine buffalo. Journal of Dairy Research 73, 499–503.CrossRefGoogle ScholarPubMed