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Impacts of protein supplementation during late gestation of beef cows on maternal skeletal muscle and liver tissues metabolism

Published online by Cambridge University Press:  16 March 2020

R. C. Lopes ,
C. B. Sampaio ,
A. S. Trece ,
P. D. Teixeira ,
T. R. S. Gionbelli ,
L. R. Santos ,
T. C. Costa ,
M. S. Duarte  and
M. P. Gionbelli Open the ORCID record for M. P. Gionbelli [Opens in a new window]
R. C. Lopes
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil Trouw Nutrition, Mirassol, SP15130-000, Brazil
C. B. Sampaio
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
A. S. Trece
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
P. D. Teixeira
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
T. R. S. Gionbelli
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
L. R. Santos
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
T. C. Costa
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
M. S. Duarte
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
M. P. Gionbelli*
Affiliation:
Department of Animal Science, Universidade Federal de Lavras, Lavras, MG37200-900, Brazil
*
†E-mail: mateus.pg@ufla.br
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Abstract

Since nutritional requirements are increased at the end of gestation to meet the demands of the pregnant uterus, pregnant beef cows are susceptible to mobilization of body reserves (mainly fat and amino acids (AAs)) and to alter the metabolism of nutrients in the liver and muscle to support such demands. The objective of this study was to evaluate the effect of CP supplementation on maternal nutrient metabolism in the late gestation of beef cows grazing a low-quality pasture. Forty-three pregnant Nellore cows gestating male fetuses (average age = 6 years; average weight = 544 kg) at 193 ± 30 (mean ± SD) days (d) of gestation were divided into eight groups (experimental units, with four to five cows each). Treatments were (1) control (CON, n = 4): pasture-based (PB) diet without CP supplementation and (2) supplemented (SUP, n = 4): PB diet daily supplemented with 2 g/kg of BW of a 43.5% CP supplement. Liver and skeletal muscle biopsies were performed at 265 days of gestation and samples were collected for mRNA expression. On day 280 of gestation, blood samples were collected to assess plasma levels of AA. The CON-fed cows tended to have greater (P = 0.057) total circulating AA than SUP-fed cows. The circulating glycogenic AA was greater (P = 0.035) in CON than in SUP cows. CON cows was greater for histidine (P = 0.015), methionine (P = 0.007) and alanine (P = 0.036) than SUP cows. The CON- and SUP-fed showed no differences for gluconeogenesis, fatty acid transport and signaling axis markers in the liver. The mRNA expression of markers for skeletal muscle synthesis, p7056k (P = 0.060) and GSK3B (P = 0.096), tended to be greater in cows from CON than SUP group. No differences were found for mRNA expression of markers for skeletal muscle degradation. We conclude that CP supplementation to CP-restricted late-pregnant beef cows reduces the maternal tissue mobilization and changes the profile of plasma circulating AA and the mRNA expression of markers for the synthesis of skeletal muscle tissue.

Keywords

amino acidsfetal programminggluconeogenesismaternal nutritiontissue mobilization
Type
Research Article
Information
animal , Volume 14 , Issue 9 , September 2020 , pp. 1867 - 1875
Copyright
© The Animal Consortium 2020

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