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High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats

  • R. Y. Zhang (a1), W. Jin (a2), P. F. Feng (a2), J. H. Liu (a2) and S. Y. Mao (a2)...


In the current intensive production system, ruminants are often fed high-grain (HG) diets. However, this feeding pattern often causes rumen metabolic disorders and may further trigger laminitis, the exact mechanism is not clear. This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue. In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6). On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected. The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood. HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered. HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group. Correlation analysis indicated that the expression of pro-inflammatory cytokines were positively correlated with MMP-2 expression in lamellar tissues. Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.


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Bassi, DE, Lopez De Cicco, R, Cenna, J, Litwin, S, Cukierman, E and Klein-Szanto, AJ 2005. PACE4 expression in mouse basal keratinocytes results in basement membrane disruption and acceleration of tumor progression. Cancer Research 65, 73107319.
Bicalho, RC, Machado, VS and Caixeta, LS 2009. Lameness in dairy cattle: a debilitating disease or a disease of debilitated cattle? A cross-sectional study of lameness prevalence and thickness of the digital cushion. Journal of Dairy Science 92, 31753184.
Clutterbuck, AL, Harris, P, Allaway, D and Mobasheri, A 2010. Matrix metalloproteinases in inflammatory pathologies of the horse. Veterinary Journal 183, 2738.
Cotta, MA 1988. Amylolytic activity of selected species of ruminal bacterial. Applied and Environmental Microbiology 54, 772776.
Emmanuel, DG, Dunn, SM and Ametaj, BN 2008. Feeding high proportions of barley grain stimulates an inflammatory response in dairy cows. Journal of Dairy Science 91, 606614.
Fernando, SC, Purvis, HT, Najar, FZ, Sukharnikov, LO, Krehbiel, CR, Nagaraja, TG, Roe, BA and Desilva, U 2010. Rumen microbial population dynamics during adaptation to a high-grain diet. Applied and Environmental Microbiology 76, 74827490.
Greenough, PR, Vermunt, JJ, McKinnon, JJ, Fathy, FA, Berg, PA and Cohen, RD 1990. Laminitis-like changes in the claws of feedlot cattle. Canadian Veterinary Journal 31, 202208.
Hendry, KA, Knight, CH, Galbraith, H and Wilde, CJ 2003. Basement membrane integrity and keratinization in healthy and ulcerated bovine hoof tissue. Journal of Dairy Research 70, 1927.
Hua, C, Tian, J, Tian, P, Cong, R, Luo, Y, Geng, Y, Tao, S, Ni, Y and Zhao, R 2017. Feeding a high concentration diet induces unhealthy alterations in the composition and metabolism of ruminal microbiota and host response in a goat model. Frontiers in Microbiology 8, 138.
Johnson, PJ, Kreeger, JM, Keeler, M, Ganjam, VK and Messer, NT 2000. Serum markers of lamellar basement membrane degradation and lamellar histopathological changes in horses affected with laminitis. Equine Veterinary Journal 32, 462468.
Khafipour, E, Krause, DO and Plaizier, JC 2009a. A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. Journal of Dairy Science 92, 10601070.
Khafipour, E, Li, S, Plaizier, JC and Krause, DO 2009b. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Applied and Environmental Microbiology 75, 71157124.
Khafipour, E, Li, S, Tun, HM, Derakshani, H, Moossavi, S and Plaizier, JC 2016. Effects of grain feeding on microbiota in the digestive tract of cattle. Animal Frontiers 6, 1319.
Klieve, AV, O’Leary, MN, McMillen, L and Ouwerkerk, D 2007. Ruminococcus bromii, identification and isolation as a dominant community member in the rumen of cattle fed a barley diet. Journal of Applied Microbiology 103, 20652073.
Kossakowska, AE, Edwards, DR, Prusinkiewicz, C, Zhang, MC, Guo, D, Urbanski, SJ, Grogan, T, Marquez, LA and Janowska-Wieczorek, A 1999. Interleukin-6 regulation of matrix metalloproteinase (MMP-2 and MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) expression in malignant non-Hodgkin’s lymphomas. Blood 94, 20802089.
Kyaw-Tanner, M and Pollitt, CC 2004. Equine laminitis: increased transcription of matrix metalloproteinase-2 (MMP-2) occurs during the developmental phase. Equine Veterinary Journal 36, 221225.
Leise, BS, Faleiros, RR, Watts, M, Johnson, PJ, Black, SJ and Belknap, JK 2011. Laminar inflammatory gene expression in the carbohydrate overload model of equine laminitis. Equine Veterinary Journal 43, 5461.
Li, XR, Jiang, RL, Wang, GY, Li, Y, Fan, XJ, Liu, X, Wang, JL, Pan, JL and Gao, L 2015. MMP-2 plays an important role during the early acute developmental phase of oligofructose-induced equine laminitis. Bulletin of the Veterinary Institute in Pulawy 59, 149153.
Liu, JH, Xu, TT, Liu, YJ, Zhu, WY and Mao, SY 2013. A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats. American Journal of Physiology – Regulatory Integrative and Comparative Physiology 305, R232R241.
Loftus, JP, Johnson, PJ, Belknap, JK, Pettigrew, A and Black, SJ 2009. Leukocyte-derived and endogenous matrix metalloproteinases in the lamellae of horses with naturally acquired and experimentally induced laminitis. Veterinary Immunology and Immunopathology 129, 221230.
Manichanh, C, Rigottier-Gois, L, Bonnaud, E, Gloux, K, Pelletier, E, Frangeul, L, Nalin, R, Jarrin, C, Chardon, P, Marteau, P, Roca, J and Dore, J 2006. Reduced diversity of faecal microbiota in Crohn’s disease revealed by a metagenomic approach. Gut 55, 205211.
Nocek, JE 1997. Bovine acidosis: implications on laminitis. Journal of Dairy Science 80, 10051028.
Nourian, AR, Asplin, KE, McGowan, CM, Sillence, MN and Pollitt, CC 2009. Equine laminitis: ultrastructural lesions detected in ponies following hyperinsulinaemia. Equine Veterinary Journal 41, 671677.
Owens, F, Secrist, D, Hill, W and Gill, D 1998. Acidosis in cattle: a review. Journal of Animal Science 76, 275286.
Plaizier, JC, Khafipour, E, Li, S, Gozho, GN and Krause, DO 2012. Subacute ruminal acidosis (SARA), endotoxins and health consequences. Animal Feed Science and Technology 172, 921.
Plaizier, JC, Li, S, Tun, HM and Khafipour, E 2016. Nutritional models of experimentally-induced subacute ruminal acidosis (SARA) differ in their impact on rumen and hindgut bacterial communities in dairy cows. Frontiers in Microbiology 7, 2128.
Pollitt, CC 1996. Basement membrane pathology: a feature of acute equine laminitis. Equine Veterinary Journal 28, 3846.
Saleem, F, Ametaj, BN, Bouatra, S, Mandal, R, Zebeli, Q, Dunn, SM and Wishart, DS 2012. A metabolomics approach to uncover the effects of grain diets on rumen health in dairy cows. Journal of Dairy Science 95, 66066623.
Sato, H, Takino, T, Kinoshita, T, Imai, K, Okada, Y, Stetler Stevenson, WG and Seiki, M 1996. Cell surface binding and activation of gelatinase A induced by expression of membrane-type-1-matrix metalloproteinase (MT1-MMP). FEBS Letters 385, 238240.
Steele, MA, Croom, J, Kahler, M, AlZahal, O, Hook, SE, Plaizier, K and McBride, BW 2011. Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis. American Journal of Physiology-regulatory Integrative and Comparative Physiology 300, R1515R1523.
Stevenson, DM and Weimer, PJ 2007. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Applied Microbiology and Biotechnology 75, 165174.
van Eps, AW and Pollitt, CC 2004. Equine laminitis: cryotherapy reduces the severity of the acute lesion. Equine Veterinary Journal 36, 255260.
Webster, AJ, Knott, L and Tarlton, JF 2005. Understanding lameness in the dairy cow. Cattle Practice 13, 9398.
Zhang, RY, Ye, HM, Liu, JH and Mao, SY 2017. High-grain diets altered rumen fermentation and epithelial bacterial community and resulted in rumen epithelial injuries of goats. Applied Microbiology and Biotechnology 101, 69816992.


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