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High concentrate-induced subacute ruminal acidosis (SARA) increases plasma acute phase proteins (APPs) and cortisol in goats

  • Y. Y. Jia (a1), S. Q. Wang (a1), Y. D. Ni (a1), Y. S. Zhang (a1), S. Zhuang (a1) and X. Z. Shen (a1)...

Abstract

The aim of this study was to investigate changes of stress status in dairy goats induced to subacute ruminal acidosis (SARA). The level of acute phase proteins (APPs) including haptoglobin (HP) and serum amyloid A (SAA) in plasma and their mRNA expression in liver, as well as plasma cortisol and genes expression of key factors controlling cortisol synthesis in adrenal cortex were compared between SARA and control goats. SARA was induced by feeding high concentrate diet (60% concentrate of dry matter) for 3 weeks (SARA, n=6), while control goats (Con, n=6) received a low concentrate diet (40% concentrate of dry matter) during the experimental time. SARA goats showed ruminal pH below 5.8 for more than 3 h per day, which was significantly lower than control goats (pH>6.0). SARA goats demonstrated a significant increase of hepatic HP and SAA mRNA expression (P<0.05), and the level of HP but not SAA in plasma was markedly increased compared with control (P<0.05). The level of cortisol in plasma showed a trend to increase in SARA goats (0.05<P<0.1). In adrenal cortex, mRNA expression of 17α-hydroxylase cytochrome (P45017α ) (P<0.01) and 3β-hydroxysteroid dehydrogenase (3β-HSD) (P<0.05) was significantly increased in SARA goats. The contents of 3β-HSD and P450 side-chain cleavage protein were increased by 58.6% and 39.4%, respectively, but did not reach the statistical significance (P>0.05). These results suggested that SARA goats experienced a certain stress status, exhibiting an increase in HP production and cortisol secretion.

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Baumann, H and Gauldie, J 1994. The acute phase response. Immunol Today 15, 7480.
Beauchemin, K, Yang, W and Rode, L 2003. Effects of particle size of alfalfa-based dairy cow diets on chewing activity, ruminal fermentation, and milk production. Journal of Dairy Science 86, 630643.
Bode, JG, Albrecht, U, Häussinger, D, Heinrich, PC and Schaper, F 2012. Hepatic acute phase proteins – regulation by IL-6- and IL-1-type cytokines involving STAT3 and its crosstalk with NF-κB-dependent signaling. European Journal of Cell Biology 91, 496505.
Desnoyers, M, Duvaux-Ponter, C, Rigalma, K, Roussel, S, Martin, O and Giger-Reverdin, S 2008. Effect of concentrate percentage on ruminal pH and time-budget in dairy goats. Animal 2, 18021808.
Elam, C 1976. Acidosis in feedlot cattle: practical observations. Journal of Animal Science 43, 898901.
Enemark, JMD, Jørgensen, RJ and Kristensen, NB 2004. An evaluation of parameters for the detection of subclinical rumen acidosis in dairy herds. Veterinary Research Communications 28, 687709.
González, FH, Ruipérez, FH, Sánchez, JM, Souza, JC, Martínez-Subiela, S and Cerón, JJ 2010. Haptoglobin and serum amyloid A in subacute ruminal acidosis in goats. Revista de la Facultad de Medicina Veterinaria y de Zootecnia 57, 159167.
Gozho, GN, Krause, DO and Plaizier, JC 2006. Rumen lipopolysaccharide and inflammation during grain adaptation and subacute ruminal acidosis in steers. Journal of Dairy Science 89, 44044413.
Gozho, GN, Krause, DO and Plaizier, JC 2007. Ruminal lipopolysaccharide concentration and inflammatory response during grain-induced subacute ruminal acidosis in dairy cows. Journal of Dairy Science 90, 856866.
Gozho, G, Plaizier, J, Krause, D, Kennedy, A and Wittenberg, K 2005. Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response. Journal of Dairy Science 88, 13991403.
Hall, P 1986. Cytochromes p-450 and the regulation of steroid synthesis. Steroids 48, 131196.
Huang, HS, Wu, MC and Li, PH 2000. Expression of steroidogenic enzyme messenger ribonucleic acid and cortisol production in adrenocortical cells isolated from halothane-sensitive and halothane-resistant pigs. Journal of Cellular Biochemistry 79, 5870.
Huntington, G 1990. Energy metabolism in the digestive tract and liver of cattle: influence of physiological state and nutrition. Reproduction, Nutrition, Development 30, 3547.
Iqbal, S, Zebeli, Q, Mazzolari, A, Dunn, S and Ametaj, B 2010. Feeding rolled barley grain steeped in lactic acid modulated energy status and innate immunity in dairy cows. Journal of Dairy Science 93, 51475156.
Jarvis, S, Moinard, C, Robson, SK, Baxter, E, Ormandy, E, Douglas, AJ, Seckl, JR, Russell, JA and Lawrence, AB 2006. Programming the offspring of the pig by prenatal social stress: neuroendocrine activity and behaviour. Hormones and Behavior 49, 6880.
Kaminska, B, Ciereszko, R, Kiezun, M and Dusza, L 2013. In vitro effects of genistein and daidzein on the activity of adrenocortical steroidogenic enzymes in mature female pigs. Journal of Physiology and Pharmacology 64, 103108.
Karrow, NA, You, Q, McNicoll, C and Hay, J 2010. Activation of the ovine hypothalamic-pituitary-adrenal axis and febrile response by interleukin-6: a comparative study with bacterial lipopolysaccharide endotoxin. Canadian Journal of Veterinary Research 74, 3033.
Khafipour, E, Krause, D and Plaizier, J 2009. A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. Journal of Dairy Science 92, 10601070.
Kleen, J, Hooijer, G, Rehage, J and Noordhuizen, J 2003. Subacute ruminal acidosis (sara): a review. Journal of Veterinary Medicine Series A 50, 406414.
Klevenhusen, F, Hollmann, M, Podstatzky-Lichtenstein, L, Krametter-Frötscher, R, Aschenbach, JR and Zebeli, Q 2013. Feeding barley grain-rich diets altered electrophysiological properties and permeability of theruminal wall in a goat model. Journal of Dairy Science 96, 22932302.
Krause, K and Oetzel, G 2005. Inducing subacute ruminal acidosis in lactating dairy cows. Journal of Dairy Science 88, 36333639.
Kristina, F, Örjan, L and Bernt, J 2010. Low cortisol levels in blood from dairy cows with ketosis: a field study. Acta Veterinaria Scandinavica 52, 3136.
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25, 402408.
Miller, WL 1988. Molecular biology of steroid hormone synthesis. Endocrine Reviews 9, 295318.
Mudron, P, Scholz, H, Sallmann, H, Rehage, J, Kovac, G, Bartko, F and Höltershinken, M 1994. Effect of vitamin e injection on cortisol and white blood cell response to surgical stress in dairy cows. International Journal for Vitamin and Nutrition Research/Internationale Zeitschrift für Vitamin-und Ernährungsforschung. Journal International de Vitaminologie et de Nutrition 64, 176180.
Murata, H, Shimada, N and Yoshioka, M 2004. Current research on acute phase proteins in veterinary diagnosis: an overview. The Veterinary Journal 168, 2840.
Plaizier, J, Krause, D, Gozho, G and McBride, B 2008. Subacute ruminal acidosis in dairy cows: the physiological causes, incidence and consequences. The Veterinary Journal 176, 2131.
Rutherford, K, Haskell, MJ, Glasbey, C and Lawrence, AB 2006. The responses of growing pigs to a chronic-intermittent stress treatment. Physiology & Behavior 89, 670680.
Sauvant, D, Meschy, F and Mertens, D 1999. Les composantes de l’acidose ruminale et les effets acidogenes des rations. INRA Productions Animales 12, 4960.
Steiger, M, Senn, M, Altreuther, G, Werling, D, Sutter, F, Kreuzer, M and Langhans, W 1999. Effect of a prolonged low-dose lipopolysaccharide infusion on feed intake and metabolism in heifers. Journal of Animal Science 77, 25232532.
Stocco, DM and Clark, BJ 1996. Regulation of the acute production of steroids in steroidogenic cells. Endocrine Reviews 17, 221244.
Sugawara, T, Holt, JA, Driscoll, D, Strauss, J, Lin, D, Miller, WL, Patterson, D, Clancy, KP, Hart, IM and Clark, BJ 1995. Human steroidogenic acute regulatory protein: functional activity in cos-1 cells, tissue-specific expression, and mapping of the structural gene to 8p11. 2 and a pseudogene to chromosome 13. Proceedings of the National Academy of Sciences 92, 47784782.
Thüer, S, Mellema, S, Doherr, MG, Wechsler, B, Nuss, K and Steiner, A 2007. Effect of local anaesthesia on short-and long-term pain induced by two bloodless castration methods in calves. The Veterinary Journal 173, 333342.
Van Metre, DC, Tyler, JW and Stehman, SM 2000. Diagnosis of enteric disease in small ruminants. Veterinary Clinics of North America: Food Animal Practice 16, 87115.
Waldron, M, Nishida, T, Nonnecke, B and Overton, T 2003. Effect of lipopolysaccharide on indices of peripheral and hepatic metabolism in lactating cows. Journal of Dairy Science 86, 34473459.

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High concentrate-induced subacute ruminal acidosis (SARA) increases plasma acute phase proteins (APPs) and cortisol in goats

  • Y. Y. Jia (a1), S. Q. Wang (a1), Y. D. Ni (a1), Y. S. Zhang (a1), S. Zhuang (a1) and X. Z. Shen (a1)...

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