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Genetic and morphology analysis among the pentaploid F1 hybrid fishes (Schizothorax wangchiachii ♀ × Percocypris pingi ♂) and their parents

  • H. R. Gu (a1), Y. F. Wan (a1), Y. Yang (a1), Q. Ao (a1), W. L. Cheng (a1), S. H. Deng (a2), D. Y. Pu (a1), X. F. He (a1), L. Jin (a1) and Z. J. Wang (a1)...


Triploid and pentaploid breeding is of great importance in agricultural production, but it is not always easy to obtain double ploidy parents. However, in fishes, chromosome ploidy is diversiform, which may provide natural parental resources for triploid and pentaploid breeding. Both tetraploid and hexaploid exist in Schizothorax fishes, which were thought to belong to different subfamilies with tetraploid Percocypris fishes in morphology, but they are sister genera in molecule. Fortunately, the pentaploid hybrid fishes have been successfully obtained by hybridization of Schizothorax wangchiachii (♀, 2n = 6X = 148) × Percocypris pingi (♂, 2n = 4X = 98). To understand the genetic and morphological difference among the hybrid fishes and their parents, four methods were used in this study: morphology, karyotype, red blood cell (RBC) DNA content determination and inter-simple sequence repeat (ISSR). In morphology, the hybrid fishes were steady, and between their parents with no obvious preference. The chromosome numbers of P. pingi have been reported as 2n = 4X = 98. In this study, the karyotype of S. wangchiachii was 2n = 6X = 148 = 36m + 34sm + 12st + 66t, while that the hybrid fishes was 2n = 5X = 123 = 39m + 28sm + 5st + 51t. Similarly, the RBC DNA content of the hybrid fishes was intermediate among their parents. In ISSR, the within-group genetic diversity of hybrid fishes was higher than that of their parents. Moreover, the genetic distance of hybrid fishes between P. pingi and S.wangchiachii was closely related to that of their parental ploidy, suggesting that parental genetic material stably coexisted in the hybrid fishes. This is the first report to show a stable pentaploid F1 hybrids produced by hybridization of a hexaploid and a tetraploid in aquaculture.


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Apostol, BL, Black, WC, Reiter, P and Miller, BR 1996. Population genetics with RAPD-PCR markers: the breeding structure of Aedes aegypti in Puerto Rico. Heredity 76, 325334.
Chen, C, Zhiguo, E and Lin, HX 2016. Evolution and molecular control of hybrid incompatibility in plants. Frontiers in Plant Science 7, 1208.
Chen, Y, Cheng, QQ, Qiao, HY, Zhu, YX, Chen, WM and Ren, GJ 2013. The complete mitochondrial genome sequence of Schizothorax wangchiachii (Cypriniformes: Cyprinidae). Mitochondrial DNA 24, 353355.
Ding, RH 1994. Fish fauna of Sichuan. Sichuan Science and Technology Press, Sichuan, China.
Foresti, F, Oliveira, C and Almeidatoledo, LFD 1993. A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine. Experientia 49, 810813.
He, WG, Qin, QB, Liu, SJ, Li, TL, Wang, J, Xiao, J, Xie, LH, Zhang, C and Liu, Y 2012. Organization and variation analysis of 5 S rDNA in different ploidy-level hybrids of red Crucian Carp × Topmouth Culter. Plos One 7, e38976.
Hu, J, Liu, SJ, Xiao, J, Zhou, Y, You, CP, He, WG, Zhao, RR, Song, C and Liu, Y 2012. Characteristics of diploid and triploid hybrids derived from female Megalobrama amblycephala Yih × male Xenocypris davidi Bleeker. Aquaculture 364, 157164.
Levan, A, Fredga, K and Sandberg, AA 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52, 201220.
Li, S, Tie, HM, Duan, J, Zhao, ZM, Yang, S, Guo, XL and Yang, SY 2017. The karyotype and C-banding of Percocypris pingi. Progress in Fishery Sciences 38, 1924.
Liu, SJ 2010. Distant hybridization leads to different ploidy fishes. Science China Life Sciences 53, 416425.
Liu, SJ, Liu, Y, Zhou, GJ, Zhang, XJ, Luo, C, Feng, H, He, XX, Zhou, GH and Yang, H 2001. The formation of tetraploid stocks of red crucian carp × common carp hybrids as an effect of interspecific hybridization. Aquaculture 192, 171186.
Liu, SJ, Qin, QB, Xiao, J, Lu, WT, Shen, JM, Li, W, Liu, JF, Duan, W, Zhang, C, Tao, M, Zhao, RR, Yan, JP and Liu, Y 2007. The formation of the polyploid hybrids from different subfamily fish crossings and its evolutionary significance. Genetics 176, 10231034.
Ma, X and Gustafson, JP 2005. Genome evolution of allopolyploids: a process of cytological and genetic diploidization. Cytogenetic Genome Research 109, 236249.
Meyer, A and Van de Peer, Y 2005. From 2R to 3R: evidence for a fish-specific genome duplication (FSGD). Bioessays 27, 937945.
Mugal, CF, Nabholz, B and Ellegren, H 2013. Genome-wide analysis in chicken reveals that local levels of genetic diversity are mainly governed by the rate of recombination. BMC Genomics 14, 86.
Nei, M and Li, WH 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences 76, 52695273.
Ozkan, H, Levy, AA and Feidman, M 2001. Allopolyploidy-inducedrapid genome evolution in the wheat (Aegilops-Triticum) group. Plant Cell 13, 17351747.
Piferrer, F, Beaumont, A, Falguiere, JC, Flajshans, M, Haffray, P and Colombo, L 2009. Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture 293, 125156.
Qin, QB, Wang, YD, Wang, J, Dai, J, Xiao, J, Hu, FZ, Luo, KY, Tao, M, Zhang, C, Liu, Y and Liu, SJ 2014. The autotetraploid fish derived from hybridization of Carassius auratus red var (Female) × Megalobrama amblycephala (Male). Biology of Reproduction 91, 111.
Song, C, Liu, SJ, Xiao, J, He, WG, Zhou, Y, Qin, QB, Zhang, C and Liu, Y 2012. Polyploid organisms. Science China Life Sciences 55, 301311.
Tao, Y, Zhang, ML, Ma, PC, Sun, JF, Zhou, WQ, Cao, YP and Li, LJ 2012. Triptolide inhibits proliferation and induces apoptosis of human melanoma A375 cells. Asian Pacific Journal of Cancer Prevention 13, 16111615.
Valérie, M 2008. The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish and Fisheries 9, 6778.
Venkatesh, B 2003. Evolution and diversity of fish genomes. Current Opinion in Genetics and Development 13, 588592.
Vogel, G 1998. Doubled genes may explain fish diversity. Science 281, 11191121.
Wang, M, Yang, JX and Chen, XY 2013. Molecular phylogeny and biogeography of Percocypris (Cyprinidae, Teleostei). Plos One 8, e61827.
Wang, S, Ye, XL, Wang, YD, Chen, YT, Lin, BW, Yi, ZF, Mao, ZW, Hu, FZ, Zhao, RR, Wang, J, Zhou, R, Ren, L, Yao, ZZ, Tao, M, Zhang, C, Xiao, J, Qin, QB and Liu, SJ 2017. A new type of homodiploid fish derived from the interspecific hybridization of female common carp × male blunt snout bream. Scientific Reports 7, 4189.
Wang, XZ, Gan, XN, Li, JP, Chen, YY and He, SP 2016. Cyprininae phylogeny revealed independent origins of the Tibetan plateau endemic polyploid cyprinids and their diversifications related to the neogene uplift of the plateau. Science China Life Sciences 59, 117.
Wang, YD, Yang, CH, Luo, KK, Zhang, MH, Qin, QB, Huo, YY, Song, J, Tao, M, Zhang, C and Liu, SJ 2018. The formation of the goldfish-like fish derived from hybridization of female Koi carp × male blunt snout bream. Frontiers in Genetics 9, 437.
Xiao, J, Kang, XW, Xie, LH, Qin, QB, He, ZL, Hu, FZ, Zhang, C, Zhao, RR, Wang, J, Luo, KK, Liu, Y and Liu, SJ 2014. The fertility of the hybrid lineage derived from female Megalobrama amblycephala × male Culter alburnus. Animal Reproduction Science 151, 6170.
Yang, L, Sado, T, Hirt, MV, Pasco-Viel, E, Arunachalam, M, Li, JB, Wang, XZ, Freyhof, J, Saitoh, K, Simons, AM, Miya, M, He, SP and Mayden, RL 2015. Phylogeny and polyploidy: resolving the classification of cyprinine fishes (Teleostei: Cypriniformes). Molecular Phylogenetics and Evolution 85, 97116.
Yang, Y, Chen, Y, Wan, YF, Deng, SH, He, XF, Wang, ZJ and Jin, L 2018. Embryonic, larval and juvenile development of hybrid between Schizothorax wangchiachii and Percocypris pingipingi. South China Fisheries Science 14, 6673.
Ye, LH, Jiao, N, Tang, XJ, Chen, YY, Ye, XL, Ren, L, Hu, FZ, Wang, S, Wen, M, Zhang, C, Tao, M and Liu, SJ 2017. Chimeras linked to tandem repeats and transposable elements in tetraploid hybrid fish. Marine Biotechnology 19, 401409.
Yeh, FC, Yang, RC and Boyle, T 1999. POPGENE. Microsoft Window-Based Freeware for population genetics analysis. University of Alberta, Edmonton. Retrieved on 12 April 2019 from
Yue, PQ 2000. FAUNA SINICA, Osteichthtes Cypriniformes III. Science Press, Beijing, China.
Zan, RG, Song, Z and Liu, WG 1984. Studies of karyotypes of seven species of fishes in barbinae, with a discussion on identification of fish polyqloidy. Zoological Research 5, 8793, 106107.
Zhang, ZH, Chen, J, Li, L, Tao, M, Zhang, C, Qin, QB, Xiao, J and Liu, SJ 2014. Research advances in animal distant hybridization. Science China Life Sciences 57, 889902.
Zhao, Y, Bao, ZM, Bi, K, Huang, XT, Wang, Y, Hu, JJ and Yang, AG 2006. Karyotypes of hybrid scallop(hybridizing cross the female Patinopecten yessoensis with the male Chlamys farreri) and their parents. Acta Oceanologica Sinica 28, 100105.
Zhou, RJ, Cheng, HH and Tiersch, TR 2001. Differential genome duplication and fish diversity. Reviews in Fish Biology and Fisheries 11, 331337.
Zietkiewicz, E, Rafalski, A and Labuda, D 1994. Genome Fingerprinting by Simple Sequence Repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176183.
Zou, SP, Fang, YL and Zhou, RQ 2008. Measurement of characters. Inspection of germplasm for cultured fishes, Part 3. Ministry of Agriculture of the People’s Republic of China GB/T 18654, 3-2008.


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Genetic and morphology analysis among the pentaploid F1 hybrid fishes (Schizothorax wangchiachii ♀ × Percocypris pingi ♂) and their parents

  • H. R. Gu (a1), Y. F. Wan (a1), Y. Yang (a1), Q. Ao (a1), W. L. Cheng (a1), S. H. Deng (a2), D. Y. Pu (a1), X. F. He (a1), L. Jin (a1) and Z. J. Wang (a1)...


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