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Construction of transgenic swine with induced expression of Cre recombinase

Published online by Cambridge University Press:  07 January 2010

L. Chen ,
L. Li ,
D. Pang ,
Z. Li ,
T. Wang ,
M. Zhang ,
N. Song ,
S. Yan ,
L. X. Lai  and
H. Ouyang
L. Chen
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China The Basis of the Faculty of Medicine, Department of BeiHua University, Jilin 132013, PR China
L. Li
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
D. Pang
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
Z. Li
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
T. Wang
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
M. Zhang
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
N. Song
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
S. Yan
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
L. X. Lai
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
H. Ouyang*
Affiliation:
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
*
†E-mail: ouyang_hongsheng@hotmail.com
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Abstract

Miniature pigs have been recognized as valuable experimental animals in medical research. However, porcine models related to gene knockout of human diseases are not widely available. The objective of this study was to establish Mx1-Cre pigs using somatic cell nuclear transfer. In this study, we created transgenic pigs using somatic cell nuclear transfer (SCNT). Transfer of 210, 230, 250 and 215 zygotes to four surrogates produced 10 piglets. The Cre recombinase expression in transgenic pigs was studied using reverse transcriptase (RT)-PCR and immunohistochemistry. Mx1-Cre swine were shown to harbor the Cre gene in their genomic DNA using the PCR. In conclusion, Mx1-Cre transgenic piglets were successfully produced by SCNT. These transgenic swine, in conjunction with inducible systems for controlling Cre expression and function, are likely to have a profound impact on the study of human diseases.

Keywords

Cre recombinase, somatic cell nuclear transfer (SCNT), pig
Type
Full Paper
Information
animal , Volume 4 , Issue 5 , May 2010 , pp. 767 - 771
Copyright
Copyright © The Animal Consortium 2009

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Footnotes

a

L. Chen and L. Li contributed equally to this work

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