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Cloning and characterization of the calreticulin gene in Asian seabass (Lates calcarifer)

Published online by Cambridge University Press:  17 November 2011

Z. Y. Bai ,
Z. Y. Zhu ,
C. M. Wang ,
J. H. Xia ,
X. P. He  and
G. H. Yue
Z. Y. Bai
Affiliation:
Temasek Life Sciences Laboratory, Molecular Population Genetics Group, National University of Singapore, 1 Research Link 117604, Singapore Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
Z. Y. Zhu
Affiliation:
Temasek Life Sciences Laboratory, Molecular Population Genetics Group, National University of Singapore, 1 Research Link 117604, Singapore
C. M. Wang
Affiliation:
Temasek Life Sciences Laboratory, Molecular Population Genetics Group, National University of Singapore, 1 Research Link 117604, Singapore
J. H. Xia
Affiliation:
Temasek Life Sciences Laboratory, Molecular Population Genetics Group, National University of Singapore, 1 Research Link 117604, Singapore
X. P. He
Affiliation:
Temasek Life Sciences Laboratory, Molecular Population Genetics Group, National University of Singapore, 1 Research Link 117604, Singapore
G. H. Yue*
Affiliation:
Temasek Life Sciences Laboratory, Molecular Population Genetics Group, National University of Singapore, 1 Research Link 117604, Singapore
*
†E-mail: genhua@tll.org.sg
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Abstract

Calreticulin (CRT) is a Ca2+-binding molecular chaperone in the endoplasmic reticulum. We cloned and characterized the CRT gene in an important marine food fish species Asian seabass (Lates calcarifer). The full-length DNA of the CRT gene was 2194 bp, including a complete open reading frame encoding 420 amino acid residues, a 113 bp 5′-untranslated region and an 818 bp 3′-untranslated region. The CRT gene contained nine exons and eight introns covering a total of 2772 bp genomic DNA from the start to stop codon. Ten single nucleotide polymorphisms (SNPs) were detected in introns and an exon in six individuals collected from five different locations. The CRT gene was assigned to linkage group 4 of the linkage map of Asian seabass. Quantitative real-time PCR revealed that the CRT gene was highly expressed in liver at the age of 1, 3 and 7 months under normal conditions, whereas its expression in liver reduced sharply after 0.5 to 2 h cold challenge at 16°C, and then increased slowly. A preliminary association analysis showed a significant (P < 0.001) association between the SNP6 in the CRT gene and the mortality after cold challenge at 16°C. Our results suggest that the CRT gene is associated with cold tolerance of Asian seabass and further investigation will be necessary to illustrate the underlying mechanisms.

Keywords

Barramundipolymorphismcold toleranceQTL
Type
Full Paper
Information
animal , Volume 6 , Issue 6 , June 2012 , pp. 887 - 893
Copyright
Copyright © The Animal Consortium 2011

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Supplementary material: File

Bai Supplementary Figure

Fig. S1 Nucleotide and deduced amino acid sequence of seabass calreticulin(CRT) cDNA

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