Skip to main content Accessibility help
×
Home
Hostname: page-component-559fc8cf4f-7x8lp Total loading time: 0.953 Render date: 2021-02-25T08:39:05.302Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }

Article contents

Genetic and morphology analysis among the pentaploid F1 hybrid fishes (Schizothorax wangchiachii ♀ × Percocypris pingi ♂) and their parents

Published online by Cambridge University Press:  31 May 2019

H. R. Gu
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
Y. F. Wan
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
Y. Yang
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
Q. Ao
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
W. L. Cheng
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
S. H. Deng
Affiliation:
Xichang Jiahe Agriculture Company Limited, Xichang 615000, China
D. Y. Pu
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
X. F. He
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
L. Jin
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
Z. J. Wang
Affiliation:
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
Corresponding
E-mail address:
Get access

Abstract

Triploid and pentaploid breeding is of great importance in agricultural production, but it is not always easy to obtain double ploidy parents. However, in fishes, chromosome ploidy is diversiform, which may provide natural parental resources for triploid and pentaploid breeding. Both tetraploid and hexaploid exist in Schizothorax fishes, which were thought to belong to different subfamilies with tetraploid Percocypris fishes in morphology, but they are sister genera in molecule. Fortunately, the pentaploid hybrid fishes have been successfully obtained by hybridization of Schizothorax wangchiachii (♀, 2n = 6X = 148) × Percocypris pingi (♂, 2n = 4X = 98). To understand the genetic and morphological difference among the hybrid fishes and their parents, four methods were used in this study: morphology, karyotype, red blood cell (RBC) DNA content determination and inter-simple sequence repeat (ISSR). In morphology, the hybrid fishes were steady, and between their parents with no obvious preference. The chromosome numbers of P. pingi have been reported as 2n = 4X = 98. In this study, the karyotype of S. wangchiachii was 2n = 6X = 148 = 36m + 34sm + 12st + 66t, while that the hybrid fishes was 2n = 5X = 123 = 39m + 28sm + 5st + 51t. Similarly, the RBC DNA content of the hybrid fishes was intermediate among their parents. In ISSR, the within-group genetic diversity of hybrid fishes was higher than that of their parents. Moreover, the genetic distance of hybrid fishes between P. pingi and S.wangchiachii was closely related to that of their parental ploidy, suggesting that parental genetic material stably coexisted in the hybrid fishes. This is the first report to show a stable pentaploid F1 hybrids produced by hybridization of a hexaploid and a tetraploid in aquaculture.

Type
Research Article
Information
Copyright
© The Animal Consortium 2019 

Access options

Get access to the full version of this content by using one of the access options below.

References

Apostol, BL, Black, WC, Reiter, P and Miller, BR 1996. Population genetics with RAPD-PCR markers: the breeding structure of Aedes aegypti in Puerto Rico. Heredity 76, 325334.10.1038/hdy.1996.50CrossRefGoogle ScholarPubMed
Chen, C, Zhiguo, E and Lin, HX 2016. Evolution and molecular control of hybrid incompatibility in plants. Frontiers in Plant Science 7, 1208.CrossRefGoogle ScholarPubMed
Chen, Y, Cheng, QQ, Qiao, HY, Zhu, YX, Chen, WM and Ren, GJ 2013. The complete mitochondrial genome sequence of Schizothorax wangchiachii (Cypriniformes: Cyprinidae). Mitochondrial DNA 24, 353355.10.3109/19401736.2012.760556CrossRefGoogle Scholar
Ding, RH 1994. Fish fauna of Sichuan. Sichuan Science and Technology Press, Sichuan, China.Google Scholar
Foresti, F, Oliveira, C and Almeidatoledo, LFD 1993. A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine. Experientia 49, 810813.10.1007/BF01923555CrossRefGoogle Scholar
He, WG, Qin, QB, Liu, SJ, Li, TL, Wang, J, Xiao, J, Xie, LH, Zhang, C and Liu, Y 2012. Organization and variation analysis of 5 S rDNA in different ploidy-level hybrids of red Crucian Carp × Topmouth Culter. Plos One 7, e38976.CrossRefGoogle Scholar
Hu, J, Liu, SJ, Xiao, J, Zhou, Y, You, CP, He, WG, Zhao, RR, Song, C and Liu, Y 2012. Characteristics of diploid and triploid hybrids derived from female Megalobrama amblycephala Yih × male Xenocypris davidi Bleeker. Aquaculture 364, 157164.CrossRefGoogle Scholar
Levan, A, Fredga, K and Sandberg, AA 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52, 201220.CrossRefGoogle Scholar
Li, S, Tie, HM, Duan, J, Zhao, ZM, Yang, S, Guo, XL and Yang, SY 2017. The karyotype and C-banding of Percocypris pingi. Progress in Fishery Sciences 38, 1924.Google Scholar
Liu, SJ 2010. Distant hybridization leads to different ploidy fishes. Science China Life Sciences 53, 416425.CrossRefGoogle ScholarPubMed
Liu, SJ, Liu, Y, Zhou, GJ, Zhang, XJ, Luo, C, Feng, H, He, XX, Zhou, GH and Yang, H 2001. The formation of tetraploid stocks of red crucian carp × common carp hybrids as an effect of interspecific hybridization. Aquaculture 192, 171186.CrossRefGoogle Scholar
Liu, SJ, Qin, QB, Xiao, J, Lu, WT, Shen, JM, Li, W, Liu, JF, Duan, W, Zhang, C, Tao, M, Zhao, RR, Yan, JP and Liu, Y 2007. The formation of the polyploid hybrids from different subfamily fish crossings and its evolutionary significance. Genetics 176, 10231034.CrossRefGoogle ScholarPubMed
Ma, X and Gustafson, JP 2005. Genome evolution of allopolyploids: a process of cytological and genetic diploidization. Cytogenetic Genome Research 109, 236249.10.1159/000082406CrossRefGoogle ScholarPubMed
Meyer, A and Van de Peer, Y 2005. From 2R to 3R: evidence for a fish-specific genome duplication (FSGD). Bioessays 27, 937945.10.1002/bies.20293CrossRefGoogle Scholar
Mugal, CF, Nabholz, B and Ellegren, H 2013. Genome-wide analysis in chicken reveals that local levels of genetic diversity are mainly governed by the rate of recombination. BMC Genomics 14, 86.CrossRefGoogle Scholar
Nei, M and Li, WH 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences 76, 52695273.10.1073/pnas.76.10.5269CrossRefGoogle ScholarPubMed
Ozkan, H, Levy, AA and Feidman, M 2001. Allopolyploidy-inducedrapid genome evolution in the wheat (Aegilops-Triticum) group. Plant Cell 13, 17351747.Google ScholarPubMed
Piferrer, F, Beaumont, A, Falguiere, JC, Flajshans, M, Haffray, P and Colombo, L 2009. Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture 293, 125156.CrossRefGoogle Scholar
Qin, QB, Wang, YD, Wang, J, Dai, J, Xiao, J, Hu, FZ, Luo, KY, Tao, M, Zhang, C, Liu, Y and Liu, SJ 2014. The autotetraploid fish derived from hybridization of Carassius auratus red var (Female) × Megalobrama amblycephala (Male). Biology of Reproduction 91, 111.CrossRefGoogle Scholar
Song, C, Liu, SJ, Xiao, J, He, WG, Zhou, Y, Qin, QB, Zhang, C and Liu, Y 2012. Polyploid organisms. Science China Life Sciences 55, 301311.10.1007/s11427-012-4310-2CrossRefGoogle ScholarPubMed
Tao, Y, Zhang, ML, Ma, PC, Sun, JF, Zhou, WQ, Cao, YP and Li, LJ 2012. Triptolide inhibits proliferation and induces apoptosis of human melanoma A375 cells. Asian Pacific Journal of Cancer Prevention 13, 16111615.CrossRefGoogle ScholarPubMed
Valérie, M 2008. The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish and Fisheries 9, 6778.Google Scholar
Venkatesh, B 2003. Evolution and diversity of fish genomes. Current Opinion in Genetics and Development 13, 588592.CrossRefGoogle ScholarPubMed
Vogel, G 1998. Doubled genes may explain fish diversity. Science 281, 11191121.CrossRefGoogle ScholarPubMed
Wang, M, Yang, JX and Chen, XY 2013. Molecular phylogeny and biogeography of Percocypris (Cyprinidae, Teleostei). Plos One 8, e61827.CrossRefGoogle Scholar
Wang, S, Ye, XL, Wang, YD, Chen, YT, Lin, BW, Yi, ZF, Mao, ZW, Hu, FZ, Zhao, RR, Wang, J, Zhou, R, Ren, L, Yao, ZZ, Tao, M, Zhang, C, Xiao, J, Qin, QB and Liu, SJ 2017. A new type of homodiploid fish derived from the interspecific hybridization of female common carp × male blunt snout bream. Scientific Reports 7, 4189.CrossRefGoogle ScholarPubMed
Wang, XZ, Gan, XN, Li, JP, Chen, YY and He, SP 2016. Cyprininae phylogeny revealed independent origins of the Tibetan plateau endemic polyploid cyprinids and their diversifications related to the neogene uplift of the plateau. Science China Life Sciences 59, 117.10.1007/s11427-016-0007-7CrossRefGoogle ScholarPubMed
Wang, YD, Yang, CH, Luo, KK, Zhang, MH, Qin, QB, Huo, YY, Song, J, Tao, M, Zhang, C and Liu, SJ 2018. The formation of the goldfish-like fish derived from hybridization of female Koi carp × male blunt snout bream. Frontiers in Genetics 9, 437.CrossRefGoogle ScholarPubMed
Xiao, J, Kang, XW, Xie, LH, Qin, QB, He, ZL, Hu, FZ, Zhang, C, Zhao, RR, Wang, J, Luo, KK, Liu, Y and Liu, SJ 2014. The fertility of the hybrid lineage derived from female Megalobrama amblycephala × male Culter alburnus. Animal Reproduction Science 151, 6170.10.1016/j.anireprosci.2014.09.012CrossRefGoogle ScholarPubMed
Yang, L, Sado, T, Hirt, MV, Pasco-Viel, E, Arunachalam, M, Li, JB, Wang, XZ, Freyhof, J, Saitoh, K, Simons, AM, Miya, M, He, SP and Mayden, RL 2015. Phylogeny and polyploidy: resolving the classification of cyprinine fishes (Teleostei: Cypriniformes). Molecular Phylogenetics and Evolution 85, 97116.CrossRefGoogle Scholar
Yang, Y, Chen, Y, Wan, YF, Deng, SH, He, XF, Wang, ZJ and Jin, L 2018. Embryonic, larval and juvenile development of hybrid between Schizothorax wangchiachii and Percocypris pingipingi. South China Fisheries Science 14, 6673.Google Scholar
Ye, LH, Jiao, N, Tang, XJ, Chen, YY, Ye, XL, Ren, L, Hu, FZ, Wang, S, Wen, M, Zhang, C, Tao, M and Liu, SJ 2017. Chimeras linked to tandem repeats and transposable elements in tetraploid hybrid fish. Marine Biotechnology 19, 401409.CrossRefGoogle ScholarPubMed
Yeh, FC, Yang, RC and Boyle, T 1999. POPGENE. Microsoft Window-Based Freeware for population genetics analysis. University of Alberta, Edmonton. Retrieved on 12 April 2019 from http://www.ualberta.ca/~fyeh/index.htmGoogle Scholar
Yue, PQ 2000. FAUNA SINICA, Osteichthtes Cypriniformes III. Science Press, Beijing, China.Google Scholar
Zan, RG, Song, Z and Liu, WG 1984. Studies of karyotypes of seven species of fishes in barbinae, with a discussion on identification of fish polyqloidy. Zoological Research 5, 8793, 106107.Google Scholar
Zhang, ZH, Chen, J, Li, L, Tao, M, Zhang, C, Qin, QB, Xiao, J and Liu, SJ 2014. Research advances in animal distant hybridization. Science China Life Sciences 57, 889902.10.1007/s11427-014-4707-1CrossRefGoogle ScholarPubMed
Zhao, Y, Bao, ZM, Bi, K, Huang, XT, Wang, Y, Hu, JJ and Yang, AG 2006. Karyotypes of hybrid scallop(hybridizing cross the female Patinopecten yessoensis with the male Chlamys farreri) and their parents. Acta Oceanologica Sinica 28, 100105.Google Scholar
Zhou, RJ, Cheng, HH and Tiersch, TR 2001. Differential genome duplication and fish diversity. Reviews in Fish Biology and Fisheries 11, 331337.CrossRefGoogle Scholar
Zietkiewicz, E, Rafalski, A and Labuda, D 1994. Genome Fingerprinting by Simple Sequence Repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176183.CrossRefGoogle ScholarPubMed
Zou, SP, Fang, YL and Zhou, RQ 2008. Measurement of characters. Inspection of germplasm for cultured fishes, Part 3. Ministry of Agriculture of the People’s Republic of China GB/T 18654, 3-2008.Google Scholar

Gu et al. supplementary material

Gu et al. supplementary material 1

File 559 KB

Altmetric attention score

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 17
Total number of PDF views: 101 *
View data table for this chart

* Views captured on Cambridge Core between 31st May 2019 - 25th February 2021. This data will be updated every 24 hours.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Genetic and morphology analysis among the pentaploid F1 hybrid fishes (Schizothorax wangchiachii ♀ × Percocypris pingi ♂) and their parents
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Genetic and morphology analysis among the pentaploid F1 hybrid fishes (Schizothorax wangchiachii ♀ × Percocypris pingi ♂) and their parents
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Genetic and morphology analysis among the pentaploid F1 hybrid fishes (Schizothorax wangchiachii ♀ × Percocypris pingi ♂) and their parents
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *