Skip to main content Accessibility help

Plasma non-esterified fatty acid response to a β-adrenergic challenge before or after feeding in energy underfed or overfed, dry or lactating cows

  • Y. Chilliard (a1), A. Ferlay (a1), L. Desprès (a1) and F. Bocquier (a1)


The effects of adrenergic challenge (in order to evaluate the adipose tissue lipolytic potential) and time of challenge relative to feeding on the response curve of plasma non-esterified fatty acids (NEFA) in four underfed or overfed non-lactating non-pregnant cows were studied. Basal NEFA and NEFA response to isoproterenol (ISO; 4 nmol/kg body weight) were higher when challenged before than after feeding and higher in underfed than overfed cows. Interaction between feeding level and time of challenge was significant (P < 0·001) for the rising part of NEFA response. Pooled NEFA response curves from several trials (no. = 63 challenges) were analysed in order to obtain a simplified procedure for the prediction of the NEFA response. High correlations were found between the response area or maximal NEFA response and NEFA response at 15 min (r = 0·95 and 0·98, respectively). This simplified procedure was applied on pooled data from 84 challenges in order to evaluate the effects of energy balance, physiological status, body condition score (BCS) and time of challenge relative to feeding. Energy balance had a significant effect on basal plasma NEFA and NEFA response at 15 min after ISO challenge (–7·6 and –14·2 μmol/l when the daily energy balance increased by 1 MJ, respectively). Time of ISO injection relative to feeding had a greater effect on stimulated NEFA than on basal NEFA (308 v. 239 μmol/l). There was a significant effect of BCS (41 μmol/l per unit of BCS) on the basal plasma NEFA level. The NEFA response at 15 min after ISO challenge was lower (387 μmol/l) in lactating cows than in dry cows. The NEFA response to ISO at 15 min could provide an efficient method of studying the adipose tissue lipolytic potential of cattle in vivo.



Hide All
Bareille, N., Faverdin, P. and Hay, M. 1997. Modification of feed intake response to a β2-agonist by bovine somatotropin in lactating or dry dairy cows. Journal of Dairy Science 80: 5266.
Bassett, J. M. 1974. Diurnal patterns of plasma insulin, growth hormone, corticosteroid and metabolite concentrations in fed and fasted sheep. Australian Journal of Biological Sciences 27: 167181.
Bauman, D. E. and Elliot, J. M. 1983. Control of nutrient partitioning in lactating ruminants. In Biochemistry of lactation (ed. Mepham, T. B.), pp. 437468. Elsevier Science Publishers, Amsterdam.
Bines, J. A. and Morant, S. V. 1983. The effect of body condition on metabolic changes associated with intake of food by the cow. British Journal of Nutrition1 50: 8189.
Blum, J. W., Fröhli, D. and Kunz, P. 1989 Effects of catecholamines on plasma free fatty acids in fed and fasted cattle. Endocrinology 110: 452456.
Bocquier, F., Ferlay, A. and Chilliard, Y. 1998. Effects of body lipids and energy balance on the response of plasma non-esterified fatty acids to a β-adrenergic challenge in the lactating dairy ewe. In Energy metabolism of farm animals (ed. McCracken, K. K., Unsworth, E. F. and Wylie, A. R. G.), proceedings of the 14th symposium on energy metabolism in farm animals, Newcastle, Northern Ireland, Sept. 14-20, 1997, pp. 167170. CAB International, University Press, Cambridge.
Bouyekhf, M., Rule, D. C. and Yuan Hu, C. 1993. Effect of catecholamines on lipolysis and esterification in vitro in adipose tissue of sheep fed low and high energy diets. Journal of Nutritional Biochemistry 4: 8085.
Chilliard, Y. 1992. Physiological constraints to milk production: factors which determine nutrient partitioning, lactation persistency, and mobilization of body reserves. World Review of Animal Production 23: 2025.
Chilliard, Y., Bauchart, D. and Barnouin, J. 1984a. Determination of plasma non esterified fatty acids in herbivores and man: a comparison of values obtained by manual or automatic chromatographic, titrimetric, colorimetric and enzymatic methods. Reproduction, Nutrition, Développement 24: 469482.
Chilliard, Y. and Bocquier, F. 1992. Glucose, NEFA and glycerol responses to beta- or alpha-2-adrenergic challenges in the underfed dry non-pregnant cow receiving, or not, bST. Journal of Dairy Science 75: (suppl. 1) 236.
Chilliard, Y., Bocquier, F. and Sala, A. M. 1992. Ruminant undernutrition. Adipose tissue metabolism in vivo: straw-based diets; effects of growth hormone. INRA, Center of Clermont-Fd-Theix, research report 1990-1992, pp. 128129.
Chilliard, Y., Doreau, M., Bocquier, F. and Lobley, G. E. 1995. Digestive and metabolic adaptations of ruminants to variations in food supply. In Recent developments in the nutrition of herbivores (ed. Journet, M., Grenet, E., Farce, M. H., Thériez, M. and Demarquilly, C.), proceedings of the IVth international symposium on the nutrition of herbivores, pp. 329360. INRA Editions, Paris.
Chilliard, Y. and Ottou, J. F. 1995. Duodenal infusion of oil in midlactation cows. 7. Interaction with niacin on responses to glucose, insulin and β-agonist challenges. Journal of Dairy Science 78: 24522463.
Chilliard, Y., Robelin, J. and Remond, B. 1984b. In vivo estimation of body lipid mobilization and reconstitution in dairy cattle. Canadian Journal of Animal Science 64: (supplement) 236237.
Chilliard, Y., Sauvant, D., Morand-Fehr, P. and Delouis, C. 1987. Relations entre le bilan énergétique et l'activité métabolique du tissu adipeux de la chèvre au cours de la première moitié de la lactation. Reproduction, Nutrition, Développement 27: 307308.
Cissé, M., Chilliard, Y., Coxam, V., Davicco, M. J. and Rémond, B. 1991. Slow release somatotropin in dairy heifers and cows fed two levels of energy concentrate. 2. Plasma hormones and metabolites. Journal of Dairy Science 74: 13821394.
Coppack, S. W., Evans, R. D., Fisher, R. M., Frayn, K. N., Gibbons, G. F., Humphreys, S. M., Kirk, M. L., Potts, J. L. and Hockaday, T. D. R. 1992. Adipose tissue metabolism in obesity: lipase action in vivo before and after a mixed meal. Metabolism 41: 264272.
DiMarco, N. M., Rule, D. C., Whitehurst, G. B. and Beitz, D. C. 1991. Effect of indomethacin, epinephrine, prostaglandin E 2 and insulin on lipolysis in bovine adipose tissue in vitro. International Journal of Biochemistry 23: 12311235.
DiMarco, N. M., Whitehurst, G. B. and Beitz, D. C. 1986. Evaluation of prostaglandin E2 as a regulator of lipolysis in bovine adipose tissue. Journal of Animal Science 62: 363369.
Doreau, M. 1983. Influence de la prise alimentaire sur les variations de différents constituants plasmatiques chez la vache en fin de gestation et en debut de lactation. Annales de Recherches Vétérinaires 14: 3948.
Dunshea, F. R., Bell, A. W. and Trigg, T. E. 1988. Relations between plasma non-esterified fatty acid metabolism and body tissue mobilization during chronic undernutrition in goats. British Journal of Nutrition 60: 633644.
Dunshea, F. R., Bell, A. W. and Trigg, T. E. 1990. Non-esterified fatty acid and glycerol kinetics and fatty acid re-esterification in goats during early lactation. British Journal of Nutrition 64: 133145.
Ferlay, A., Chilliard, Y., Sala, A. M., Durier, C. and Bocquier, F. 1996. Somatotropin treatment does not affect non-esterified fatty acid response to adrenergic injections in underfed or overfed non-lactating cows. Journal of Nutrition 126: 945954.
Fröhli, D. M. and Blum, J. W. 1988. Non-esterified fatty acids and glucose in lactating dairy cows: diurnal variations and changes in responsiveness during fasting to epinephrine and effects of beta-adrenergic blockade. Journal of Dairy Science 71: 11701177.
Gagliostro, G. and Chilliard, Y. 1991. Duodenal rapeseed oil infusion in early and midlactation cows. 4. In vivo and in vitro adipose tissue lipolytic responses. Journal of Dairy Science 74: 18301843.
Garnsworthy, P. C. 1988. The effect of energy reserves at calving on performance of dairy cows. In Nutrition and lactation in the dairy cow (ed. Garnsworthy, P. C.), proceedings of the 46th University of Nottingham easter school in agricultural science, pp. 157170.
Guesnet, P., Massoud, M. and Demarne, Y. 1987. Effects of pregnancy and lactation on lipolysis of ewe adipocytes induced by β-adrenergic stimulation. Molecular and Cellular Endocrinology 50: 177181.
Holmes, J. H. G. and Lambourne, L. J. 1970. The relation between plasma free fatty acid concentration and the digestible energy intake of cattle. Research in Veterinary Science 11: 2736.
Houseknecht, K. L., Bauman, D. E., Carey, G. B. and Mersmann, H. J. 1995. Effect of bovine somatotropin and food deprivation on β-adrenergic and Al adenosine receptor binding in adipose tissue of lactating cows. Domestic Animal Endocrinology 12: 325336.
Institut National de la Recherche Agronomique. 1989. Ruminant nutrition. Recommended allowances and feed tables (ed. Jarrige, R.). John Libbey Eurotext, London.
Jaster, E. H. and Wegner, R. N. 1981. Beta-adrenergic receptor involvement in lipolysis of dairy cattle subcutaneous adipose tissue during nonlactating and lactating states. Journal of Dairy Science 64: 16551663.
Kronfeld, D. S. 1965. Plasma non-esterified fatty acid concentrations in the dairy cow: responses to nutritional and hormonal stimuli and significance in ketosis. Veterinary Record 77: 3034.
Lafontan, M. 1994. Mini review. Differential recruitment and differential regulation by physiological amines of fat cell β-1, β-2 and β-3 adrenergic receptors expressed in native fat cells and in transfected cell lines. Cellular Signalling 6: 363392.
Langin, D., Holm, C. and Lafontan, M. 1996. Adipocyte hormone-sensitive lipase: a major regulator of lipid metabolism. Proceedings of the Nutrition Society 55: 93109.
McCutcheon, S. N. and Bauman, D. E. 1986. Effect of chronic growth hormone treatment on responses to epinephrine and thyrotropin-releasing hormone in lactating cows. Journal of Dairy Science 69: 4451.
McNamara, J. P. 1994. Lipid metabolism in adipose tissue during lactation: a model of a metabolic control system. Journal of Nutrition 124: 1383S1391S.
Peters, J. P. 1986. Consequences of accelerated gain and growth hormone administration for lipid metabolism in growing beef steers. Journal of Nutrition 116: 24902503.
Pethick, D. W. and Dunshea, F. R. 1993. Fat metabolism and turnover. In CAB International quantitative aspects of ruminant digestion and metabolism (ed. Forbes, J. M. and France, J.), pp. 292311. CAB International, Wallingford, Oxfordshire.
Petterson, J. A., Slepetid, R., Ehrhardt, R. A., Dunshea, F. R. and Bell, A. W. 1994. Pregnancy but not moderate undernutrition attenuates insulin suppression of fat mobilization in sheep. Journal of Nutrition 124: 24312436.
Pothoven, M. A., Beitz, D. C. and Thornton, J. H. 1975. Lipogenesis and lipolysis in adipose tissue of ad libitum and restricted-fed beef cattle during growth. Journal of Animal Science 40: 957962.
Sechen, S. J., Dunshea, F. R. and Bauman, D. E. 1990. Somatotropin in lactating cows: effect on response to epinephrine and insulin. American Journal of Physiology 258: E582–E588.
Sechen, S. J., McCutcheon, S. N. and Bauman, D. E. 1989. Response to metabolic challenges in early lactation dairy cows during treatment with bovine somatotropin. Domestic Animal Endocrinology 6: 141154.
Statistical Analysis Systems Institute. 1987. User's guide: statistics, version 6 edition. Statistical Analysis Systems Institute Inc., Cary, NC.
Vernon, R. G. 1992. Effects of diet on lipolysis and its regulation. Proceedings of the Nutrition Society 51: 397408.
Vernon, R. G., Finley, E. and Watt, P. W. 1991. Adenosine and the control of adrenergic regulation of adipose tissue lipolysis during lactation. Journal of Dairy Science 74: 695705.
Xing, G. Q., Mackenzie, D. D. S. and McCutcheon, S. N. 1991. Diurnal variation in plasma metabolite and hormone concentrations and response to metabolic challenges in high breeding index and low breeding index Friesian heifers fed at two allowances. New Zealand Journal of Agricultural Research 34: 295304.


Related content

Powered by UNSILO

Plasma non-esterified fatty acid response to a β-adrenergic challenge before or after feeding in energy underfed or overfed, dry or lactating cows

  • Y. Chilliard (a1), A. Ferlay (a1), L. Desprès (a1) and F. Bocquier (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.