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Follicular growth and serum follicle-stimulating hormone (FSH) responses to recombinant bovine FSH in GnRH-immunized anoestrous heifers

Published online by Cambridge University Press:  18 August 2016

M. A. Crowe ,
W. J. Enright ,
M. P. Boland  and
J. F. Roche
M. A. Crowe*
Affiliation:
Faculties of Veterinary Medicine
W. J. Enright*
Affiliation:
Faculties of Teagasc, Grange Research Centre, Dunsany, Co. Meath, Ireland
M. P. Boland
Affiliation:
Faculties of Agriculture, University College Dublin, Ireland
J. F. Roche
Affiliation:
Faculties of Veterinary Medicine
*
†Correspondence to Department of Animal Husbandry and Production, Faculty of Veterinary Medicine, University College Dublin, Ballsbridge, Dublin 4, Ireland. E-mail: MCrowe@ucd.ie
‡Present address: Intervet International B. V., Wim de Korverstraat 35, PO Box 31, 5830 AA Boxmeer, The Netherlands.
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Abstract

The effect of recombinant bovine FSH (rbFSH) administration on serum FSH and follicular growth was determined in 16 GnRH-immunized heifers (primary day 0 and booster day 28 using 0·4 mg human serum albumin (HSA) – Cys-Gly-GnRH in DEAE-dextran). All heifers produced GnRH antibody titres (mean 58·8 (s.e. 2·8)% binding at 1: 640 serum dilution on day 42), follicular growth ceased (follicles ≤ 4 mm) between days 33 and 49, progesterone concentrations were ≤ 0·5 ng/ml in all heifers by day 53, and pulsatile LH secretion was markedly reduced (v. luteal phase control heifers) on day 63. Heifers were blocked by antibody titre and LH concentrations on day 63 and assigned (no. = five or six per treatment) on day 78 to: (1) control (saline given i.m. four times per day for 4 days), (2) 0·5 mg equivalent (USDA bFSH BP 1) of rbFSH (Granada Biosciences, TX) given i.m. four times per day for 6 days (12 mg in total) or 3) 1·5 mg rbFSH given i.m. four times per day for 4 days (24 mg in total). Ovaries of heifers were examined by ultrasound and blood samples were collected at 3- to 6-h intervals from days –1 to 10 and then twice a day until follicles ≥ 5 mm were no longer detectable. The increase in serum FSH concentrations was different (P < 0·05) between treatments (mean peak FSH above pre-treatment baseline concentrations at 102 h after first rbFSH were 1·4 (s.e. 0·8), 14·5 (s.e. 3·3) and 33·6 (s.e. 5·3) ng/ml for treatments 1 to 3, respectively). The rate of decline in FSH from 102 to 302 h was not different between treatments 2 and 3. There was no detectable follicular growth in heifers given saline injections. Following either rbFSH treatment, medium (MF; 5 to 9 mm) and large (LF; ≥ 10 mm) follicles grew. For heifers in treatments 2 and 3, there was no difference in the interval from start of rbFSH until the first appearance of MF (4·2 (s.e. 0·7) v. 3·3 (s.e. 0·3) days) or LF (5·3 (s.e. 0·3) v. 4·8 (s.e. 0·8) days) or in the duration of the presence of MF and/or LF (13·2 (s.e. 4·5) v. 16·6 (s.e. 2·6) days). Increasing dose of rbFSH increased (P < 0·05) the mean maximum number of MF (4·3 (s.e. 1·1) and 9·2 (s.e.0·9) for treatments 2 and 3) and LF (2·0 (s.e. 1·1) and 8·4 (s.e. 1·2)). In summary, low doses of rbFSH induced a small cohort of follicles to grow and develop without selection or development of a single dominant follicle, whereas high doses of rbFSH resulted in large numbers of both MF and LF.

Keywords

folliclesFSHGnRHheifersimmunization.
Type
Reproduction
Information
Animal Science , Volume 73 , Issue 1 , August 2001 , pp. 115 - 122
Copyright
Copyright © British Society of Animal Science 2001

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References

Adams, G. P., Matteri, R. L., Kastelic, J. P., Ko, J. C. H and Ginther, O. J. 1992. Association between surges of follicle-stimulating hormone and the emergence of follicular waves in heifers. Journal of Reproduction and Fertility 94: 177–188.CrossRefGoogle ScholarPubMed
Adams, T. E. and Adams, B. M. 1986. Gonadotrope function in ovariectomized ewes actively immunized against gonadotropin-releasing hormone (GnRH). Biology of Reproduction 35: 360–367.CrossRefGoogle ScholarPubMed
Bishop, D. K., Wettemann, R. P., Yelich, J. V. and Spicer, L J. 1996. Ovarian response after gonadotropin treatment of heifers immunized against gonadotropin-releasing hormone. Journal of Animal Science 74: 1092–1097.CrossRefGoogle ScholarPubMed
Campbell, B. K., Scaramuzzi, R. J. and Webb, R. 1995. Control of antral follicle development and selection in sheep and cattle. Journal of Reproduction and Fertility, Supplement 49: 335–350.Google ScholarPubMed
Crowe, M. A., Enright, W. J., Prendiville, D. J., Morrison, C. A. and Roche, J. F. 1994. Active immunization against prostaglandin F2a: effect of conjugate dose and booster interval on antibody titers and estrous behavior in postpubertal beef heifers. Journal of Animal Science 72: 1778–1785.CrossRefGoogle ScholarPubMed
Crowe, M. A., Padmanabhan, V., Hynes, N., Sunderland, S. J., Enright, W. J., Beitins, I. Z. and Roche, J. F. 1997. Validation of a sensitive radioimmunoassay to measure serum follicle-stimulating hormone in cattle: correlation with biological activity. Animal Reproduction Science 48: 123–136.CrossRefGoogle ScholarPubMed
Finnerty, M., Enright, W. J., Morrison, C. A. and Roche, J. F. 1994. Immunization of bull calves with a GnRH analogue-human serum albumin conjugate: effect of conjugate dose, type of adjuvant and booster interval on immune, endocrine, testicular and growth responses. Journal of Reproduction and Fertility 101: 333–343.CrossRefGoogle ScholarPubMed
Gong, J. G., Bramley, T. A., Gutierrez, C. G., Peters, A. R. and Webb, R. 1995. Effects of chronic treatment with a gonadotrophin-releasing hormone agonist on peripheral concentrations of FSH and LH, and ovarian function in heifers. Journal of Reproduction and Fertility 105: 263–270.CrossRefGoogle ScholarPubMed
Gong, J. G., Campbell, B. K., Bramley, T. A., Gutierrez, C. G., Peters, A. R. and Webb, R. 1996. Suppression in the secretion of follicle-stimulating hormone and luteinizing hormone, and ovarian follicle development in heifers continuously infused with a gonadotrophin-releasing hormone agonist. Biology of Reproduction 55: 68–74.CrossRefGoogle Scholar
Knopf, L., Kastelic, J. P., Schallenberger, E. and Ginther, O. J. 1989. Ovarian follicular dynamics in heifers: test of two-wave hypothesis by ultrasonically monitoring individual follicles. Domestic Animal Endocrinology 6: 111–119.CrossRefGoogle ScholarPubMed
Murphy, M. G., Boland, M. P. and Roche, J. F. 1990. Pattern of follicular growth and resumption of ovarian activity in post-partum beef suckler cows. Journal of Reproduction and Fertility 90: 523–533.CrossRefGoogle ScholarPubMed
Niswender, G. D., Reichert, L. E. Jr.,, Midgley Jr, A. R. and Nalbandov, A. V. 1969. Radioimmunoassay for bovine and ovine luteinizing hormone. Endocrinology 84: 1166–1173.CrossRefGoogle ScholarPubMed
Prendiville, D. J., Enright, W. J., Crowe, M. A., Finnerty, M., Hynes, N. and Roche, J. F. 1995a. Immunization of heifers against gonadotropin-releasing hormone: antibody titers, ovarian function, body growth, and carcass characteristics. Journal of Animal Science 73: 2382–2389.CrossRefGoogle ScholarPubMed
Prendiville, D. J., Enright, W. J., Crowe, M. A., Vaughan, L. and Roche, J. F. 1995b. Immunization of prepubertal beef heifers against gonadotropin-releasing hormone: immune, estrus, ovarian, and growth responses. Journal of Animal Science 73: 3030–3037.CrossRefGoogle ScholarPubMed
Prendiville, D. J., Enright, W. J., Crowe, M. A., Finnerty, M. and Roche, J. F. 1996. Normal or induced secretory patterns of luteinising hormone and follicle-stimulating hormone in anoestrous gonadotrophin-releasing hormone-immunized and cyclic control heifers. Animal Reproduction Science 45: 177–190.CrossRefGoogle ScholarPubMed
Ronayne, E. and Hynes, N. 1990. Measurement of plasma progesterone concentrations by extraction and non-extraction radioimmunoassays. Irish Journal of Agricultural Research 29: 109–115.Google Scholar
Savio, J. D., Keenan, L., Boland, M. P. and Roche, J. F. 1988. Pattern of growth of dominant follicles during the oestrous cycle of heifers. Journal of Reproduction and Fertility 83: 663–671.CrossRefGoogle ScholarPubMed
Savio, J. D., Boland, M. P., Hynes, N. and Roche, J. F. 1990. Resumption of follicular activity in the early post-partum period of dairy cows. Journal of Reproduction and Fertility 88: 569–579.CrossRefGoogle ScholarPubMed
Sirois, J. and Fortune, J. E. 1988. Ovarian follicular dynamics during the estrous cycle in heifers monitored by real-time ultrasonography. Biology of Reproduction 39: 308–317.CrossRefGoogle ScholarPubMed
Snedecor, G. W. and Cochran, W. G. 1989. Statistical methods, eighth edition. Iowa State University Press, Ames, IA.Google Scholar
Sunderland, S. J., Crowe, M. A., Boland, M. P., Roche, J. F. and Ireland, J. J. 1994. Selection, dominance and atresia of follicles during the oestrous cycle of heifers. Journal of Reproduction and Fertility 101: 547–555.CrossRefGoogle ScholarPubMed
Wettemann, R. P. and Castree, J. W. 1994. Immunization of heifers against gonadotropin releasing hormone delays puberty and causes the cessation of estrous cycles. Animal Reproduction Science 36: 49–59.CrossRefGoogle Scholar