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Expression of GHR and PGC-lα in association with changes of MyHC isof orm types in longissimus muscle of Erhualian and Large White pigs (Sus scrofa) during postnatal growth

  • Ru-Qian Zhao (a1), Xiao-Jing Yang (a1), Qin-Fu Xu (a1), Xi-Hui Wei (a1), Dong Xia (a1) and Jie Chen (a1)...

Abstract

Myofibre composition in longissimus dorsi (LD) muscle of Erhualian (EHL) and Large White (LW) pigs was investigated by determining the ratios of mRNA abundances of four myosin heavy chain (MyHC) isoforms (MyHC I, MyHC 2a, MyHC 2x and MyHC 2b) using multiplex RT-PCR. The relationship between expression of growth hormone receptor (GHR) and peroxisome proliferator-activated receptor α coactivator-lα (PGC-lα) mRNAs and changes ofmyofibre type composition during postnatal growth from 3 to 180 days of age were analysed. At 3 days of age, proportions of MyHC I, 2a and 2x fibres were high while only a few MyHC 2b fibres were differentiated. Dramatic changes were observed from day 3 to day 20 with significantly decreased MyHC I, 2a and 2x fibres but abrupt increases in MyHC 2b fibres in both breeds of pigs. Breed difference was exhibited only from day 90, with higher MyHC 2b but less MyHC I and 2a expression in LW pigs. Developmental pattern of GHR mRNA expression in LD muscle coincided with that of MyHC 2b, with LW pigs expressing higher abundance of GHR mRNA. PGC-lα mRNA expression followed distinct patterns in the two breeds of pigs, a higher level of PGC-lα mRNA being expressed in LD muscle of EHL pigs, the breed that possessed more oxidative fibres (MyHC I and MyHC 2a).

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Aroniadou-Anderjaska, V., Lemon, P. W. and Gilloteaux, J. 1996. Effects of exogenous growth hormone on skeletal muscle of young female rats. Tissue Cell 28: 719724.
Ashmore, C. R., Tompkins, G. and Doerr, L. 1972. Postnatal development of muscle fiber types in domestic animals. Journal of Animal Science 34:3741.
Baar, K., Wende, A. R., Jones, T. E., Marison, M., Nolte, L. A., Chen, M., Kelly, D. P. and Holloszy, J. O. 2002. Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1. FASEB Journal 16: 18791886.
Brameld, J. M., Atkinson, J. L., Saunders, J. C., Pell, J. M., Buttery, P. J. and Gilmour, R. S. 1996. Effects of growth hormone administration and dietary protein intake on insulin-like growth factor I and growth hormone receptor mRNA expression in porcine liver, skeletal muscle, and adipose tissue. Journal of Animal Science 74:18321841.
Casse, A. H., Desplanches, D., Mayet-Sornay, M. H., Raccurt, M., Jegou, S. and Morel, G. 2003. Growth hormone receptor expression in atrophying muscle fibers of rats. Endocrinology 144:36923697.
Chang, K. C., Costa, N. D., Blackley, R. and Evans, G. P. G. W. J. D. 2003. Relationships of myosin heavy chain fibers types to meat quality traits in traditional and modern pigs. Meat Science 64:93103.
Chikuni, K., Tanabe, R., Muroya, S. and Nakajima, I. 2001. Difference in molecular structure among the porcine myosin heavy chain 2a, 2x, and 2b isoforms. Meat Science 57: 311317.
Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Analytical Biochemistry 162:156159.
Cioffi, J. A., Wang, X. and Kopchick, J. J. 1990. Porcine growth hormone receptor cDNA sequence. Nucleic Acids Research 18:6451.
Combes, S., Louveau, I. and Bonneau, M. 1997. Effect of GH administration on GH and IGF-I receptors in porcine skeletal muscle and liver in relation to plasma GH-binding protein. Journal of Endocrinology 155:1926.
Cooper, C. C., Cassens, R. G., Kastenschmidt, L. L. and Briskey, E. J. 1970. Histochemical characterization of muscle differentiation. Developmental Biology 23:169184.
Czerwinski, S. M. and Martin, J. M. 1994. Effect of somatotropin on myosin expression in pigs. Journal of Animal Science 72:12041208.
da Costa, N., Blackley, R., Alzuherri, H. and Chang, K. C. 2002. Quantifying the temporospatial expression of postnatal porcine skeletal myosin heavy chain genes. Journal of Histochemistry, and Cytochemistry 50:353364.
Eggert, J. M., Depreux, F. F. S., Schinckel, A. P., Grant, A. L. and Gerrard, D. E. 2002. Myosin heavy chain isoforms account for variation in pork quality. Meat Science 61:117126.
Etherton, T. D. and Bauman, D. E. 1998. Biology of somatotropin in growth and lactation of domestic animals. Physiology Reviews 78: 745761.
Fiedler, I., Nuernberg, K., Hardge, T., Nuernberg, G. and Ender, K. 2003. Phenotypic variations of muscle fibre and intramuscular fat traits in longissimus muscle of F2 population Duroc X Berlin miniature pig and relationshps to meat quality. Meat Science 63:131139.
Gil, M., Oliver, M. A., Gispert, M., Diestre, A., Sosnicki, A. A., Lacoste, A. and Carrion, D. 2003. The relationships between pig genetics, myosin heavy chain I biochemical traits and quality of M. longissimus thoracis. Meat Science 65: 10631070.
Goto, M., Terada, S., Kato, M., Katoh, M., Yokozeki, T., Tabata, I. and Shimokawa., T. 2000. cDNA cloning and mRNA analysis of PGC-1 in epitrochlearis muscle in swimming-exercised rats. Biochemical and Biophysical Research Communications 274:350354.
Hammelainen, N. and Pette, D. 1993. The histochemical profiles of fast fiber types IIB, IID, and IIA in skeletal muscles of mouse, rat, and rabbit. Journal of Histochemistry and Cytochemistry 41: 733743.
Harrison, A. P., Latorre, R. and Dauncey, M. J. 1997. Postnatal development and differentiation of myofibres in functionally diverse porcine skeletal muscles. Reproduction, Fertility and Development 9: 731740.
Karlsson, A. H., Klont, R. E. and Fernandez, X. 1999. Skeletal muscle fibres as factors for pork quality. Livestock Production Science 60:255269.
Klont, R. E., Brocks, L. and Eikelenboom, G. 1998. Muscle fiber type and meat quality. Meat Science 49: S219-S229.
Knutti, D. and Kralli, A. 2001. PGC-1, a versatile coactivator. Trends in Endocrinology and Metabolism 12: 360365.
Lefaucheur, L., Ecolan, P., Lossec, G., Gabillard, J. C., Butler-Browne, G. S. and Herpin, P. 2001. Influence of early postnatal cold exposure on myofiber maturation in pig skeletal muscle. Journal of Muscle Research and Cell Motility 22: 439452.
Lefaucheur, L., Ecolan, P., Plantard, L. and Gueguen, N. 2002. New insights into muscle fiber types in the pig. Journal of Histochemistry and Cytochemistry 50: 719730.
Lewis, A. J., Wester, T. J., Burrin, D. G. and Dauncey, M. J. 2000. Exogenous growth hormone induces somatotrophic gene expression in neonatal liver and skeletal muscle. American Journal of Physiology - Regulatory Integrative and Comparative Physiology 278: R838-R844.
Lin, J., Wu, H., Tarr, P. T., Zhang, C. Y., Wu, Z., Boss, O., Michael, L. F., Puigserver, P., Isotani, E., Olson, E. N., Lowell, B. B., Bassel-Duby, R. and Spiegelman, B. M. 2002. Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres. Nature 418: 797801.
Lu, B. D., Allen, D. L., Leinwand, L. A. and Lyons, G. E. 1999. Spatial and temporal changes in myosin heavy chain gene expression in skeletal muscle development. Developmental Biology 216:312326.
Maltin, C. A., Warkup, C. C., Matthews, R. K., Grant, C. M., Porter, A. D. and Delday, M. I. 1997. Pig muscle fibre characteristics as a source of variation in eating quality. Meat Science 47:237248.
Moody, W. G., Enser, M. B., Wood, J. D., Restall, D. J. and Lister, D. 1978. Comparison of fat and muscle development in Pietrain and Large White piglets. Journal of Animal Science 46:618633.
Muroya, S., Nakajima, I. and Chikun, K. 2002. Related expression of MyoD and Myf5 with myosin heavy chain isoform types in bovine adult skeletal muscles. Zoological Science 19:755761.
Ono, Y., Solomon, M. B., Evock-Clover, C. M., Steele, N. C. and Maruyama, K. 1995. Effects of porcine somatotropin administration on porcine muscles located within different regions of the body. Journal of Animal Science 73:22822288.
Picard, B., Lefaucheur, L., Bern, C. and Duclos, M. J. 2002. Muscle fibre ontogenesis in farm animal species. Reproduction, Nutrition, Development 42:415431.
Puigserver, P., Wu, Z., Park, C. W., Graves, R., Wright, M. and Spiegelman, B. M. 1998. A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell 92:829839.
Rehfeldt, C. and Ender, K. 1993. Skeletal muscle cellularity and histochemistry in response to porcine somatotropin in finishing pigs. Meat Science 34:118.
Russell, A. P., Feilchenfeldt, J., Schreiber, S., Praz, M., Crettenand, A., Gobelet, C., Meier, C. A., Bell, D. R., Kralli, A., Giacobino, J. P. and Deriaz, O. 2003. Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-gamma coactivator-1 and peroxisome proliferator-activated receptor-alpha in skeletal muscle. Diabetes 52: 28742881.
Schiaffino, S. and Reggiani, C. 1994. Myosin isoforms in mammalian skeletal muscle. Journal of Applied Physiology 77: 493501.
Solomon, M. B., Caperna, T. J., Mroz, R. J. and Steele, N. C. 1994. Influence of dietary protein and recombinant porcine somatotropin administration in young pigs. III. Muscle fiber morphology and shear force. Journal of Animal Science 72: 615621.
Sorensen, M. T., Oksbjerg, N., Agergaard, N. and Petersen, J. S. 1996. Tissue deposition rates in relation to muscle fibre and fat cell characteristics in lean female pigs (Sus scrofa) following treatment with porcine growth hormone (pGH). Comparative Biochemistry and Physiology. A. Physiology 113:9196.
Statistical Packages for the Social Sciences. 2000. User's guide 11. O for Windows. SPSS Inc., Chicago.
Tanabe, R., Muroya, S. and Chikuni, K. 1999. Expression of myosin heavy chain isoforms in porcine muscles determined by multiplex PCR. Journal of Food Science 64: 222225.
Terada, S., Goto, M., Kato, M., Kawanaka, K., Shimokawa, T. and Tabata, I. 2002. Effects of low-intensity prolonged exercise on PGC-1 mRNA expression in rat epitrochlearis muscle. Biochemical and Biophysical Research Communications 296: 350354.
Wegner, J., Albrecht, E., Fiedler, I., Teuscher, F., Papstein, H. J. and Ender, K. 2000. Growth- and breed-related changes of muscle fiber characteristics in cattle. Journal of Animal Science 78:14851496.
Weiss, A. and Leinwand, L. A. 1996. The mammalian myosin heavy chain gene family. Annual Review of Cell and Developmental Biology 12:417439.
Wigmore, P. M. and Stickland, N. C. 1983. Muscle development in large and small pig fetuses. Journal of Anatomy 137: 235245.
Zhao, R. Q., Zhou, J., Xu, Q. E., Zhao, Z. H., Wei, X. H., Xia, D. and Chen, W. H. 2003. Tissue-specific effect of recombinant porcine growth hormone on expression of growth-related genes in the pig. Journal of Animal and Feed Science 12: 595605.

Keywords

Expression of GHR and PGC-lα in association with changes of MyHC isof orm types in longissimus muscle of Erhualian and Large White pigs (Sus scrofa) during postnatal growth

  • Ru-Qian Zhao (a1), Xiao-Jing Yang (a1), Qin-Fu Xu (a1), Xi-Hui Wei (a1), Dong Xia (a1) and Jie Chen (a1)...

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