Skip to main content Accessibility help

The effects of varying dose and pattern of administration of ovine FSH on the response to superovulation in performance tested, juvenile Simmental heifers

  • P.J. Broadbent (a1), L.D. Tregaskes (a1), D.F. Dolman (a1) and A.K. Smith (a1)


The response of performance tested purebred Simmental heifers to various superovulatory treatments with ovine FSH was examined in two experiments. The heifers were 12 months old at embryo recovery, had average live weights of 468·4 kg (experiment 1) and 493·2 kg (experiment 2), and were fat (body condition score approx.4·0 to 4·5 units) at embryo recovery. In experiment 1, the effect of administering a total of 9·0 or 10·8 mg ovine FSH (Ovagen) administered as eight equal doses twice daily over 4 days was evaluated. In experiment 2, a total of 9·0 mg ovine FSH was administered either in equal doses in a level pattern or in declining doses twice daily over 4 days.

The response to the low, compared with the high, dose of ovine FSH in experiment 1 was 8·8 v. 10·5 ovulations (corpora lutea); 7·7 v. 7·7 totalova plus embryos recovered; 6·0 v. 4·1 viable embryos; 4·4 v. 3·1 grade 1 embryos (P > 0·05); and 1·7 v. 4·1 non-fertile ova(F < 0·01). In experiment 2, the response to the same total dose of ovine FSH administered in a level compared to a declining pattern was 8·5 v. 10·7 ovulations; 5·6 v. 9·6 total ova plus embryos; 3·1 v. 5·6 viable embryos; 2·5 v. 3·8 grade 1 embryos; and 1·7 v. 2·2 non-fertile ova. It was concluded that, although the differences between the treatments in the yields of viable and grade 1 embryos were not significantly different, there are practical and economic advantages to using a low rather than a high level of gonadotropin and there are no marked disadvantages in these areas to administering ovine FSH in a declining rather than a level pattern of doses.



Hide All
Alfuraiji, M. M. 1989. The use of monoclonal antibody to pregnant mare serum gonodotrophin in superovulation of cattle. PhD thesis, Universityof Aberdeen.
Alfuraiji, M. M., Atkinson, T., Broadbent, P. J. and Hutchinson, J.S.M. 1993. Superovulation in cattle using PMSG followed by PMSG monoclonal antibodies. Animal Reproduction Science 33: 99109.
Armstrong, D. T. and Leung, P. C. K. 1990. The physiological basis of superovulation. Seminars in Reproductive Endocrinology 8: 219231.
Bielanski, A. and Yadav, B. R. 1990. A note on fertilization and embryo production in superovulated cattle with various levels of subcutaneous fat tissue. Animal Production 51:426430.
Callesen, H., Greve, T. and Hyttel, P. 1987. Premature ovulations in superovulated cattle. Theriogenology 28:155166.
Chupin, D., Cognie, Y., Combarnous, Y., Procureur, R. and Saumande, J. 1987. Effect of purified LH and FSH on ovulation in the cow and ewe. In Follicular growth and ovulation rate in farm animals (ed. Roche, J. F. and O'Callaghan, D.), pp. 6672. Martinus Nijhoff, The Hague.
El-Banna, A.A. and Hafez, E. S. E. 1970. Sperm transport and distribution in rabbit and cattle female tract. Fertility and Sterility 21: 534540.
Elsden, R. P., Nelson, L. D. and Seidel, G. E. 1978. Superovulation of cows with follicle stimulating hormone and pregnant mare's serum gonadotrophin. Theriogenology 9:1726.
Folley, S. J. and Malpress, F. H. 1944. The response of the bovine ovary to pregnant mares' serum and horse pituitary extract. Proceedings of the Royal Society 132:164188.
Gonzalez, A., Lussier, J. G., Carruthers, T. D., Murphy, B. D. and Mapletoft, R. J. 1990. Superovulation of beef heifers with Folltropin: a new FSH preparation containing reduced LH activity. Theriogenology 33: 519529.
Hasler, J. F., Brooke, G. P. and McCauley, A. D. 1981. The relationship between age and response to superovulation in Holstein cows and heifers. Theriogenology 15: 109 (abstr.).
Hawk, H. W. 1988. Gamete transfer in the superovulated cow. Theriogenology 29: 125142.
Henderson, K. M., Weaver, A., Wards, R. L., Lun, S. and McNatty, K. P. 1990. Comparison of commercial gonadotrophins using bioassays. Proceedings of The New Zealand Society of Animal Production 50: 161165.
Hyttel, P., Callesen, H., Greve, T. and Schmidt, M. 1991. Oocyte maturation and sperm transport in superovulated cattle. Theriogenology 35: 91108.
Hyttel, P., Greve, T. and Callesen, H. 1988. Ultrastructure of in vivo fertilization in superovulated cattle. Journal of Reproduction and Fertility 82: 113.
Jainudeen, M. R., Hafez, E. S. E. and Lineweaver, J. A. 1966. Superovulation in the calf. Journal of Reproduction and Fertility 12: 149153.
Kempster, A. J., Cook, G. L. and Southgate, J. R. 1982. A comparison of different breeds and crosses from the suckler herd. 2. Carcass characteristics. Animal Production 35: 99111.
Lerner, S. P., Thayne, W. V., Baker, R. D., Henschen, T., Meredith, S., Inskeep, E. K., Dailey, R. A., Lewis, P. E. and Butcher, R. L. 1986. Age, dose of FSH and other factors affecting superovulation in Holstein cows. Journal of Animal Science 63: 176183.
Lindner, G. M. and Wright, R. W. 1983. Bovine embryo morphology and evaluation. Theriogenology 20: 407416.
Lowman, B. G., Scott, N. A. and Somerville, S. H. 1976. Condition scoring of cattle. Revised edition. Bulletin, East of Scotland College of Agriculture, no. 6.
Marden, W. G. R. 1953. The hormonal control of ovulation in the calf. Journal of Agricultural Science, Cambridge 43: 381412.
Mariana, J. G., Mauleon, P., Benoit, M. and Chupin, D. 1970. Variability and repeatability of the number of ovulations obtained after injection of 1,600 iu PMSG and 1,500 iu hCG. Annales de Biologic Animale, Biochemie, Biophysique 10: 567573.
Moore, N. W. 1975. The control of time of oestrus and ovulation and the induction of superovulation in cattle. Australian journal of Agricultural Research 25: 295304.
Murphy, M. G., Enright, W. J., Crowe, M. A., McConnell, K., Spicer, L. J., Boland, M. P. and Roche, J. F. 1991. Effect of dietary energy intake on pattern of growth of dominant follicles during the oestrous cycle in beef heifers. journal of Reproduction and Fertility 92: 333338.
Murphy, B. D., Mapletoft, R. J., Manns, J. and Humphrey, W. D. 1984. Variability in gonadotrophin preparations as a factor in the superovulatory response. Theriogenology 21: 117125.
Newcomb, R. 1980. Investigation of factors affecting superovulation and non—surgical embryo recovery from lactating British Friesian cows. Veterinary Record 106: 4852.
Newcomb, R., Christie, W. G. and Rowson, L. E. A. 1978. The non—surgical recovery and transfer of bovine embryos. In Control of reproduction in the cow (ed. Sreenan, J. M.), pp.292304. Commission of the European Communities, Luxembourg.
Newcomb, R., Christie, W. B., Rowson, L. E. A., Walters, D. E. and Bousfield, W. E. D. 1979. Influence of dose, repeated treatment and batch of hormone on ovarian response in heifers treated with PMSG. journal of Reproduction and Fertility 56: 113118.
Newcomb, R., Rowson, L. E. A. and Trounson, A. O. 1978. The Sacrewell project: an on farm demonstration of the potential of egg transfer. Veterinary Record 103: 415418.
Porter, S. J., Chadwick, J. P., Owen, M. G. and Page, S. J. 1988. Evaluation of seven ultrasonic machines for estimating carcass composition in live bulls. Animal Production 46: 496 (abstr.).
Sawyer, G. J., Broadbent, P. J. and Dolman, D. F. 1995. Ultrasound—monitored ovarian responses in normal and superovulated cattle given exogenous progesterone at different stages of the oestrous cycle. Animal Reproduction Science 38: 187201.
Snyder, D. A. 1986. Superovulation of cows and heifers selected for twinning. Theriogenology 25: 200 (abstr.).
Tregaskes, L. D., Broadbent, P. J., Roden, J. A., Hutchinson, J. S. M. and Dolman, D. F. 1996. Attainment of puberty and response to superovulation in performance tested Simmental heifers. Animal Science In press.


Related content

Powered by UNSILO

The effects of varying dose and pattern of administration of ovine FSH on the response to superovulation in performance tested, juvenile Simmental heifers

  • P.J. Broadbent (a1), L.D. Tregaskes (a1), D.F. Dolman (a1) and A.K. Smith (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.