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The effect of implantation of trenbolone acetate and oestradiol-17β in wether lambs at two initial live weights on concentrations of steroidal residues and blood glucose, urea and thyroid hormones

Published online by Cambridge University Press:  02 September 2010

Lesley J. MacVinish  and
H. Galbraith
Lesley J. MacVinish
Affiliation:
University of Aberdeen, School of Agriculture, 581 King Street, Aberdeen AB9 1UD
H. Galbraith
Affiliation:
University of Aberdeen, School of Agriculture, 581 King Street, Aberdeen AB9 1UD
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Abstract

Thirty-two Border Leicester ♂ × Scottish Blackface ♀ wether lambs aged about 5 months were divided into two groups on the basis of live weight such that group Gl contained the 16 lightest lambs and group G2 the 16 heaviest. Lambs in group Gl were subdivided equally at random either to be sham-implanted controls (group C1) or to be implanted with 35 mg trenbolone acetate (TBA) + 5 mg oestradiol-17β (group T1) at 24 kg initial live weight. The lambs in group G2 were also subdivided into two groups (groups C2 and T2) and similarly treated approximately 1 month later at 37 kg initial live weight. The lambs were offered ad libitum a good-quality diet. They were slaughtered 60 days after implantation. Comparisons were made for the main effects of hormonal treatment and initial live weight.

Concentrations in blood of 17-β-hydroxy-trenbolone (TBOH) and oestradiol-17β (OE) measured by radioimmunoassay peaked within 1 to 3 weeks after implantation and declined thereafter. Maximum concentrations and concentrations at slaughter respectively were 1·46 and 0·32 μg/l (group T1) and 0·78 and 0·28 μg/l (T2) for TBOH and 85 and 33 μg/1 (T1) and 59 and 37 ng/l (T2) for OE. Values up to week 7 were consistently greater in implanted animals in group T1 than in group T2. Hormonal implantation decreased the concentrations of total plasma triiodothyronine and thyroxine and urea and increased values for glucose up to week 5 or 6 after implantation. The animals in group G1 compared with G2 had, on average, variably lower concentrations in plasma of triiodothyronine, thyroxine, glucose and urea.

The highest concentrations of solvent-extractable residues were obtained in samples of kidney and liver (up to about 500 ng/kg for TBOH and 180 ng/kg for OE) with intermediate levels for fat and lowest levels for muscle. Conjugated trenbolone ranging from 45 to 186 ng/kg was present in samples of kidney, liver and perinephric fat. Trenbolone acetate was detected only in samples of fat. Variable effects of live weight at implantation on residue levels were recorded.

Type
Research Article
Information
Animal Production , Volume 47 , Issue 1 , August 1988 , pp. 75 - 85
Copyright
Copyright © British Society of Animal Science 1988

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References

Dixon, S. N. and Heitzman, R. J. 1983. Measurements of synthetic anabolic agents in the tissues of farm animals. In Anabolics in Animal Production (ed. Meissonier, E.), pp. 381–392. Office International des Epizooties, Paris.Google Scholar
Donaldson, I. A., Hart, I. C. and Heitzman, R. J. 1981. Growth hormone, insulin, prolactin and total thyroxine in the plasma of sheep implanted with the anabolic steroid trenbolone acetate alone or with oestradiol. Research in Veterinary Science 30: 7–13.CrossRefGoogle ScholarPubMed
Galbraith, H. 1980. The effect of trenbolone acetate on growth, blood hormones and metabolites and nitrogen balance of beef heifers. Animal Production 30: 389–394.Google Scholar
Heitzman, R. J. 1986. Safety aspects of growth manipulation in animals. In Control and Manipulation of Animal Growth (ed. Buttery, P. J., Haynes, N. B. and Lindsay, D. B.), pp. 315–330. Butterworths, London.CrossRefGoogle Scholar
Heitzman, R. J., Donaldson, I. A. and Hart, E. C. 1980. Effect of anabolic steroids on plasma thyroid hormones in steers and heifers. British Veterinary Journal 136: 168–174.CrossRefGoogle ScholarPubMed
Heitzman, R. J. and Harwood, D. J. 1977. Residue levels of trenbolone and oestradiol-17β in plasma and tissues of steers implanted with anabolic steroid preparations. British Veterinary Journal 133: 564–571.CrossRefGoogle ScholarPubMed
Henricks, D. M., Gray, S. L. and Hoover, L. B. 1983. Residue levels of endogenous oestrogens in beef tissues. In Anabolics in Animal Production (ed. Meissonier, E.), pp. 233–248. Office International des Epizooties, Paris.Google Scholar
Henricks, D. M. and Torrence, A. K. 1978. Endogenous estradiol-17β in bovine tissues. Journal of the Association of Official Analytical Chemists 61: 1280–1283.Google ScholarPubMed
Hoffman, B. 1978. Use of radioimmunoassay for monitoring hormonal residues in edible animal products. Journal of the Association of Official Analytical Chemists 61: 1263–1273.Google Scholar
Hoffman, B. and Oettel, G. 1976. Radioimmunoassays for free and conjugated trienbolone and for trienbolone acetate in bovine tissue and plasma samples. Steroids 24: 504–523.Google Scholar
Jououey, A., Mouren, M. and Salmon, J. 1983. Analytical methods for trenbolone. In Anabolics in Animal Production (ed. Meissonier, E.), pp. 423–442. Office International des Epizootics, Paris.Google Scholar
MacVinish, L. J. 1983. A study of the concentrations of steroidal compounds and endogenous hormones in sheep implanted with trenbolone acetate alone or combined with oestradiol-17β. Ph.D. Thesis, Aberdeen University.Google Scholar
Meissonnier, E. ed. 1983. Anabolics in Animal Production. Office International des Epizooties, Paris.Google Scholar
Pottier, J., Busigny, M. and Grandadam, J. A. 1975. Plasma kinetics, excretion in milk and tissue levels in the cow following implantation of trenbolone acetate. Journal of Animal Science 41: 962–968.CrossRefGoogle ScholarPubMed
Ratcliffe, W. A., Challand, G. S. and Ratcliffe, J. G. 1974. A critical evaluation of separation methods in radioimmunoassays for total triiodothyronine and thyroxine in unextracted human serum. Annals of Clinical Biochemistry 11: 224–229.CrossRefGoogle ScholarPubMed
Rhs, P. M., Suresh, T. P., Ratten, P. S. and Bouffault, J. C. 1974. Metabolic effects of estradiol and trienbolone. Journal of Animal Science 39: 164 (Abstr.).Google Scholar
Singh, S. B., Scaife, J. R. and Galbraith, H. 1988. Blood and tissue lipid composition and lipase activity in wether lambs treated with trenbolone acetate combined with oestradiol-17β at two different live weights. Animal Production 47: 87–96.Google Scholar
Sulieman, A. H., Galbraith, H. and Topps, J. H. 1986. Growth performance and body composition of early weaned wether lambs treated with trenbolone acetate combined with oestradiol-17β. Animal Production 43: 109–114.Google Scholar
Sulieman, A. H., Galbraith, H. and Topps, J. H. 1988. Growth performance and body composition of wether lambs implanted at two different initial live weights with trenbolone acetate combined with oestradiol-17β. Animal Production 47: 65–74.Google Scholar
Sykes, A. R. 1976. An assessment of the value of plasma urea nitrogen and albumin concentrations as monitors of the protein status of sheep. In The Use of Blood Metabolites in Animal Production (ed. Lister, D.), Occasional Publication, British Society of Animal Production, No. 1, pp. 143–154.Google Scholar